ABSTRACT
Differences in RF values between D- and L-tryptophan on cellulose paper, developed with water, were observed already in 1954 and now several variables of this separation, such as modifications to the tryptophan molecule, the temperature and salting-out have been examined. Differences were found in the mechanism of the process compared with the systems described by Yuasa et al. [Chromatographia, 21 (1986) 79], who separated amino acid enantiomers on cellulose with pyridine-ethanol-water mixtures.
Subject(s)
Tryptophan/analogs & derivatives , Tryptophan/isolation & purification , Chemical Phenomena , Chemistry , Chromatography, Paper , SolventsABSTRACT
Separations of amines, phenols, alkaloids, etc., using cellulose papers or thin layers with aqueous ammonium sulphate as eluent are reported. These separations illustrate that excellent analytical separations are possible and provide data for the study of the mechanism of adsorption on cellulose from aqueous solutions.