Comparison of the protein patterns of Erysipelothrix rhusiopathiae strains by SDS-PAGE and autoradiography.

The proteins of 12 Erysipelothrix rhusiopathiae strains were radiolabelled with L-[35S] methionine, and the protein fractions were detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and autoradiography. The strains were found to differ from each other in their protein patterns; thus, it was concluded that the method applied could provide useful data for the identification of strains. The described procedure was used for determining the percentage contents of protein fractions of identical molecular mass within the strains compared. The results show that there is no significant correlation between the type of the strains and the percentage of identical molecular weight fractions.

Comparative studies on different methods in typing strains of erysipelothrix rhusiopathiae. I. Methods and influence of some factors on their results.

To contribute to the standardization of typing Erysipelothrix rhusiopathiae strains the following points have been examined and compared: the usefulness of tube precipitation, paper chromatography and agar gel diffusion; different methods to produce good antisera (specific high titres) and antigens (CH3COOH-, HCl-extract, extraction by means of autoclavation). Rabbits have a considerably varying individual ability to produce Erysipelas antibodies with good precipitation titres. The antigenic effect of different E.rh.-strains and of dissociated forms is not equal. The density of bacteria suspension for extract production and for rabbit immunization has to be sufficient. The period of usability of extracts and antisera is different.

Comparative studies on different methods in typing strains of erysipelothrix rhusiopathiae. II. Comments on the methods and type classification tests of E. rhusiopathiae strains.

The tube precipitation method with antigen produced from autoclaved organisms and those produced by CH3COOH extraction and with good antisera have proved to give the best results and should be preferred at least in the routine work. It is a quick method but the results cannot be fixed and later used in documentation. - The paper chromatographic method is very sensitive, makes much work and heavy demands on the clarity of the antigen and serum and reading the test. The results can be fixed and used in documentation. The agar gel diffusion method demands more work than the other methods but the preparation can be stained and stored, the precipitation lines can reveal differences in antigenic structure of different strains. Erysipelas strains world-wide known as standard strains of the individual types have been examined. Some changes in the type classification are recommended. Signs of antigen differences of strains of the same type and mutual antigens in strains of different types can be detected in the results.

[Studies of the occurrence of erysipelas bacteria in healthy swine in the Hungarian People's Republic with reference to possible use of erysipelas live vaccines]. / Untersuchungen über das Vorkommen von Rotlaufbakterien bei gesunden Schweinen in der Ungarischen Volksrepublik im Hinblick auf den Einsatz von Rotlauf-Lebendimpfstoffen.

The number of pigs for slaughter which carried erysipelas bacteria has gone down continuously in Hungary. The incidence of erysipelas declined in parallel. That improving trend has been attributed to better conditions of large-stock keeping and to the exclusive use of inactivated vaccine. Careful consideration should be given to epizootiological aspects as well as to the environment, whenever live vaccines are to be used. Veterinarians bear growing responsibility in that particular context.