Changing of the dynamics modes in populations with age and sex structure.

Using a mathematical model of the number dynamics in a population with age and sex structure, the relationships were studied between the number of newborns and that of mature females and males. In addition, we studied transitions between different modes of population dynamics which were dependent on changes in not only the population parameters, such as reproduction intensity, survival, and self-regulation, but also in mating pair formation. Population dynamics was found to manifest multistability; i.e., the alternative dynamics modes were observed at the same values of the population parameters but under different initial conditions.

[The comparative evalution of informativeness of immunologic and molecular genetic methods and means during stages of specific indication of melioidosis agent].

The reference-center of monitoring of agents of glanders and melioidosis carried out testing of reagents kits for diagnostic of agent of melioidosis and other close-related species of Burkholderiae in vitro. At the stage of specific identification of pathogenic Burkholderiae the diagnostic possibilities of commercial and experimental kits of reagents for express- and rapid analysis were evaluated. The criteria of evaluation of diagnostic value of kits of reagents were sensitivity, specificity and time of implementation of studies. The analysis with application of mono- and multi-locus amplification systems, including real-time polymerase chain reaction permitted during 5-6 hours to implement identification and differentiation of Burkholderia pseufomallei, B. thailandensis and B. cepacia.

Capillary and surface effects in the formation of nanosharp tungsten tips by electropolishing.

The DC electropolishing process has been applied to the sharpening of tungsten wires in 2 M KOH aqueous solution. Necking of tungsten anodes takes place in the vicinity of the electrolyte-air interface. This results in the creation of two separate wire parts with nanosharp tips. Using image analysis, we demonstrate that the products of electrochemical reactions on the wire surface form a film with distinguishable properties. Experimental estimates of the film density and interfacial tension show that the film is approximately 32 kg/m3 denser than the surrounding electrolyte and that its interfacial tension is approximately sigma approximately 0.2 mN/m. Using these estimates, we show that the film flow is predominantly driven by capillary forces. We hypothesize that the wire necking is caused by a bidirectional film flow originated from Plateau-Rayleigh instability and inherent to cylindrical films and jets.

[The outlook for improving the immunoglobulin preparations for the detection and identification of the causative agents of glanders and melioidosis]. / Perspektivy sovershenstvovaniia immunoglobulinovykh preparatov dlia obnaruzheniia i identifikatsii vozbuditelei sapa i melioidoza.

The paper summarizes the data concerning the production and study of monoclonal antibodies (MAb) to the diagnostically significant glanders and melioidosis bacillus antigens. It evaluates the efficiency of using MAb in the gel immunodiffusion and agglutination tests as a basis of new-generation preparations for fluorescent antibody assay, indirect hemagglutination test which are used while detecting and identifying pathogenic pseudomonads. The paper defines the quality indices for monoclonal luminescent immunoglobulins and provides evidence for the benefits of monoclonal diagnostic agents over polyclonal analogues.