ABSTRACT
A method for concentration of nucleoside triphosphates (NTP) is described. NTPs are quantitatively coprecipitated from the solution with calcium fluoride. The precipitate is separated by filtration through a membrane filter and NTPs are dissolved from the filter by immersing it in 0.5 N H2SO4. With this method also nucleoside diphosphates can be efficiently concentrated, but the method does not work with nucleoside monophosphates or cyclic AMP.
Subject(s)
Calcium Fluoride , Fluorides , Nucleotides/analysis , Potassium Compounds , Adenosine Monophosphate/analysis , Adenosine Triphosphate/analysis , Chemical Phenomena , Chemical Precipitation , Chemistry , Escherichia coli/analysis , PotassiumABSTRACT
The relative rate of DNA synthesis increases over control level and the synthesis of inorganic pyrophosphatase is stimulated when cells of E. coli are cultured in the presence of penicillins which inhibit cell division and cause filamentation of the cells. In contrast to that in temperature sensitive cell division mutants filamentation at 42 degrees C does not change the DNA/cell mass ratio and the synthesis of inorganic pyrophosphatase is not stimulated. This suggests that the change in the synthesis of the enzyme is not correlated with the filamentation but is a consequence of the supernormal DNA/cell mass ratio that appears in the presence of penicillins.