Nitrogen doped carbon dots and gold nanoparticles mediated FRET for the detection of creatinine in human urine samples.

Serum creatinine (CR) is regarded as one of the most sought out prognostic biomarkers in medical evaluation of chronic kidney disease (CKD). In light of the diagnostic significance of CR, the utility of a fluorescence biosensor for its detection in human urine specimens has been explored based on Förster resonance energy transfer (FRET) across nitrogen-doped carbon dots (N-CDs) and gold nanoparticles (GNPs). A straightforward microwave-assisted synthesis procedure has been adopted to prepare N-CDs (λexcitation = 400 nm, λemission = 540 ± 5 nm) with bright green emissions. On addition of pre-synthesized GNPs, the radiative emanation of the N-CDs is completely suppressed on account of FRET across the N-CDs and the GNPs. About 77 % of their fluorescence intensity is recovered after adding CR to GNPs@N-CDs nanocomposite. The limit of detection for CR sensing is estimated as 0.02 µg•mL-1. This biosensor is selective enough to recognize CR in the existence of potential interfering substances (e.g., ascorbic acid, glucose, glutathione, urea, and electrolytes). Its practical utility for CR detection has been validated further on the basis of satisfactory correlation with the benchmark Jaffe method, as observed in artificial/human urine specimens. Consequently, this manuscript marks a pioneering report on employing CDs and GNPs-based FRET for identifying CR in urine specimens of CKD patients.

A comprehensive review on immunogen and immune-response proteins of SARS-CoV-2 and their applications in prevention, diagnosis, and treatment of COVID-19.

Exposure to severe acute respiratory syndrome-corona virus-2 (SARS-CoV-2) prompts humoral immune responses in the human body. As the auxiliary diagnosis of a current infection, the existence of viral proteins can be checked from specific antibodies (Abs) induced by immunogenic viral proteins. For people with a weakened immune system, Ab treatment can help neutralize viral antigens to resist and treat the disease. On the other hand, highly immunogenic viral proteins can serve as effective markers for detecting prior infections. Additionally, the identification of viral particles or the presence of antibodies may help establish an immune defense against the virus. These immunogenic proteins rather than SARS-CoV-2 can be given to uninfected people as a vaccination to improve their coping ability against COVID-19 through the generation of memory plasma cells. In this work, we review immunogenic and immune-response proteins derived from SARS-CoV-2 with regard to their classification, origin, and diverse applications (e.g., prevention (vaccine development), diagnostic testing, and treatment (via neutralizing Abs)). Finally, advanced immunization strategies against COVID-19 are discussed along with the contemporary circumstances and future challenges.

Facile adsorption of organophosphate pesticides over HKUST-1 MOFs.

The recovery of organophosphate pesticides (OPPs) from aqueous solutions is imperative considering their agricultural and environmental implications. Among various mitigation approaches used for OPPs' removal, adsorption offers many advantageous features for OPPs abatement owing to its benign nature, cost-effective processing, and non-requirement of excessive equipment. This research describes the adsorptive removal of three organophosphate pesticides (OPPs) namely chlorpyrifos (CPF), methyl parathion (MP), and malathion (MAL) by HKUST-1 (HKUST = Hong Kong University of Science and Technology) metal-organic framework (MOF). The synthesis of HKUST-1 MOFs was confirmed by various spectroscopic and microscopic techniques. The adsorption kinetics was systematically investigated by varying three parameters to include solution pH, contact time, and initial pesticide concentration. Among all the three pesticides, HKUST-1 showed enhanced removal of CPF in terms of pH, resulting in an adsorption capacity of 1.82 mg·g-1. However, under the effect of contact time at 60 min, the adsorption capacity of HKUST-1 for PM, MAL, and CPF were computed to be 1.83, 1.79, and 0.44 mg·g-1, respectively. Besides, HKUST-1 showed a remarkable performance towards adsorptive removal of MAL (14.01 mg·g-1 at 10 mg·L-1 concentration) with linear increase in adsorption capacity as the function of initial pesticide concentration. The MOFs were also able to retain ca. 50% of their adsorption efficiency over the course of five cycles of adsorptive removal of CP. In the future, a comprehensive data table showing the performance of various MOFs against various OPPs can be constructed on the basis of parameters used in this study.

Recent advances in the synthesis and applications of single-atom nanozymes in food safety monitoring.

Nanozymes are synthetic compounds with enzyme-like tunable catalytic properties. The success of nanozymes for catalytic applications can be attributed to their small dimensions, cost-effective synthesis, appreciable stability, and scalability to molecular dimensions. The emergence of single atom nanozymes (SANzymes) has opened up new possibilities in bioanalytical applications. In this regard, this review outlines enzyme-mimicking features of SANzymes for food safety applications in relation to the key variables controlling their catalytic performance. The discussion is extended further to cover the applications of SANzymes for the monitoring of various compounds/biomaterials of significance with respect to food safety (e.g., pesticides, veterinary drug residues, foodborne pathogenic bacteria, mycotoxins/bacterial endotoxin, antioxidant residues, hydrogen peroxide residues, and heavy metal ions). Furthermore, the performance of SANzymes is evaluated in terms of various performance metrics such as limit of detection (LOD), linear dynamic range, and figure of merit (FoM). The challenges and future road map for the applications of SANzymes are also addressed along with their upscaling in the area of food safety.

"Lock-and-key" recognizer-encoded lateral flow assays toward foodborne pathogen detection: An overview of their fundamentals and recent advances.

In light of severe health risks of foodborne pathogenic bacterial diseases, the potential utility of point-of-care (POC) sensors is recognized for pathogens detection. In this regard, lateral flow assay (LFA) is a promising and user-friendly option for such application among various technological approaches. This article presents a comprehensive review of "lock-and-key" recognizer-encoded LFAs with respect to their working principles and detection performance against foodborne pathogenic bacteria. For this purpose, we describe various strategies for bacteria recognition including the antibody-based antigen-antibody interactions, nucleic acid aptamer-based recognition, and phage-mediated targeting of bacterial cells. In addition, we also outline the technological challenges along with the prospects for the future development of LFA in food analysis. The LFA devices built based upon many recognition strategies are found to have great potential for rapid, convenient, and effective POC detection of pathogens in complex food matrixes. Future developments in this field should emphasize the development of high-quality bio-probes, multiplex sensors, and intelligent portable readers.

Application of SERS-based nanobiosensors to metabolite biomarkers of CKD.

A clinical diagnosis of chronic kidney disease (CKD) is commonly achieved by estimating the serum levels of urea and creatinine (CR). Given the limitations of the conventional diagnostic assays, it is imperative to seek alternative, economical strategies for the detection of CKD-specific biomarkers with high specificity and selectivity. In this respect, surface-enhanced Raman spectroscopy (SERS) can be regarded as an ideal choice. SERS signals can be greatly amplified by noble metal nanoparticles (e.g., gold nanoparticles (GNPs)) of numerous sizes, shapes, and configurations to help achieve ultra-sensitive single molecule-level detection at 10-15 M (up to 10 orders of magnitude more sensitive than fluorescence-based detection). The irregular geometry of GNPs with spike-like tips, dimers, and aggregates with small nanogaps (i.e., due to plasmon coupling such as Raman hot spots) play a pivotal role in enhancing the specificity and sensitivity of SERS. This review critically outlines the performance of SERS-based biosensors in the ultrasensitive detection of CKD biomarkers in various body fluids in terms of basic quality assurance parameters (e.g., limit of detection, figure of merit, enhancement factor, and stability of the biosensor). Moreover, the challenges and perspectives are described with respect to the expansion of such sensing techniques in practical clinical settings.

Global distribution of microplastic contaminants in aquatic environments and their remediation strategies.

This review describes the occurrence and distribution of microplastics in freshwater and marine environments in recent years (2017-2022). Use of microplastics often results in contamination of aquatic environments, threatens biodiversity, and creates the need for environmental remediation. Such remediation strategies can involve primary, secondary, and tertiary treatments. Tertiary treatment is a frequent research subject due to its high efficiency and the possibility for advancements and enhancements. This study discusses tertiary treatments with remediation efficiencies of 95% and greater and their advantages, disadvantages, and future perspectives. Biochar-mediated remediation of microplastics is an effective method that may be able to achieve efficiencies approaching 100%. The study concludes by exploring methods of removing microplastics, including constructed wetlands and biochar, which offer high efficiency. PRACTITIONER POINTS: Tertiary treatments are an effective microplastic remediation strategy applicable succeeding secondary or primary treatments or as an individual remediation strategy. Biochar is a highly efficient adsorbent for microplastic remediation from aquatic environment with eco-friendly aspect and reusability. Modifications in tertiary treatments and enhancement in remediation efficiency are still a subject of research for future studies.

Recent advances in metagenomic analysis of different ecological niches for enhanced biodegradation of recalcitrant lignocellulosic biomass.

Lignocellulose wastes stemming from agricultural residues can offer an excellent opportunity as alternative energy solutions in addition to fossil fuels. Besides, the unrestrained burning of agricultural residues can lead to the destruction of the soil microflora and associated soil sterilization. However, the difficulties associated with the biodegradation of lignocellulose biomasses remain as a formidable challenge for their sustainable management. In this respect, metagenomics can be used as an effective option to resolve such dilemma because of its potential as the next generation sequencing technology and bioinformatics tools to harness novel microbial consortia from diverse environments (e.g., soil, alpine forests, and hypersaline/acidic/hot sulfur springs). In light of the challenges associated with the bulk-scale biodegradation of lignocellulose-rich agricultural residues, this review is organized to help delineate the fundamental aspects of metagenomics towards the assessment of the microbial consortia and novel molecules (such as biocatalysts) which are otherwise unidentifiable by conventional laboratory culturing techniques. The discussion is extended further to highlight the recent advancements (e.g., from 2011 to 2022) in metagenomic approaches for the isolation and purification of lignocellulolytic microbes from different ecosystems along with the technical challenges and prospects associated with their wide implementation and scale-up. This review should thus be one of the first comprehensive reports on the metagenomics-based analysis of different environmental samples for the isolation and purification of lignocellulose degrading enzymes.

Photocatalytic degradation of gaseous benzene using metal oxide nanocomposites.

Rapid industrial growth has been accompanied by the pollution of hazardous volatile organic pollutants (VOCs) in air. Among various options available for the treatment of VOCs, the use of metal oxide composites as photocatalysts has been adopted preferably due to their potential to induce the synergistic interactions between the metal nanoparticles (NPs) and metal oxides (especially titanium dioxide (TiO2)). In this context, an in-depth review is offered to describe the fundamental mechanism of metal oxide-based photocatalysis for the oxidation of gaseous benzene as a model VOC. The discussion has been extended further to evaluate their performances in terms of key performance metrics (e.g., quantum yield (QY), space-time yield (STY), and figure of merit (FOM)). The TiO2-based metallic bi-component photocatalysts (e.g., Sr2CeO4/TiO2) generally exhibited better photodegradation efficiency with enhanced light absorption capability than monometallic-TiO2 (e.g., Pd-TiO2) composites or other modified photocatalysts (e.g., metal-organic framework (MOF)-based composites). Finally, we address the current challenges and future perspectives in this highly challenging research field.

Volatile organic compounds released in the mainstream smoke of flavor capsule cigarettes.

In this study, the composition of mainstream smoke was investigated with an emphasis on a list of volatile organic compounds (VOCs: e.g., isoprene, acrylonitrile, methyl ethyl ketone, benzene, toluene, m-xylene and styrene) using the two types of flavor capsule cigarettes (FCCs, here coded as F1 and F2) in reference to one commercial, non-flavored (NF) and 3R4F cigarette. The concentrations of all the target compounds from FCCs were quantified under two contrasting conditions (i.e., with and without breaking the capsules). The effect of breaking the capsule was apparent in the FCC products with the enhancement of VOC levels, specifically between after and before breaking the capsules (e.g., 1.10-1.58 folds (benzene) and 1.30-1.53 folds (acetonitrile)). Such increases were apparent in both FCC samples if assessed in terms of the total amount of VOCs (TVOC): (1) F1 (from 2159 to 2530 µg cig-1 (p = 9.42 × 10-6)) and (2) F2 (from 1470 to 2014 µg cig-1 (p = 0.05)). In addition, these TVOC levels determined from the FCCs were 1.62- to 1.83- and 1.29- to 1.46-fold higher than those of the NF cigarette and the 3R4F cigarette, respectively. Thus, these FCC products are suspected to play a role as stronger sources of VOCs than the general cigarette products.

Advances in surface plasmon resonance-based biosensor technologies for cancer biomarker detection.

Surface plasmon resonance approach is a highly useful option to offer optical and label-free detection of target bioanalytes with numerous advantages (e.g., low-cost fabrication, appreciable sensitivity, label-free detection, and outstanding accuracy). As such, it allows early diagnosis of cancer biomarkers to monitor tumor progression and to prevent the recurrence of oncogenic tumors. This work highlights the recent progress in SPR biosensing technology for the diagnosis of various cancer types (e.g., lung, breast, prostate, and ovarian). Further, the performance of various SPR biosensors is also evaluated in terms of the basic quality assurance criteria (e.g., limit of detection (LOD), selectivity, sensor response time, and reusability). Finally, the limitations and future challenges associated with SPR biosensors are also discussed with respect to cancer biomarker detection.

Chitosan-Ni/Fe layered double hydroxide composites as an efficient solid phase extraction sorbent for HPLC-PDA monitoring of parabens in personal care products.

There is a dire need for development of efficient and sensitive methods to efficiently screen parabens. In this research, we focused on quantification of four parabens (i.e., methylparaben (MP), ethylparaben (EP), propylparaben (PP), and butyl paraben (BP)) using chitosan intercalated nickel/iron layered double hydroxide (CS-Ni/Fe-LDH) composites as solid phase extraction sorbent prior to HPLC-PDA analysis. CS-Ni/Fe LDH composites with a heterogeneous, porous texture, and coral reef-like structure exhibit appealing extraction efficiency for the target parabens due to the enhanced possibility for the formation of hydrogen bonding and hydrophobic interactions. The performance of the composites was assessed and optimized for solid phase extraction of parabens from standard samples and real samples (rose water, cream, toothpaste, hair serum, and sunscreen). The LDH-SPE-HPLC method exhibited a wide linear range (e.g., 100-50,000 ng L-1), good linearity (R2 ≥ 0.999), and good precision (relative standard deviation (RSD) < 3%). This method successfully enriched selected parabens with remarkable recovery above 85.95% and a good RSD (0.01-2.90%). The quantitation of MP, EP, PP, and BP was made at detection range (and limits of detection (LOD)) of 5-15 (9.8), 11-21 (16.2), 6-18 (12.4), and 10-20 (15.6) ng L-1, respectively. The prepared composites also displayed excellent performance with enhanced reusability/durability (n = 30 cycles) and reproducibility (n = 5).

Recent advances in nanoscale materials for antibody-based cancer theranostics.

The quest for advanced management tools or options of various cancers has been on the rise to efficiently reduce their risks of mortality without the demerits of conventional treatments (e.g., undesirable side effects of the medications on non-target tissues, non-targeted distribution, slow clearance of the administered drugs, and the development of drug resistance over the duration of therapy). In this context, nanomaterials-antibody conjugates can offer numerous advantages in the development of cancer theranostics over conventional delivery systems (e.g., highly specific and enhanced biodistribution of the drug in targeted tissues, prolonged systemic circulation, low toxicity, and minimally invasive molecular imaging). This review comprehensively discusses and evaluates recent advances in the application of nanomaterial-antibody bioconjugates for cancer theranostics for the further advancement in the control of diverse cancerous diseases. Further, discussion is expanded to cover the various challenges and limitations associated with the design and development of nanomaterial-antibody conjugates applicable towards better management of cancer.

Recent Advances in Monitoring, Sampling, and Sensing Techniques for Bioaerosols in the Atmosphere.

Bioaerosols in the form of microscopic airborne particles pose pervasive risks to humans and livestock. As either fully active components (e.g., viruses, bacteria, and fungi) or as whole or part of inactive fragments, they are among the least investigated pollutants in nature. Their identification and quantification are essential to addressing related dangers and to establishing proper exposure thresholds. However, difficulties in the development (and selection) of detection techniques and an associated lack of standardized procedures make the sensing of bioaerosols challenging. Through a comprehensive literature search, this review examines the mechanisms of conventional and advanced bioaerosol detection methods. It also provides a roadmap for future research and development in the selection of suitable methodologies for bioaerosol detection. The development of sample collection and sensing technology make it possible for continuous and automated operation. However, intensive efforts should be put to overcome the limitations of current technology as most of the currently available options tend to suffer from lengthy sample acquisition times and/or nonspecificity of probe material.

Recent advances in carbon nanotube sponge-based sorption technologies for mitigation of marine oil spills.

HYPOTHESIS: Oil spills stemming from supertankers, drilling, and natural events represent a serious problem worldwide due to the potential harms to marine ecosystems and aquatic life. To date, various functional absorbents have been developed to treat spilled oil. Among them, carbon nanotube (CNT)-based aerogels and sponges gained attention due to superior performance in uptake and recovery of various types of oil and organic solvents. CNT aerogel/sponge absorbents are demonstrated for a multitude of merits such as: rapid superhydrophobic/superoleophilic absorption (water contact angle > 150°), high capacity (≥100 mg g-1), large surface area (300-400 m2 g-1)), enhanced strength and flexibility (>95% volume reduction and restoration of pristine morphology at <0.25 MPa stress), mesoporous characteristics with high pore density (pore diameter = 80 nm and >99% porosity), recyclability, and easy surface modification. EXPERIMENTS: This review compares CNT sponge-based absorbents with conventional techniques for remediation/recovery of spilled oil. Typically, synthesis of CNT sponges is performed using chemical vapor deposition (CVD) approach in the presence of a catalyst or using sacrificial removal of template. This work summarizes recent progress in strategies for oil-spill treatment based on CNT sponge techniques. The performance of CNT sponges for oil spill removal was evaluated in terms of their adsorption capacity, compressive stressability, and desorption methods (e.g., heat treatment, burning, or squeezing). FINDINGS: CNT sponges were observed to have high performance for removal of oil spills in terms of key performance metrics. This review offers valuable insights into the current state of CNT-mediated oil-spill cleanup technologies and guidance for future research at the same time. This literature survey would help the stakeholders (researchers, scientists, entrepreneurs, and commercial houses) pursue contamination-free water.

Potential applications of graphene-based nanomaterials as adsorbent for removal of volatile organic compounds.

In recent years, graphene-based materials (GBMs) have been regarded as the core technology in diverse research fields. Consequently, the demand for large-scale synthesis of GBMs has been increasing continuously for various fields of industry. These materials have become a competitive adsorbent for the removal of environmental pollutants with improved adsorption capacity and cost effectiveness through hybridization or fabrication of various functionalities on their large surface. In particular, their applicability opens up new avenues for the adsorptive removal of volatile organic compounds (VOCs) (e.g., through the build-up of efficient air purification systems). This review explored the basic knowledge and synthesis approaches for GBMs and their performances as adsorbent for VOC removal. Moreover, the mechanisms associated with the VOC removal were explained in detail. The performance of GBMs has also been evaluated along with their present limitations and future perspectives.

Amine-Functionalized Metal-Organic Frameworks and Covalent Organic Polymers as Potential Sorbents for Removal of Formaldehyde in Aqueous Phase: Experimental Versus Theoretical Study.

Porous materials have been identified as efficient sorbent media to remove volatile organic compounds. To evaluate their potential as adsorbents, the adsorptive removal of formaldehyde (FA) in aqueous environments was investigated using four materials, two water-stable metal-organic frameworks (MOFs) of UiO-66 (U6) and U6-NH2 (U6N) and two covalent organic polymers (COPs) with amine-functionality, CBAP-1-EDA (CE) and CBAP-1-DETA (CD). U6N exhibited the highest removal capacity of 93% (0.56 mg g-1) of the tested materials [e.g., CE (81.1%, 0.53 mg g-1) > CD (67.2%, 0.43 mg g-1) > U6 (66.9%, 0.42 mg g-1)], which was 2 times higher than that of the reference sorbent, activated carbon (AC: 50%, 0.30 mg g-1). The results of Fourier transform infrared and powder X-ray diffraction analyses confirmed the interactions between FA molecules and the amine components of the materials (U6N, CD, and CE). According to density functional theory calculations, the formation of hydrogen bonds between FA molecules and amine components was apparent and was further verified by FA/amine distance (CD: 2.83, CE: 2.88, and U6N: 2.66 Å) along with enthalpy values (CD: -32.4, CE: -45.5, and U6N: -272 kJ mol-1). In case of U6, the major interactions occurred in the metal-clusters (-19.3 kJ mol-1) via electrostatic interactions (distance: 5.49 Å). Furthermore, the sorption by amine-functionalized materials such as U6N is suggested to be dominated by hydrogen bonding which ultimately led to the formation of imine. If the performance of the tested materials is evaluated in terms of partition coefficient, U6N (1153 mg g-1 mM-1) is found as the outperformer in all tested subjects. Regeneration of spent MOFs/COPs was also plausible in the presence of ethanol to maintain their structural integrity even after 10 adsorption-desorption cycles. Overall, the selected MOFs/COPs were seen to have very high removal capacity for hazardous FA molecules in aqueous phase.

Potential use of polymers and their complexes as media for storage and delivery of fragrances.

The use of fragrances is often essential to create an elegant, welcoming, or exhilarating environment. Through encapsulation, the release and delivery of fragrances are customized in many consumer products. For such purposes, cost-effective techniques have been developed and employed with the use of various polymers and porous organic materials to efficiently impart fragrances to foods and various other consumer products. After entrapment or uptake/storage of fragrant molecules within a polymeric complex, the properties can be investigated by automated thermal desorption (ATD) analysis. For efficient delivery, fragrances are adsorbed (or entrapped) in some media (e.g., fabric or paper). The release of such entrapped fragrances usually is achieved by spraying. Fragrances can be also loaded in a media by purging aroma gases or by adding fragrance essence directly into a liquid medium. Porous materials, such as zeolites, have been traditionally used for air purification as well as in cosmetics and similar applications. Similarly, other polymeric porous complexes have also been used in fragrance delivery as a templating agent for aromatherapy textiles. Such polymeric materials offer an advantage in terms of development of new hybrid blends via homogenous mixing of two or more matrices. Such blends may possess different desirable physical properties as encapsulants. This review article is aimed at presenting an overview of polymers and their complexes as the main media of fragrance encapsulation. This study also discusses the expansion and future application of porous materials as host matrices for fragrances.

Potential Utility of Metal-Organic Framework-Based Platform for Sensing Pesticides.

The progress in modern agricultural practices could not have been realized without the large-scale contribution of assorted pesticides (e.g., organophosphates and nonorganophosphates). Precise tracking of these chemicals has become very important for safeguarding the environment and food resources owing to their very high toxicity. Hence, the development of sensitive and convenient sensors for the on-site detection of pesticides is imperative to overcome practical limitations encountered in conventional methodologies, which require skilled manpower at the expense of high cost and low portability. In this regard, the role of novel, advanced functional materials such as metal-organic frameworks (MOFs) has drawn great interest as an alternative for conventional sensory systems because of their numerous advantages over other nanomaterials. This review was organized to address the recent advances in applications of MOFs for sensing various pesticides because of their tailorable optical and electrical characteristics. It also provides in-depth comparison of the performance of MOFs with other nanomaterial sensing platforms. Further, we discuss the present challenges (e.g., potential bias due to instability under certain conditions, variations in the diffusion rate of the pesticide, chemical interferences, and the precise measurement of luminesce quenching) in developing robust and sensitive sensors by using tailored porosity, functionalities, and better framework stability.

Transportation of drug-(polystyrene bead) conjugate by actomyosin motor system.

The Nanorobotics and cargo transportation application of molecular motors is of recent intent. The present study explores the transportation of Mesalamine/5-aminosalicylic acid/5-ASA drug by molecular motors. Mesalamine is an anti-inflammatory drug used to treat Crohn's disease and ulcerative colitis. Conjugate of mesalamine and polystyrene (Dia.: 3 microm) beads was prepared by amide linkage between amine (-NH2) group of drug and carboxyl (-COOH) group of the bead. In Fourier Transform Infrared spectra, peaks were observed at 3428.1 and 1654.0 cm(-1) for N-H and C=O stretching bond respectively confirming the amide bond formation between drug and microbeads. Quantification of 5-ASA attached to polystyrene bead was done by UV-vis spectroscopy and it was ascertained that 93% of 5-ASA was loaded on polystyrene beads. Conjugate of drug-polystyrene beads were then covalently attached to actin filaments. Velocity of actin filaments attached to drug loaded beads in in-vitro motility assay reduced to 0.89 microm/s as compared to free actin velocity (4.64 microm/s). This further ascertains the microcomposites formation. The present study provides an insight into the actin-myosin based molecular motor systems for an efficient tool for drug transportation.