Subject(s)
Bone Marrow Cells , Hematopoietic Stem Cells/cytology , Adult , Cadaver , Cell Separation/methods , Humans , Male , Middle Aged , Specimen Handling/methods , Time FactorsSubject(s)
Corneal Opacity/surgery , Corneal Transplantation , Adolescent , Adult , Aged , Female , Freeze Drying , Humans , Male , Middle Aged , Organ PreservationABSTRACT
Two methods of preparing the pig-skin at a meat-packing plant and its application as a biological bandage in the clinic of thermal injuries are described. The first method is the sterile preparing. The second one--xenotransplants are prepared without an observation of aseptics rules. The material is subject to lyophilic drying and sterilization by gamma rays. The application of xenotransplants gave good results in the treatment of 107 patients with burns.
Subject(s)
Bandages , Biological Dressings , Burns/therapy , Animals , Body Surface Area , Freeze Drying , Humans , Skin , Sterilization , SwineSubject(s)
Burns/surgery , Skin Transplantation , Transplantation, Heterologous , Animals , Debridement/methods , Humans , Laser Therapy , Necrosis , Swine , Transplantation, AutologousSubject(s)
Cornea/ultrastructure , Tissue Preservation/methods , Cell Survival , Freeze Drying , Freezing , Humans , Microscopy, Electron , Time FactorsABSTRACT
Spleen cells of subjects who died suddenly are capable of producing large amounts of interferon in response to induction with Newcastle disease virus (NDV) and Sendai virus (1-2.5 IU per 1000 cells). The optimal conditions for interferon production by these cells include using NDV, the H strain, as an inducer in a dose of 10 ID50/cell and fresh spleen cells stored no more than 24 hours at 4-6 degrees C in a concentration of 0.5-1.0 x 10(7)/ml, with the incubation time of 20-24 hours. It is recommended that spleen cells from subjects who died suddenly be used for human interferon production.
