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A rapid and simple method for detection of type II restriction endonucleases in cells of bacteria with high activity of nonspecific nucleases.
Podgórska, Beata; Kujawska, Grazyna; Skurzewski, Michal; Batsko, Olesya; Kaczorowski, Tadeusz.
Acta Biochim Pol ; 59(4): 669-72, 2012.
Article in English | MEDLINE | ID: mdl-23130356

ABSTRACT

In this work we describe a novel, rapid and simple microscale procedure for identification of restriction endonuclease activity in bacteria lysates, which contain high levels of non-specific DNA nucleases.


Subject(s)
Bacteria/enzymology , Deoxyribonucleases, Type II Site-Specific/isolation & purification , Deoxyribonucleases/chemistry , Deoxyribonucleases, Type II Site-Specific/chemistry , Substrate Specificity
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