[Immunoenzyme analysis and polymerase chain reaction in the diagnosis of an intrauterine infection in newborn infants with cerebral lesions]. / Immunofermentnyi analiz i polimeraznaia tsepnaia reaktsiia v diagnostike vnutriutrobnoi infektsii u novorozhdennykh s tserebral'nymi porazheniiami.

The comparative evaluation of the diagnostic value of the polymerase chain reaction (PCR) and the enzyme immunoassay (EIA) in the detection of intrauterine infection (IUI) in 48 newborn infants with cerebral lesions was made. Tests for the presence of the infective agents of IUI, most frequently occurring in the region (Cytomegalovirus, Herpes simplex virus, Chlamydia trachomatis), were carried out. The levels of serum IgA, IgG and IgM were evaluated in the course of the primary screening of IUI. Laboratory samples for PCR from infants with IUI were selected at random. The study demonstrated that in PCR the frequency of positive results was significantly greater than in EIA.

[Diagnostic criteria of pyloric helicobacter infection in peptic ulcer]. / Kriterii diagnostiki piloricheskogo khelikobakterioza pri iazvennoi bolezni.

Helicobacter pylori (HP) in 41 patients (10 with stomach ulcer and 31 with duodenum ulcer) was found in gastro-biopsies from the stomach antrum before and after the treatment. The following methods to reveal HP were used: Giemsa staining, immunoperoxidase reaction with polyclonal monospecific antibodies against HP, urease rapid test. HP was also revealed in the blood serum by antibodies against HP by means of enzyme immunoassay. Electron microscopy was performed in 5 patients and bacteriologic study in 10 patients. Immunohistochemical method allows one to judge about real HP colonization of the gastric mucosa. Bacterioscopy with Giemsa staining gives good results but they are not true markers of HP presence this being explained by other methods. Serologic enzyme immunoassay gives an idea about the patient contamination, but not about HP eliminations as a result of treatment. Urease-test is adjuvant.

[Comparative studies of immunologic characteristics in plasmapheresis and hemosorption in patients with purulent diseases of the lungs and pleura]. / Sravnitel'naia kharakteristika immunologicheskikh pokazatelei pri plazmafereze i gemosorbtsii u bol'nykh gnoinymi zaboleva- niiami legkikh i plevry.

The article deals with the comparative characteristics of the changes in immunological indices in 40 patients with non-specific purulent diseases of the lungs and pleura when plasmapheresis and hemosorption were included in the complex of therapeutic measures. The changes were found to be hetero-directional in character: a non-specific effect of immunostimulation in hemosorption, and immunodepression, particularly marked in the first 24 hours, in plasmapheresis. The post-aggressive reaction after plasmapheresis is either absent or delayed significantly, which makes it possible to conduct detoxification in extremely grave conditions of the patients without making the mechanisms of the post-aggressive reaction operative, as observed in performance of hemosorption.

[Circulating immune complexes and immunoglobulins A, M and G in the mother-fetus system in normal pregnancy and gestosis]. / Tsirkuliruiushchie immunnye kompleksy i immunoglobuliny A, M, G v sisteme mat'-plod pri fiziologicheskoi beremennosti i gestoze.

Circulating immune complexes (CIC) and immunoglobulins A, M, and G were measured in the maternal peripheral blood, mixed umbilical blood, and amniotic fluid in women with normal pregnancy and gestosis. CIC concentrations in the examined fluids did not surpass the levels typical of healthy donors. Maternal blood CIC levels were higher in gestosis than in normal pregnancy, and CIC levels in the umbilical blood and amniotic fluid were always lower than in maternal blood serum. IgG levels were found the most shifted. These data evidence changes of the humoral immunity in normal pregnancy, this being, probably, one of the mechanisms providing its favorable course.

[Immunological properties of the amniotic fluid in physiological pregnancy and gestoses]. / Immunologicheskie svoistva okoloplodnoi zhidkosti pri fiziologicheskoi beremennosti i gestozakh.

Immunologic analysis of the amniotic fluid included measurements of immunoglobulins A and M, circulating immune complexes, rheumatoid and antinuclear factors, immunophoresis and electrophoresis, and tests for the presence of donor lymphocytes, Changed immunologic parameters of the amniotic fluid indicate a complicated course of pregnancy, development of gestosis among other things. Basing on their findings, the authors suggest that gestosis involve dysfunction of the adaptive mechanisms, that may result from disordered interactions of the immunity system components.

[The immunomodulators levamisole and dibazole in the prevention of acute postoperative pleural empyema as one method of preoperative preparation]. / Immunomoduliatory levamizol i diabazol v profilaktike ostrykh posleoperatsionnykh émpiem plevry kak odin i metodov predoperatsionnoi podgotovki.

Numerous methodologies had been suggested to prevent postoperative pyothorax, but the tendency towards reduction in the incidence of this condition couldn't be seen. Forty patients with different lung diseases (malignant tumours, abscesses, bronchiectases) were examined. Twenty patients received low-dose levamisole and dibazole pre- and postoperatively, while to the remaining 20 patients these immunomodulators were not administered. All the 40 patients underwent radical operations. Immunoassay was undertaken in both groups in the preoperative period and at days 7, 14, and 21 postoperatively. As a result it was found that the group of patients devoid of immunoprophylaxis showed development of pyothorax in 5 percent of the cases. The groups of patients exposed to preventive measures including low doses of levamisole and dibazole did not show septic complications in the pleural cavity.