ABSTRACT
A system for time-resolved fluoroimmunoassay (TR-FIA) is developed for detecting early IgG during the active stage of cytomegalovirus infection on the basis of CMV-control enzyme immunoassay system. The antigen is cloned and expressed in E. coli recombinant main immediate early protein of human CMV. The active form of CMV infection was detected additionally in 5 out of 25 women who gave birth to sick babies by means of the new test system in complex with other laboratory diagnostic methods. The test is recommended for prenatal diagnosis of active CMV infection in pregnant women and for predicting the risk of neonatal disease.
Subject(s)
Cytomegalovirus Infections/diagnosis , Fluorescent Antibody Technique , Immediate-Early Proteins/immunology , Infant, Newborn, Diseases/diagnosis , Cytomegalovirus Infections/congenital , Cytomegalovirus Infections/immunology , Female , Humans , Infant, Newborn , Infant, Newborn, Diseases/immunology , Metals, Rare Earth , Pregnancy , Prenatal DiagnosisABSTRACT
On the basis of the lanthanide immunofluorescent assay (LIFA) test systems for the determination of herpes simplex virus (HSV-LIFA) and cytomegalovirus (CMV-LIFA) antigens have been developed. The test system HSV-LIFA includes the sandwich of monoclonal antibodies (McAb) HSV A.3.3. or B-11 to type 2 HSV, strain BH; the test system CMV-LIFA includes the sandwich of rabbit McAb to CMV, strain AD 169. The approbation of the test systems has revealed that they insure the specific detection of HSV and CMV antigens in clinical specimens (urine, blood, liquor, saliva), the LIFA results well correlating with the data on the isolation of viruses in cell cultures, with the results obtained by other diagnostic methods and with clinical manifestations of diseases. LIFA has been shown to be more sensitive than the enzyme immunoassay.
Subject(s)
Antigens, Viral/analysis , Cytomegalovirus/immunology , Fluorescent Antibody Technique , Herpesvirus 1, Human/immunology , Metals, Rare Earth , Reagent Kits, Diagnostic , Animals , Body Fluids/immunology , Evaluation Studies as Topic , Humans , Hybridomas/immunology , Mice , Rabbits , Reagent Kits, Diagnostic/statistics & numerical data , Sensitivity and SpecificitySubject(s)
Bacterial Infections , Brain Diseases , Fetal Diseases , Virus Diseases , Bacterial Infections/diagnosis , Bacterial Infections/etiology , Bacterial Infections/mortality , Bacterial Infections/therapy , Brain Diseases/diagnosis , Brain Diseases/etiology , Brain Diseases/mortality , Brain Diseases/therapy , Critical Illness/mortality , Critical Illness/therapy , Fetal Diseases/diagnosis , Fetal Diseases/etiology , Fetal Diseases/mortality , Fetal Diseases/therapy , Humans , Infant, Newborn , Virus Diseases/diagnosis , Virus Diseases/etiology , Virus Diseases/mortality , Virus Diseases/therapyABSTRACT
The incidence of cysts of cerebral ventricular vascular plexuses (CVP), estimated with the use of echography in 2671 newborns, has made up 4.16% in full-term babies and 3.67% in preterm ones. Analysis of 40 CVP cases has shown that asphyxia and marked mechanical effects on fetal head and neck at birth and intrauterine herpetic infection may be conducive to the development of this condition. Psychomotor development of these children in the first 3 years of life was normal and only if CVP were combined with other diseases inducing stable brain injuries, neurologic disorders were present.
Subject(s)
Brain Diseases/diagnostic imaging , Cerebral Ventricles/blood supply , Cysts/diagnostic imaging , Brain Diseases/congenital , Brain Diseases/epidemiology , Cerebral Ventricles/diagnostic imaging , Cysts/congenital , Cysts/epidemiology , Echoencephalography , Humans , Incidence , Infant, Newborn , Infant, Premature , Infant, Premature, Diseases/diagnostic imaging , Infant, Premature, Diseases/epidemiology , Psychomotor Performance , Risk FactorsABSTRACT
Generalized herpetic infection in the newborn runs its course in association with cerebral lesions as well as with injuries to the lungs, heart and liver. The infection diagnosis should take into account the mother's epidemiological anamnesis, the clinical manifestations in the child and the results of the simultaneous use of several methods (fluorescent antibody test, IEA, virological test). Of paramount importance is the simultaneous examination of the mother. Of no less importance is echoencephalography, namely the detection of cysts in white matter of the frontal lobes.
