ABSTRACT
The aim of this study is to estimiate the associations between the ANXA9, FABP3, and FABP4 genotypes and the breeding value for milk production traits (yields of milk, protein, and fat, as well as protein and fat percentage) in 975 Polish Holstein-Friesian cows. The frequencies of genotypes and alleles were determined. Statistically significant relations between the ANXA9 SNP and the breeding value for fat content, as well as the FABP4 SNP and the breeding value for protein content were found. The results indicated that selection for the ANXA9 GG and FABP4 GG animals might contribute to an decreased fat content and increased protein content in milk, respectively, in Polish Holstein-Friesian cows. Although verification in the further studies is needed.
Subject(s)
Annexins/genetics , Breeding , Fatty Acid-Binding Proteins/genetics , Milk/metabolism , Polymorphism, Single Nucleotide , Animals , Cattle , Fats/metabolism , Female , Gene Frequency , Milk/chemistry , Milk Proteins/genetics , Poland , Quantitative Trait, HeritableABSTRACT
The aim of this study was estimate the relations between CRH C22G and milk production traits (milk yield, yield of protein and fat, content of protein and fat in milk). The study was carried on 169 Polish Holstein-Friesian strain Red-and-White cows kept in the south-western region of Poland. The CRH C22G (exon 2) polymorphisms were detected using PCR-RFLP method. The genotype and allele frequencies were estimated and they were as follows: CC - 0.89, CG - 0.09, GG - 0.02; C - 0.93, G - 0.07. Statistically significant (P < or = 0.05) associations between CRH C22G genotypes and milk production traits like milk yield and protein yield in the second lactation were found. Moreover, the obtained results should be verified by conducting research on a larger group of animals and various cattle breeds.
Subject(s)
Corticotropin-Releasing Hormone/genetics , Milk/chemistry , Polymorphism, Single Nucleotide , Animals , Cattle , Dairying , Gene Frequency , Genetic Association Studies , Genotype , PolandABSTRACT
The aim of the conducted study was to evaluate correlation between genotypes and PRKAG3 compound genotypes and milk production traits (yield of milk, milk fat and milk protein, and protein and fat content in milk). The study covered a herd of 180 Jersey cows. PCR-RFLP method was used for genotyping. The frequencies of alleles that occur mostly and combined genotypes were as follows: T1526G G - 0.57, G 1609A G - 0.92 and for T1526G/G1609A TG/GG - 0.54. The results obtained in the study demonstrated the correlation between analyzed genotypes and selected milk production traits; however they are not statistically significant.
Subject(s)
AMP-Activated Protein Kinases/genetics , Cattle/genetics , Lactation/genetics , Polymorphism, Restriction Fragment Length , Quantitative Trait, Heritable , AMP-Activated Protein Kinases/metabolism , Alleles , Animals , Cattle/metabolism , Female , Gene Frequency/genetics , Genotype , Male , Milk/metabolism , Milk Proteins/genetics , Milk Proteins/metabolismABSTRACT
The study on polymorphism within the STAT5A gene (transition C6853T) was conducted using the PCR-RFLP method and AvaI restrictase. The study covered a herd of 723 cows of the Polish Red-and-White variety of Holstein Friesian breed, kept for dairy purposes in the Opole region, Poland. Two alleles (C and 7) of the analyzed STAT5A polymorphism were found in the studied herd. The alleles determined the occurrence of two genotypes: CC and CT. The homozygous TT genotype was not found. The STAT5A/AvaI allele frequencies were as follows: C--88.31% and T--11.69%, whereas the genotype frequencies were 76.6% for CC and 23.4% for CT. The analysis of associations between the STAT5A/AvaI polymorphism and milk utility traits considered in the study showed that these traits were different in animals with different STAT5A/AvaI genotypes.
Subject(s)
Milk/metabolism , Polymorphism, Genetic , STAT5 Transcription Factor/genetics , Alleles , Animals , Cattle , Deoxyribonucleases, Type II Site-Specific/chemistry , Female , Lactation , Poland , Polymerase Chain Reaction , Polymorphism, Restriction Fragment LengthABSTRACT
A total of 129 Limousin calves were used to investigate how leptin gene polymorphisms affect growth traits, such as body weight, average daily gain, wither height, sacrum height and chest girth in beef cattle. Two single nucleotide polymorphisms (SNPs) were genotyped, including the Sau3AI polymorphism in intron 2 and the A59V polymorphism in exon 3. The genotype and allele frequencies for each SNP and the haplotype frequencies for both SNPs were estimated in the studied herd. Statistical analysis revealed that the A59V polymorphism significantly affected the body weight at 210 days of age (P < or = 0.01) and the average daily gain between 3 and 210 days of age (P < or = 0.05) with Tas a desirable allele. No associations were observed between the Sau3AI polymorphism and the growth traits mentioned above. However, the average daily gain between 3 and 210 days of age was significantly higher in the CT/CT haplotype animals compared with the CC/CC and CC/CT individuals. These results indicated that selection for the A59V TT animals might contribute to an improved body weight in Limousin cattle.
Subject(s)
Cattle/growth & development , Cattle/genetics , Leptin/genetics , Polymorphism, Single Nucleotide , Animals , Body Weight/genetics , Body Weight/physiologyABSTRACT
The influence of levamisole on the course of myocarditis was investigated. It was found that levamisole decreased the intensity of subjective symptoms of disease as well as normalised electrocardiographical and immunological changes in greater part of patients.
