Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 1 de 1
Filter
Add more filters










Database
Language
Publication year range
1.
Biomed Mater ; 14(6): 065005, 2019 09 09.
Article in English | MEDLINE | ID: mdl-31408854

ABSTRACT

The objective of this study was to develop a novel porous thin poly(D,L-lactide) (PLA) film as a tissue-engineering scaffold for keratinocytes used for the replacement of damaged skin. Poly(D,l-lactic acid)/poly(ethylene glycol) (PEG: Mw 6000 or 15 000) blend films were formed by a spin coating technique. The properties and structures of these blend films were investigated. PDLA/PEG (6000) blend films were modified by microfibrillar collagen after water incubation to increase hydrophilicity and improve keratinocyte adhesion. Primary keratinocytes were seeded on PLA films, cultivated for 9 d and transplanted to rats with a model skin defect wound. The wound's healing after keratinocyte transplantation was assayed with histological and immunochemical methods. It was found that skin damage recovery was the most effective after transplantation of keratinocytes on porous PLA film modified with collagen.


Subject(s)
Keratinocytes/cytology , Keratinocytes/drug effects , Polyesters/chemistry , Skin/growth & development , Tissue Engineering/methods , Tissue Scaffolds , Animals , Animals, Newborn , Biocompatible Materials/chemistry , Collagen/chemistry , Epidermis/metabolism , Female , Microscopy, Atomic Force , Polyethylene Glycols , Porosity , Rats , Rats, Wistar , Skin/cytology , Viscosity , Water , Wound Healing
SELECTION OF CITATIONS
SEARCH DETAIL
...