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1.
Stomatologiia (Mosk) ; 81(4): 24-8, 2002.
Article in Russian | MEDLINE | ID: mdl-12380292

ABSTRACT

The efficiency of combined treatment of dentures by argon plasma and chemical disinfectant Ocadez is compared using anaerobic culturing of oral bacteria and polymerase chain reaction with hepatitis B, C, and D virus primers. Different protocols of processing dental instruments and casts directly after dentist's manipulations with outpatients were tried and model experiments with hepatitis viruses were carried out. The optimal protocol of plasma sterilization is as follows: 10 min at 86-90 degrees C after treatment with Ocadez or 15 min without additional chemical treatment.


Subject(s)
Argon/therapeutic use , Benzalkonium Compounds/therapeutic use , Dental Disinfectants/therapeutic use , Sterilization/methods , Cross Infection/prevention & control , Dental Instruments/microbiology , Dental Instruments/virology , Hepatitis, Viral, Human/prevention & control , Multivariate Analysis , Outcome Assessment, Health Care
2.
Article in Russian | MEDLINE | ID: mdl-8701656

ABSTRACT

To study humoral response in patients with the generalized form of pseudotuberculosis, the agglutination test, the passive hemagglutination test and the complement fixation test with antigen obtained from "Virion" (Switzerland) were used. The study of the dynamics of antibody formation to outer membrane proteins (OMP) and Yersinia pseudotuberculosis (pYV+ and pYV-) lipopolysaccharide (LPS) revealed that the synthesis of antibodies to OMP, controlled by inv gene, exceeded the synthesis of antibodies to LPS at all periods of the disease, which determined the necessity of further study of the role of OMP in pathogenesis and in the formation of humoral response. On the basis of the study of the occurrence of antigens a high diagnostic effectiveness the enzyme immunoassay for the detection of Y. pseudotuberculosis antigens, mainly at the first stage of the disease, was noted.


Subject(s)
Yersinia pseudotuberculosis Infections/diagnosis , Acute Disease , Antibodies, Bacterial/blood , Antigens, Bacterial/blood , Dysentery, Bacillary/diagnosis , Evaluation Studies as Topic , Humans , Immunologic Tests/methods , Intestinal Diseases/diagnosis , Salmonella Infections/diagnosis , Time Factors , Yersinia pseudotuberculosis/immunology
4.
Zh Mikrobiol Epidemiol Immunobiol ; (7): 29-32, 1991 Jul.
Article in Russian | MEDLINE | ID: mdl-1950260

ABSTRACT

The results obtained in the study of antibiotic and sulfamide sensitivity of 197 Neisseria meningitidis strains of groups A, B and C, isolated from the spinal fluid and blood of patients with meningococcal infection hospitalized in the 2nd Clinico-Infectious Hospital, Moscow, in 1984-1989 and studied with the use of the disc diffusion method and the method of serial dilutions of antibiotics in solid culture media, are presented. As revealed in this study, N. meningitidis strains retained their high sensitivity to penicillin and ampicillin (MIC50 = 0.016 and 0.032 micrograms/ml respectively). Sensitivity to tetracycline decreased (MIC50 = 0.5 micrograms/ml) and to rifampicin increased (MIC50 = 0.063 micrograms/ml). 48.5% of strains were resistant to streptomycin. In recent years the proportion of N. meningitidis, resistant to sulfanilamide preparations, significantly decreased and MIC50 was equal to 2.5 micrograms/ml in comparison with 5-10 micrograms/ml in the preceding period. The results of testing sensitivity to antibiotics by both methods coincided. Still the disc diffusion method can be used in epidemiological surveillance on meningococcal infection, while for more exact differentiation of N. meningitidis strains the use of the method of serial dilutions is necessary.


Subject(s)
Neisseria meningitidis/drug effects , Anti-Bacterial Agents/pharmacology , Drug Resistance, Microbial , Evaluation Studies as Topic , Humans , Microbial Sensitivity Tests/instrumentation , Microbial Sensitivity Tests/methods , Microbial Sensitivity Tests/statistics & numerical data , Neisseria meningitidis/classification , Neisseria meningitidis/isolation & purification , Regression Analysis , Serotyping , Staphylococcus aureus/drug effects , Sulfanilamides/pharmacology
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