[The toxic action of the "diabetogenic factor" metabolite in the body during the development of insulin-dependent diabetes]. / Toksicheskoe deistvie metabolita--"diabetogennogo faktora" v organizme pri razvitii insulinozavisimogo diabeta.

Investigations of "diabetogenic factor" (DgF) (Diploma No. 386, USSR) in the blood of men and animals with insulin-dependent diabetes have been summarized. The nature and properties of the DgF purified preparation have been outlined and its pathophysiological importance characterized. An inhibitor has been found that inactivate DgF when administered to a diseased individual. It was experimentally established that a single transplantation of cultured healthy beta-cells to an animal with diabetes was successful only in the absence of DgF activity. This leads to stable recover of the animal. The data of other authors have also been briefly discussed.

[Prophylactic effect of vitamin A, neutralizing the development of experimental insulin-dependent diabetes in animals]. / Profilakticheskoe deistvie vitamina A, neitralizuiushchego razvitie vorganizme zhivotnykh éksperimental'nogo insulinzavisimogo diabeta.

Vitamin A deficiency contributed to higher incidence of abnormalities in experimental animals with insulin-dependent diabetes induced by alloxan. However, the similar doses of alloxan did not cause diabetes in the animals maintained on a diet containing adequate amounts of vitamin A used for prophylactic purposes for a long time. The natural diabetogenic factor specific to insulin-dependent diabetes was not found in the blood serum of these animals.

[Fibrinolytic and anticoagulant complexes of low-molecular heparin with tuftsin]. / Fibrinoliticheskie i antikoaguliantnye kompleksy nizkomolekuliarnogo geparina s taftsinom.

The complex of immunopeptide taftsin with low-molecular heparin has been obtained. The complex has fibrinolytic and anticoagulant activities in vitro and in vivo after the injection to albino rats.

[Low-molecular heparin complex formation with the blood coagulation proteins--thrombin and fibrinogen]. / Kompleksoobrazovanie nizkomolekuliarnogo geparina s belkami svertyvaniia krovi--trombinom i fibrinogenom.

It has been found that low molecular heparin (LMH) forms complexes with fibrinogen and thrombin. The formation of the heparin-fibrinogen and heparin-thrombin complexes has been testified by cross-linked electrophoresis. The reaction of complex formation was carried out at variable weight ratios of the components, i.e. 1:3 and 1:6, respectively. This complex causes lysis of unstabilized clots of fibrin. All these complexes manifested a slight anticoagulative activity.

[Fibrinolytic complexes of low-molecular heparin and acetylsalicylic acid]. / Fibrinoliticheskie kompleksy nizkomolekuliarnogo geparina s atsetilsalitsilovoi kislotoi.

Complexes of low-molecular heparin with acetylsalicilic acid was formed in vitro when the weight ratio of components was 1:1, 1:5 and 5:1, respectively. All the complexes possessed fibrinolytic and anticoagulating activities. The complex possessed the highest activity when the ratio of heparin to acetylsalicilic acid was 5:1. This complex at a dose 1 mg/200 g of rat weight had the largest thrombolytic effect on the experimental fresh thrombus.

[A comparative study of the action of preparations of high- and low-molecular weight heparin on hemostatic indices in vitro and in their intravenous administration to animals]. / Sravnitel'noe izuchenie deistviia preparatov vysoko- i nizkomolekuliarnogo geparina na nekotorye pokazateli gemostaza in vitro i pri vnutrivennom vvedenii zhivotnym.

Low-molecular heparin preparations (a Soviet sample and that manufactured by the Celsus Company) in vitro, added to bovine plasma possess a lower antifactor-II activity, less time of recalcification and partial thromboplastin time as compared to high-molecular heparin of equal concentration (mg/ml) or dose (Units/ml). After intravenous injection of low-molecular heparin preparations to animals in a dose of 50 Units/200 g bw the time of hemorrhage was far less, while anticoagulant activity was lower than in animals given the same dose of high-molecular heparin. Both injection of low-molecular and high-molecular heparin results in a rise of the intensity of non-enzymatic fibrinolysis in the animals' blood plasma.

[Heparin from the meadowsweet (Filipendula ulmaria) and its properties]. / Geparin iz tavolgi viazolistnoi, ego svoistva.

In the present work, the nature of an anticoagulant from Philipendula ulmaria was studied. A method for purification of this anticoagulant was developed. Using diverse methods it was shown that the molecular weight, data on element (sulphur, nitrogen, and hydrogen) content, spectral characteristics in the infrared region of the spectrum, and electrophoretic properties of the product indicate its similarity to heparin of animal origin.

[The action of regulator peptides with different biological activities on the process of fibrin-monomer polymerization and on nonenzymatic fibrinolysis]. / Deistvie reguliatornykh peptidov s razlichnoi biologicheskoi aktivnost'iu na protsess polimerizatsii fibrin-monomera i nefermenttativnyi fibrinoliz.

It is shown that preparations of regulatory peptides (RP): interleukin-1, thymoptin, and the endogenous nonpeptide opioid salsalinol produce a marked depolymerization effect on the fibrin-monomer and display nonenzymatic fibrinolytic activity in relation to unstabilized fibrin. Preparations of regulatory peptides thymalin and diphensin of rabbits intensify fibrin polymerization. A single intravenous infusion of diphensin preparation inhibits nonenzymatic fibrinolysis of blood plasma in vivo and in vitro. Repeated intramuscular injections of the immunoactive preparation thymoptin intensify the fibrinolytic properties of blood plasma in rats.

[The blood anticoagulant system in rats perorally administered a heparin-acetylsalicylic acid complex]. / Sostoianie protivosvertyvaiushchei sistemy krovi krys pri peroral'nom vvedenii kompleksa geparin--atsetilsalitsilovaia kislota.

Heparin/acetylsalicylate complexes (1:9 and 10:1) were obtained in vitro. Single or chronic (7-8 days) per os administration to white rats of 0.1% solution of the heparin/acetylsalicylate complex (0.3 ml/200 g body weight) enhanced anticoagulative properties of blood plasma, increased the fibrinolytic activity in respect of stabilized fibrin, and diminished the thrombin-induced platelet aggregation.

[Stable reduction of endogenous insulin production in the body in experimental insulin-dependent diabetes]. / Ustoichivoe vosstanovlenie produktsii éndogennogo insulina v organizme pri éksperimental'nom insulinzavisimom diabete.

Implantation of beta-cells allogenic culture into animals with alloxan diabetes did not produce persistent positive effect. The implanted beta-cells lost their viability as a result of toxic effect of natural diabetogenic factor occurring in blood plasma during insulin-dependent diabetes. Long-term administration of heparin into these animals within first 90 days of the experiment enabled to avoid the negative phenomenon and to neutralize the diabetogenic factor activity. Under these conditions the implanted beta-cells effectively produced endogenous insulin and the symptoms of diabetes disappeared within 14 months.

[The effect of tuftsin on fibrin polymerization in vitro and on the hemostatic system when administered intravenously to healthy animals]. / Vliianie taftsina na polimerizatsiiu fibrina in vitro i sistemu gemostaza pri vnutrivennom vvedenii zdorovym zhivotnym.

Taftsine tetrapeptide has antiprocoagulatory properties in vitro in the presence of plasma. Taftsine exerts depolymerization influence on fibrin monomer in concentrations of 10(-1) to 10(-9) mg/ml. At intravenous injection in doses of 1 mg and 300 ug/kg, taftsine causes the increase in plasma clotting time and significant increase in enzymatic and nonenzymatic fibrinolysis. The effect can be observed during 120 min after injection of the peptide.

[The pancreas as a source of natural diabetogenic factor in alloxan diabetes]. / Podzheludochnaia zheleza kak istochnik estestvennogo diabetogennogo faktora pri alloksanovom diabete.

The results of experimental studies bear evidence that the pancreas of healthy animals produces a humoral factor which differs from insulin and prevents the development of alloxan diabetes. The pancreas of diabetic animals loses the above-mentioned activity and produces into the blood plasma a natural diabetogenic factor which promotes the development of alloxan diabetes.

[The effect of regulatory peptides with various biological activity on the fibrin polymerization process and non-enzymatic fibrinolysis. Hypophyseal and hypophthalamic hormones, liberin, ACTH, and its analogs]. / Deistvie reguliatornykh peptidov s razlichnoi biologicheskoi aktivnost'iu na protsess polimerizatsii fibrina i nefermentativnyi fibrinoliz. Neirogormony gipofiza, gormony gipotalamusa, liberiny, analogi.

Preparations of regulating peptides from hypophysis oxytocin (10(-1)-10(-3) mg/ml) and vasopressin (10(-2)-10(-5) mg/ml) demonstrated weak depolymerizing effect on unstabilized fibrin. Regulating peptides from hypothalamus thyroliberin and PR-546 (10(-1)-10(-3) mg/ml) increased polymerization of fibrin-monomer.

[The antiheparin thrombocyte factor 4 and its interaction with heparin]. / Antigeparinovyi faktor 4 trombotsitov i ego vzaimodeistvie s geparinom.

A rat platelet factor has a high antiheparin activity. It also decreases nonenzymatic fibrinolytic activity of normal rat plasma and antithrombin III-heparin complex. The platelet factor 4 formed inactive complexes with heparin in molar ratios of 1:1 and 2:1. Intravenous injection of the platelet factor 4 before injection of albino rats with tissue thromboplastin prevented the reaction of anticoagulation system inactivated the synthesis of endogenous thrombin. This effect is accompanied by high hypercoagulation and depression of nonenzymatic fibrinolysis in blood.

[Process of fibrin depolymerization and non-enzymatic fibrinolysis in rabbits receiving atherogenic rations with addition of antioxidants]. / Protsess depolimerizatsii fibrina i nefermentativnyi fibrinoliz u krolikov, poluchaiushchikh aterogennyi ratsion s dobavleniem antioksidantov.

The rabbits were kept on atherogenic ration for 2 months. This diet contained 0.3 mg/kg of cholesterol. In blood plasma of animals the authors observed a sharply reduced non-enzymatic fibrinolysis and depolymerization activity of non-stabilized fibrin. The addition of antioxidants and alpha-tocopherol (10 + 10 mg/kg) for 1 month to the atherogenic ration protected from the disturbance of the system hemostasis and normalized the depolymerization of non-stabilized fibrin.

[The content of a heparin-like anticoagulant in the flowers of the meadowsweet (Filipendula ulmaria)]. / Soderzhanie geparinopodobnogo antikoagulianta v tsvetkakh tavolgi viazolistnoi.

The flowers of Filipendula ulmaria were found to contain heparin bound to the plant proteins in the form of a complex. This complex enhances the anticoagulant and fibrinolytic properties of the nonenzymatic nature at its administration to animals both intramuscularly and intravenously. The neutralizing effect of protaminesulphate on the anticoagulant activity of the plant heparin was shown. The identity of the action on the hemostasis system of heparin of animal and plant origin was found.

[Platelet aggregation induced by fibrinmonomer and the effect of the heparin-adrenaline complex or plasmin on this process]. / Agregatsiia trombotsitov, vyzyvaemaia fibrin-monomerom, i vliianie kompleksnykh soedinenii geparina s adrenalinom ili plazminom na étot protsess.

Addition of purified fibrin-monomer in a concentration of 0.5 mg/ml induces aggregation in a suspension of washed rat platelets in the absence of aggregants. Maximum aggregation takes place 1-3 min after fibrin-monomer addition, and then the disaggregation phase follows. Complex heparin compounds with adrenalin or plasmin possessing antipolymerization and fibrinolytic activity with respect to ++non-stabilized fibrin prevent, or to a great extent decrease fibrin-monomer-induced aggregation of washed platelets.

[Heparin-histamine complex, its physico-chemical and biological properties]. / Kompleksnoe soedinenie geparina s gistaminom, ego fiziko-khimicheskie i biologicheskie svoistva.

Complexes between heparin and histamine at various ratio exhibited various physiological activity. The complex containing heparin-histamine at the ratio of 6:1, 10:1 or 15:1 showed anticoagulation, antipolymerization and nonenzymatic fibrinolytic effects. The complex dissociated in circulation within 90 min after its administration. This complex effected also the rheologic properties of blood.