Aerobic microbial Fe acquisition from ferrihydrite nanoparticles: effects of crystalline order, siderophores, and alginate.

This research compared the bioavailability of Fe associated with two forms of the hydrous Fe oxyhydroxide nanomineral ferrihydrite (Fh)--the smaller (1-3 nm), less ordered 2-line (2L) phase and the slightly larger, (2-6 nm) more ordered 6-line (6L) phase--to the common aerobic soil bacterium Pseudomonas mendocina ymp. P. mendocina can acquire Fe from minerals using high-affinity Fe(III) binding ligands known as siderophores and a cell-associated metalloreductase that requires direct cell-mineral contact. Wild-type (WT) P. mendocina and a siderophore(-) mutant were used to monitor siderophore -related and -independent Fe acquisition from 2L and 6L Fh. Both WT and mutant strains acquired Fe from Fh, although Fe acquisition and growth were substantially greater on the 2L phase than on the 6L phase. In the absence of bacteria, copious quantities of the biofilm exopolysaccharide alginate slightly promoted dissolution of 2L and 6L Fh. In biotic experiments, added alginate slightly enhanced growth and Fe acquisition from 6L Fh but not from 2L Fh. Recent research has led to an emerging understanding that Fe-oxide nanoparticle structure, stability, and reactivity are highly sensitive to size at the nanoscale; this research emphasizes how subtle differences in nanoparticle size-related properties can also affect bioavailability.

Volar A1 pulley approach for fixation of avulsion fractures of the base of the proximal phalanx.

Avulsion fractures of the base of the proximal phalanx associated with collateral ligament instability, excluding the thumb, are relatively rare. While the indications for surgical intervention vary, dorsal approaches have been advocated despite the volar location of the fracture fragment and orientation of the collateral ligaments. Ten patients with 11 avulsion fractures at the base of the proximal phalanx associated with collateral ligament instability were treated with open reduction and internal fixation using a volar A1 pulley approach. Anatomic restoration of the articular surface and collateral ligament stability were obtained in all patients. All fractures healed between 5 and 9 weeks (average, 6 weeks). After an average 19.4-month follow-up period all patients had full range of motion of the metacarpophalangeal joint, collateral ligament stability, and grip strength of at least 90% of the uninjured hand. No perioperative complications occurred. The average DASH score at last follow-up examination was 1.8 (range, 0-6). All patients were satisfied with the outcome of surgery. The volar A1 pulley approach is a direct and effective approach for reduction and fixation of avulsion fractures of the base of the proximal phalanx associated with collateral ligament instability.

Global and specific translational regulation in the genomic response of Saccharomyces cerevisiae to a rapid transfer from a fermentable to a nonfermentable carbon source.

The global gene expression program that accompanies the adaptation of Saccharomyces cerevisiae to an abrupt transfer from a fermentable to a nonfermentable carbon source was characterized by using a cDNA microarray to monitor the relative abundances and polysomal distributions of mRNAs. Features of the program included a transient reduction in global translational activity and a severe decrease in polysome size of transcripts encoding ribosomal proteins. While the overall translation initiation of newly synthesized and preexisting mRNAs was generally repressed after the carbon source shift, the mRNA encoded by YPL250C was an exception in that it selectively mobilized into polysomes, although its relative abundance remained unchanged. In addition, splicing of HAC1 transcripts, which has previously been reported to occur during accumulation of unfolded proteins in the endoplasmic reticulum, was observed after the carbon shift. This finding suggests that the nonconventional splicing complex, composed of the kinase-endonuclease Ire1p and the tRNA ligase Rlg1p, was activated. While spliced HAC1 transcripts mobilized into polysomes, the vast majority of unspliced HAC1 RNA accumulated in nonpolysomal fractions before and after the carbon source shift, indicating that translation of unspliced HAC1 RNA is blocked at the translation initiation step, in addition to the previously reported elongation step. These findings reveal that S. cerevisiae reacts to the carbon source shift with a remarkable variety of responses, including translational regulation of specific mRNAs and activation of specific enzymes involved in a nonconventional splicing mechanism.

The syndecan family of proteoglycans. Novel receptors mediating internalization of atherogenic lipoproteins in vitro.

Cell-surface heparan sulfate proteoglycans have been shown to participate in lipoprotein catabolism, but the roles of specific proteoglycan classes have not been examined previously. Here, we studied the involvement of the syndecan proteoglycan family. First, transfection of CHO cells with expression vectors for several syndecan core proteins produced parallel increases in the cell association and degradation of lipoproteins enriched in lipoprotein lipase, a heparan-binding protein. Second, a chimeric construct, FcR-Synd1, that consists of the ectodomain of the IgG Fc receptor Ia linked to the highly conserved transmembrane and cytoplasmic domains of syndecan-1 directly mediated efficient internalization, in a process triggered by ligand clustering. Third, internalization of lipase-enriched lipoproteins via syndecan-1 and of clustered IgGs via the chimera showed identical kinetics (t1/2 = 1 h) and identical dose-response sensitivities to cytochalasin B, which disrupts microfilaments, and to genistein, which inhibits tyrosine kinases. In contrast, internalization of the receptor-associated protein, which proceeds via coated pits, showed a t1/2 < 15 min, limited sensitivity to cytochalasin B, and complete insensitivity to genistein. Thus, syndecan proteoglycans can directly mediate ligand catabolism through a pathway with characteristics distinct from coated pits, and might act as receptors for atherogenic lipoproteins and other ligands in vivo.

Improving maternal and child outcomes of hard-to-reach populations.

One county health department has created an innovative program to provide pre- and postnatal health care to Hispanic women and children. This case study demonstrates the importance of finding new ways to meet the needs of a rapidly changing population, understanding different cultural beliefs, and establishing a level of trust to achieve positive outcomes within this hard-to-reach population.