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1.
Klin Lab Diagn ; 60(4): 62-4, 2015 Apr.
Article in Russian | MEDLINE | ID: mdl-26189294

ABSTRACT

The issue of identification and differentiation of large group of bacteriophages of human pathogenic vibrio is still unresolved. In research and practical applied purposes it is important to consider characteristics of bacteriophages for establishing similarity and differences between them. The actual study was carried out to analyze specimens of DNA-containing bacteriophages of pathogenic vibrio. The overwhelming majority of them characterized by complicated type of symmetry--phages with double-helical DNA and also phages with mono-helical DNA structure discovered recently in vibrio. For the first time, the general framework of identification and differentiation of bacteriophages of pathogenic vibrio was developed. This achievement increases possibility to establish species assignment of phages and to compare with phages registered in the database. "The collection of bacteriophages and test-strains of human pathogenic vibrio" (No2010620549 of 24.09.210).


Subject(s)
Bacteriophage Typing/methods , Bacteriophages , DNA Viruses , Vibrio/classification , Vibrio/virology , Humans
2.
Article in Russian | MEDLINE | ID: mdl-25286530

ABSTRACT

AIM: Comparative evaluation of biological properties of parahemolytic vibrios that had determined outbreaks and sporadic cases of food toxic infection in Primorsky Region in 2012 and previous years. Materials AND METHODS: 40 clinical strains of Vibrio parahaemolyticus isolated in 2012 were studied in comparison with 62 strains from this region that had been characterized by us previously. Virulence was evaluated by a complex method: hemolytic activitywas determined in Kanagawa test (KT), urease - in Kristensen medium. Serotyping was carried out by a commercial kit of O/K sera. PCR-genotyping was carried out by marker genes of 7 pathogenicity "islands" (VPaI-1-7). RESULTS: All the strains isolated from patients in 2012 had KT-positive and urease-negative phenotype, belonged to O3:K6 serogroup and contained marker genes of 7 VPal that allowed to consider them members of a "pandemic" clone as the other clinical strains from this region. However among 2012 strains an increase of number of antibiotic-resistant variants was established compared with 1997 isolates. CONCLUSION: The data obtained give evidence on the risk of spread of a "pandemic" clone of V. parahaemolyticus in the Far-Eastern region of Russia, a dangerous tendency of antibiotic-resistant variant formation and a necessity to monitor morbidity and the environment with mandatory PCR-detection of genes associated with virulence including integrated into pathogenicity "islands".


Subject(s)
Disease Outbreaks , Vibrio Infections/epidemiology , Vibrio parahaemolyticus/pathogenicity , Humans , Polymerase Chain Reaction , Russia , Serotyping , Vibrio Infections/microbiology , Vibrio parahaemolyticus/isolation & purification
3.
Klin Lab Diagn ; (8): 52-3, 2013 Aug.
Article in Russian | MEDLINE | ID: mdl-24340749

ABSTRACT

The sample included five indicator pseudotuberculosis strains. The application of these strains permitted to isolate out of 161 strains of Y. pseudotuberculosis 9 bacteriophages identical by their morphologic and serologic characteristics but having individual particularities in their lytic activity. The test on sensitivity to bacteriophages can be used in laboratory diagnostic to differentiate the strains of Yersinia pseudotuberculosis.


Subject(s)
Bacteriophages/isolation & purification , Bacteriophages/physiology , Yersinia pseudotuberculosis Infections/diagnosis , Yersinia pseudotuberculosis/isolation & purification , Yersinia pseudotuberculosis/virology , Humans , Yersinia pseudotuberculosis Infections/virology
4.
Klin Lab Diagn ; (3): 50-2, 2011 Mar.
Article in Russian | MEDLINE | ID: mdl-21574461

ABSTRACT

Lysogeny was studied in Vibrio mimicus; the indicator V. cholerae El Tor strain was selected to identify phages. New V. mimicus phages were obtained and identified, which had a morphological similarity and an antigen affinity for morphological group I cholerae phages. Phage differentiation revealed that morphological group I V. mimicus phages showed certain differences manifested as their lytic activity against V. cholerae strain 1322-69 of serovar 37 while this property was absent in cholerae phages.


Subject(s)
Bacteriophages/genetics , Bacteriophages/isolation & purification , Lysogeny , Vibrio mimicus/genetics , Vibrio mimicus/virology , Cholera/microbiology , Humans , Vibrio cholerae/virology
5.
Klin Lab Diagn ; (4): 43-5, 2010 Apr.
Article in Russian | MEDLINE | ID: mdl-20524344

ABSTRACT

Five indicator strains of Yersinia enterocolitica 01, 03, 05, 012 serovars were selected, which were used to isolate 7 bacteriophages from 227 Y. enterocolitica strains and 2 bacteriophages from wastewater samples. The specificity of the antigenic composition (3 serovars) and the morphology of phage particles (3 morphological groups) were employed to identify bacteriophages; there were differences in the range of lytic activity and resistance to physical and chemical agents. The specific bacteriophage-susceptibility test showed it possible to differentiate Y. enterocolitica strains.


Subject(s)
Bacteriophages/growth & development , Bacteriophages/isolation & purification , Water Microbiology , Yersinia enterocolitica/virology
7.
Article in Russian | MEDLINE | ID: mdl-16028522

ABSTRACT

The results of the serotyping of 244 V. cholerae non O1/O139 cultures isolated from patients in Uzbekistan in 2000 and 2001 are presented. All isolates were studied by the method of molecular probing and in the polymerase chain reaction for the presence of virulence genes and for sensitivity to phages ctx+, ctx- and hemolytic activity. The use of monoreceptor O-sera O2-O83 made it possible to determine vibrios of 32 serogroups with the dominating role in the etiology of acute enteric diseases belonging to serogroups O18, O62, O82, O37. Genes ctx AB were detected in none of the isolates, 5 of them contained gene tcp A. A group of cultures, sensitive to phage ctx+ and belonging mainly to enteropathogenic serogroups, was detected.


Subject(s)
Vibrio Infections/epidemiology , Vibrio cholerae non-O1/classification , Bacteriophages/genetics , Fimbriae Proteins/genetics , Genes, Bacterial , Humans , Molecular Epidemiology , Serotyping , Uzbekistan/epidemiology , Vibrio cholerae non-O1/genetics , Vibrio cholerae non-O1/pathogenicity , Virulence/genetics
8.
Article in Russian | MEDLINE | ID: mdl-12886601

ABSTRACT

The dynamics of the isolation of V. cholerae cultures from various water objects on the territory of Rostov-on-Don during the period of 1994-2001 was analyzed and biological properties of 14 such cultures were studied. In the absence of epidemic complications during the above-mentioned period, a growth in the amount of V. cholerae isolates, serogroups 01 and 0139, including toxigenic V. cholerae 01, was registered. The microbiological and epidemiological aspects of the monitoring of surface reservoirs and sewage were considered and the expediency of the profound and systematic study of its results for epidemiological surveillance on cholera was emphasized.


Subject(s)
Sewage/microbiology , Vibrio cholerae/isolation & purification , Cholera/diagnosis , Cholera/epidemiology , Humans , Population Surveillance , Retrospective Studies , Russia/epidemiology , Serotyping , Vibrio cholerae/classification , Vibrio cholerae/physiology , Water Microbiology
10.
Article in Russian | MEDLINE | ID: mdl-12043149

ABSTRACT

Information on V. cholerae eltor isolated in the focus of cholera in Kazan in 2001 at different periods of the outbreak is presented. The identity of strains isolated from patients, vibriocarriers and environmental objects, including their antibioticograms (sensitivity to cyprofloxacin and resistance to trimethoprim--sulfamethoxazole, streptomycin, furazolidone and nalidixic acid, which may be regarded as markers), is shown. Variable tandem repetitions in the DNA of 30 isolates strains of different origin have been determined. The results of this determination make it possible to classify all these strains as one genotype, which confirms the suggestion on the circulation of one subclone of the infective agent of cholera in the focus. As revealed in this investigation, the isolated strains are labile with respect to diagnostic phage eltor, while ctx+ strains are resistant to phage eltor ctx+.


Subject(s)
Cholera/metabolism , Vibrio cholerae/genetics , Vibrio cholerae/metabolism , Anti-Bacterial Agents/metabolism , Anti-Bacterial Agents/therapeutic use , Biomarkers , Cholera/drug therapy , Cholera/epidemiology , Ciprofloxacin/therapeutic use , Drug Resistance, Microbial/genetics , Furazolidone/metabolism , Genotype , Humans , Nalidixic Acid/metabolism , Russia/epidemiology , Streptomycin/metabolism , Sulfamethoxazole/metabolism , Trimethoprim Resistance/genetics , Vibrio cholerae/classification , Vibrio cholerae/drug effects , Vibrio cholerae/isolation & purification
11.
Article in Russian | MEDLINE | ID: mdl-11550565

ABSTRACT

The comparative evaluation of the diagnostic value of new cholera eltor bacteriophages ctx+ and ctx-, as well as monophages X[symbol: see text]-3, 4, 5, demonstrated their high activity and specificity. Using of these bacteriophages epidemic potential of 95% Vibrio cholerae eltor strains ctx+ and 84.5% of V. cholerae eltor stains ctx- was determined. Commercial monophages X[symbol: see text]-3, 4, 5 were inferior to bacteriophages ctx+ and ctx- in their diagnostic value: only 55% of strains having gene ctxAB were found to be epidemically dangerous, i.e. 45% of strains capable of causing the disease were not detected. On the basis of the results obtained in this investigation cholera eltor bacteriophages ctx+ and ctx- were recommended for introduction into practical use, while further production of cholera diagnostic monophages X[symbol: see text]-3, 4, 5 was recommended to be stopped.


Subject(s)
Vibrio cholerae/classification , Bacteriophage Typing/methods , Sensitivity and Specificity , Vibrio cholerae/pathogenicity
12.
Article in Russian | MEDLINE | ID: mdl-11881491

ABSTRACT

V. parahaemolyticus and V. alginolyticus strains isolated from patients during an outbreak of an acute enteric disease in Vladivostok in 1997 were studied. All strains were found to possess typical taxonomic signs. V. parahaemolyticus isolated from humans had direct heat stable haemolysin exotoxin. The overwhelming majority of these strains belonged to serovar O3K6. Among the cultures under study 7 phage types were determined: phage types 1, 2, 7, 10 in 8 V. parahaemolyticus strains and phage types 2, 4. 5. 7 in 5 V. alginolyticus strains. The diagnostic halophilic phage lyzed vibrios in 30.2% of strains. The cultures under study were found to be highly sensitive to chloramphenicol, cefotaxime, nalidixic acid and cyprofloxacin. The study proved that the outbreak of alimentary toxicoinfection was caused by vibrios of serogroup O3:K6.


Subject(s)
Disease Outbreaks , Foodborne Diseases/epidemiology , Vibrio Infections/epidemiology , Vibrio , Anti-Infective Agents/pharmacology , Bacterial Proteins/analysis , Bacterial Proteins/metabolism , Bacteriolysis , Bacteriophage Typing , Cefotaxime/pharmacology , Cephalosporins/pharmacology , Chloramphenicol/pharmacology , Ciprofloxacin/pharmacology , Enterotoxins/analysis , Enterotoxins/metabolism , Foodborne Diseases/microbiology , Humans , Microbial Sensitivity Tests , Nalidixic Acid/pharmacology , Serotyping , Siberia/epidemiology , Vibrio/classification , Vibrio/drug effects , Vibrio/metabolism , Vibrio/physiology , Vibrio Infections/microbiology
13.
Article in Russian | MEDLINE | ID: mdl-11210629

ABSTRACT

Cholera bacteriophages have been isolated from 27 lysogenic cultures of V. cholerae O139. As shown the pages under study belong to two morphological groups A1 and F1 and serological types II and XII. The use of prophage typing and the sensitivity test to specific phage made it possible to differentiate V. cholerae strains, serogroup O139.


Subject(s)
Bacteriophages/isolation & purification , Vibrio cholerae/virology , Bacteriophages/classification , Humans , Lysogeny , Microscopy, Electron , Vibrio cholerae/isolation & purification
14.
Vopr Virusol ; 44(3): 136-9, 1999.
Article in Russian | MEDLINE | ID: mdl-10392440

ABSTRACT

Conditions of cryostabilization of Yersinia pestis phages preserving their biological properties at very low temperature are studied.


Subject(s)
Bacteriophages/physiology , Yersinia/virology , Bacteriophages/pathogenicity , Cryopreservation , Virulence
15.
Article in Russian | MEDLINE | ID: mdl-9304335

ABSTRACT

The virulent properties of V. cholerae O1 (more than 1000 strains) were studied. For this purpose the following methods were used: the determination of hemolytic activity, the express method based on the determination of the speed of mannite oxidation, complex analysis and polymerase chain reaction (PCR). The comparative analysis of the results of the in vitro determination of virulence revealed that the most frequently occurring strains among the strains isolated from humans were vct+ strains (80.5%), hemolysis-negative strains (83.9%) which were virulent (faintly virulent) as determined by complex analysis (38.2%), cholerogenic as determined by the express method (60.2%). Among the strains isolated from the environment were vct- strains (87.4%), hemolysis-negative strains (63.2%) which were virulent as determined by complex (13.1%), as well as by the express method (74.3%). The data of the determination of the vct gene did not correlate with the results obtained by complex analysis. The use of complex analysis was expedient only in the detection of strains sensitive to eltor phage in the dilution routine test of V. eltor strains. The methods for the determination of virulence, available for practical laboratories, were Grieg's method for the determination of hemolytic activity and express method. PCR proved to reliable in vitro method for the determination of the vct gene.


Subject(s)
Vibrio cholerae/pathogenicity , Bacteriological Techniques , Bacteriophage Typing , Environmental Microbiology , Genes, Bacterial/genetics , Hemolytic Plaque Technique , Humans , Mannitol/metabolism , Polymerase Chain Reaction , Ukraine , Vibrio cholerae/classification , Vibrio cholerae/genetics , Vibrio cholerae/isolation & purification , Virulence/genetics
16.
Article in Russian | MEDLINE | ID: mdl-9245129

ABSTRACT

The results of the phage typing of 164 halophilic vibrios revealed the most frequent combinations of lysing monophages, denoted by numbers: 1 (A, B, C, D), 2 (B, C, D), 3 (B, C) and 4 (C). In accordance with the proposed scheme, the phage typing of 398 cultures from different ecosystems was carried out. Phagovar was determined in 77.1% of the cultures. Most frequently occurred phagovars 1 (31.9%), 4 (15.8%), 3 (6%), and 2 (3.7%). Their proportion was 61%. 11 other phage combination causing the lysis of the cultures constituted 16.1%; 22.9% of the cultures could not be types. The use of the proposed scheme of phage typing permitted the determination of the temporal, regional, ecosystemic features of the circulation of halophilic vibrios in different ecosystems and regions, which was important for epidemiological analysis.


Subject(s)
Bacteriophage Typing/methods , Vibrio/classification , Bacteriolysis , Ecosystem , Environmental Microbiology , Humans , Vibrio/isolation & purification
17.
Mikrobiol Z ; 57(3): 56-64, 1995.
Article in Russian | MEDLINE | ID: mdl-7655658

ABSTRACT

Phage resistance of 225 strains of cholera germs of O1 group obtained from different countries in 1988-1992 has been analyzed. Change of sensitivity to diagnostic phages was mostly connected with the decrease or loss of agglutinability in cholera sera. Phage resistance is rather conditioned by the change of the surface structures of the cell and by further change of phage reception zones. The increase in the number of strains sensitive to diagnostic phages after 6-12 months of storage evidenced for stabilization of cell wall structures and increase of their viability under relatively favourable conditions of storage.


Subject(s)
Bacteriophages/classification , Bacteriophage Typing , Environmental Microbiology , Humans , Lysogeny , Serotyping , Time Factors , Vibrio cholerae/classification , Vibrio cholerae/isolation & purification , Vibrio cholerae/pathogenicity , Vibrio cholerae/virology , Virulence
18.
Article in Russian | MEDLINE | ID: mdl-7771152

ABSTRACT

A high degree of resistance to cholera diagnostic phages and carriership of prophages characteristic of V. cholerae eltor strains vct+ were shown to be the specific features V. cholerae isolated in Daghestan during the period of June-October 1994. Among the strains under study, isolated respectively in 12 and 18 out of 19 regions of Daghestan, a high proportion was found to have resistance to tetracycline (65%) and chloramphenicol (28.6%). Moreover, some strains were found to be resistant to furagin and erythromycin. Out of 242 strains resistant to antibacterial preparations, 163 strains were found to have multiple resistance. Gentamicin, cipropfloxacin and doxicycline were shown to have high in vitro activity with respect to the strains under study.


Subject(s)
Vibrio cholerae/isolation & purification , Anti-Bacterial Agents/pharmacology , Bacteriophage Typing , Carrier State/microbiology , Cholera/microbiology , Dagestan , Drug Resistance, Microbial , Environmental Microbiology , Humans , Microbial Sensitivity Tests , Vibrio cholerae/classification , Vibrio cholerae/drug effects
19.
Zh Mikrobiol Epidemiol Immunobiol ; Suppl 2: 97-101, 1995.
Article in Russian | MEDLINE | ID: mdl-7771163

ABSTRACT

Materials on the import of rarely occurring Vibrio cholerae, not belonging to group O1 of serovar O139, to the territory of Russia are presented. The clinical picture of a cholera case is described and the biological properties of V. cholerae, serovar O139, are presented. A suggestion has been made concerning the appearance of a new V. cholerae serovar, capable of ousting V. cholerae eltor, the cause of the seventh pandemic.


Subject(s)
Cholera/etiology , Vibrio cholerae/classification , Acute Disease , Cholera/diagnosis , Cholera/microbiology , Cholera/therapy , Combined Modality Therapy , Female , Humans , India , Male , Russia , Serotyping , Travel , Vibrio cholerae/isolation & purification
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