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1.
J Dermatolog Treat ; 35(1): 2373826, 2024 Dec.
Article in English | MEDLINE | ID: mdl-38964751

ABSTRACT

BACKGROUND: To evaluate the risk of neutropenia during treatment with anti-IL-23 antibodies in patients with psoriasis. METHOD: We conducted an observational study with cohort design using MID-NET® in Japan. We identified patients with psoriasis who were newly prescribed anti-IL-23 antibodies, anti-IL-17-antibodies, adalimumab, or apremilast between January 1, 2009, and March 31, 2021. We estimated the adjusted hazard ratio (aHR) of anti-IL-23 antibodies compared to that of anti-IL-17 antibodies, adalimumab, or apremilast, for the risk of grade 2 (neutrophil count < 1,500/µL) or grade 3 (neutrophil count < 1,000/µL) neutropenia. RESULTS: Overall, 287 patients on anti-IL-23 antibodies, 189 patients on anti-IL-17 antibodies, 293 patients on adalimumab, and 540 patients on apremilast were included. Compared with anti-IL-17 antibodies, the aHR (95% confidence interval (CI)) of anti-IL-23 antibodies was 0.83 (0.27-2.51) for grade 2 and 0.40 (0.02-7.60) for grade 3 neutropenia; that when compared with adalimumab was 0.76 (0.28-2.06) for grade 2 but was not calculated for grade 3 as no cases were found; and that compared with apremilast was 3.88 (0.62-24.48) for grade 2 and 0.43 (0.02-11.63) for grade 3 neutropenia. CONCLUSION: No clear increase in the risk of neutropenia with anti-IL-23 antibodies was observed.


Subject(s)
Adalimumab , Interleukin-17 , Interleukin-23 , Neutropenia , Psoriasis , Thalidomide , Humans , Adalimumab/adverse effects , Adalimumab/immunology , Psoriasis/drug therapy , Psoriasis/immunology , Female , Male , Neutropenia/chemically induced , Neutropenia/immunology , Neutropenia/epidemiology , Middle Aged , Japan , Adult , Interleukin-17/antagonists & inhibitors , Interleukin-17/immunology , Interleukin-23/antagonists & inhibitors , Interleukin-23/immunology , Thalidomide/adverse effects , Thalidomide/analogs & derivatives , Aged , Antibodies, Monoclonal, Humanized/adverse effects
3.
Biochem Biophys Res Commun ; 377(4): 1062-6, 2008 Dec 26.
Article in English | MEDLINE | ID: mdl-18992219

ABSTRACT

SNARK, a member of the AMPK-related kinases, has been involved in the cellular stress responses but its precise mechanisms remain unclear. Subcellular localization of SNARK protein was identified. Unlike cytoplasmic localizing AMPKalpha, SNARK was predominantly localized in the nucleus. SNARK was constitutively distributed in the nucleus even when SNARK was activated by metabolic stimuli such as AICAR and glucose-deprivation. Conserved nuclear localization signal (NLS) was identified at the N-terminal portion ((68)KKAR(71)). Deletion and point mutation of this part resulted in the cytoplasmic translocation of mutant proteins. Furthermore, GFP fused with the SNARK fragment containing (68)KKAR(71) translocated to the nucleus. A microarray analysis revealed that the nuclear localizing SNARK altered transcriptome profiles and a considerable part of these alterations were canceled by the mutation of NLS, suggesting the ability of SNARK to modulate gene expression dependent on its nuclear localization.


Subject(s)
Cell Nucleus/enzymology , Gene Expression Regulation , Nuclear Localization Signals/metabolism , Protein Serine-Threonine Kinases/metabolism , Active Transport, Cell Nucleus , Gene Expression Profiling , Green Fluorescent Proteins/genetics , Green Fluorescent Proteins/metabolism , HeLa Cells , Humans , Mutation , Nuclear Localization Signals/genetics , Protein Serine-Threonine Kinases/genetics
4.
Cancer Sci ; 99(4): 677-82, 2008 Apr.
Article in English | MEDLINE | ID: mdl-18307533

ABSTRACT

SNF-1/5'-AMP-activated kinase (AMPK)-related kinase (SNARK) is a member of the AMPK-related kinases. Snark(+/-) mice exhibited mature-onset obesity and related metabolic disorders. Obesity is regarded as a risk factor for colorectal cancer. To investigate whether Snark deficiency is involved in tumorigenesis in the large intestine, obese Snark(+/-) mice were treated with a chemical carcinogen, azoxymethane (AOM). The incidences of both adenomas and aberrant crypt foci (ACF) were significantly higher in Snark(+/-) mice than in their wild-type counterparts 28 weeks after the completion of AOM treatment (10 mg/kg/week for 8 weeks). Furthermore, ACF formation was enhanced in Snark(+/-) mice treated with AOM for 2 weeks, suggesting that Snark deficiency contributed to the early phase of tumorigenesis. The total number of ACF was correlated with bodyweight in Snark(+/-) and Snark(+/+) mice, suggesting that obesity was a risk factor for colorectal tumorigenesis in this model. However, the correlation coefficient was higher in Snark(+/-) mice. Moreover, AOM-induced ACF formation was also enhanced in preobese Snark(+/-) mice. Together, these findings suggest that AOM-induced tumorigenesis in Snark(+/-) mice was enhanced via obesity-dependent and -independent mechanisms.


Subject(s)
Colorectal Neoplasms/genetics , Genetic Predisposition to Disease , Obesity/genetics , Precancerous Conditions/genetics , Protein Serine-Threonine Kinases/genetics , Animals , Azoxymethane/toxicity , Body Weight , Carcinogens/toxicity , Colorectal Neoplasms/chemically induced , Colorectal Neoplasms/pathology , Female , Gastrointestinal Tract/enzymology , Gastrointestinal Tract/pathology , Mice , Mice, Mutant Strains , Obesity/complications , Precancerous Conditions/chemically induced , Precancerous Conditions/pathology , Risk Factors
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