ABSTRACT
Mixed salivary pools of normal subjects and patients with galvanism, yeast-induced stomatitis, and diabetes mellitus were examined. The examinations have revealed elevated alpha-amylase levels in the patients with galvanism and still higher levels of this enzyme in yeast-induced stomatitis and diabetes mellitus. These diseases are also associated with a rise of lactoferrin content and with appearance of fibrinogen degradation products.
Subject(s)
Candidiasis, Oral/metabolism , Electrogalvanism, Intraoral , Salivary Proteins and Peptides/analysis , Adult , Diabetes Mellitus/metabolism , Humans , Paresthesia/metabolismSubject(s)
Antigens, Neoplasm/analysis , Carcinoembryonic Antigen/analysis , Pancreatic Neoplasms/diagnosis , Animals , Chromatography, Gel , Cytoplasm/analysis , Glycoproteins/blood , Humans , Immunoelectrophoresis , Immunoenzyme Techniques , Lung Neoplasms/diagnosis , Mice , Pancreatic Juice/analysis , Pancreatic Neoplasms/immunology , Pancreatitis/diagnosis , Rabbits , Radioimmunoassay , Stomach Neoplasms/diagnosis , alpha-Fetoproteins/analysisABSTRACT
The extraction of acetone-dried bacteria by means of tris-glycine buffer has been found to yield the greatest number of antigens. Thus, up to 16 antigenic components with different electrophoretic mobility have been revealed in Y. enterocolitica. They include 4 thermostable proteins and 2 glycoproteins. Such antigens may be common and specific for each serovar. The presence of 1-3 surface antigens in common with the causative agent of plague has been revealed. Of these, the most active antigen has proteinaceous nature, and its mobility corresponds to that of rho-globulin. It is not identical with the surface Y. pestis antigen located in the albumin zone.
Subject(s)
Antigens, Bacterial/analysis , Antigens, Surface/analysis , Yersinia/immunology , Animals , Immunization , Immunochemistry , Immunodiffusion , Immunoelectrophoresis , Rabbits , SolubilityABSTRACT
Studies have been made on water soluble antigens of the retina from man and some animals. In the bovine retina, immunochemical analysis reveals, apart from antigens with a broad and narrow interorganic specificity, organospecific alpha 1- and rho-globulins. Immunochemically, the bovine alpha 1-globulin is partially identical with the same protein of the human retina and completely identical to retinal antigens from cattle; rho-globulin is characterized as an interspecific antigen in man and mammals. Molecules of organospecific alpha 1-globulins from the retina of man and some animals (sheep, camel, horse, cow, pig) do not contain the determinants related to the retinal antigens from fishes, reptiles and birds. In human and mammalian retina, acid neurospecific alpha 1-glycoprotein was found which is topical of the cerebral tissue. Organospecific alpha 1-globulin of the bovine retina is located in the pigment epithelium, in the zone of outer and inner photoreceptor segments; organospecific rho-globulin is distributed in the outer synaptic layer of the retina.
Subject(s)
Eye Proteins/analysis , Retina/analysis , Animals , Antigens/analysis , Biological Evolution , Camelus/immunology , Cattle , Epitopes/analysis , Horses/immunology , Humans , Male , Organ Specificity , Rabbits , Sheep/immunology , Solubility , Swine/immunologyABSTRACT
A method of growing Bdellovibrio bacteriovorus (Bdv) based on the use of the host microbe in the state of reduced vital activity permitted to obtain Bdv cultures which could be used for the preparation specific antisera. Immunochemical analysis of 4 Bdv strains showed them to possess individual antigenic components localized in the alpha 2-globulin zone. Testing 17 Bdv strains isolated from natural water bodies showed 11 of them to form precipitation lines with the antisera to 4 Bdv strains.
Subject(s)
Antigens, Bacterial , Bdellovibrio/immunology , Animals , Immunodiffusion , Immunoelectrophoresis , Rabbits/immunologyABSTRACT
The effect of properdin, lysozyme and complementary activity of the blood serum on the degree of immune alteration of leukocytes was revealed on the clinico-experimental material. In the leukocytolysis a neutrophil apparently serves as a mechanical substrate. It is likely that the cytophilic organic antibodies conditioned the indirect variety of leukocytolysis.