ABSTRACT
The influence of light water with the reduced content of heavy stable isotopes of oxygen and hydrogen (deuterium) on the cytogenetic status of irradiated animals was investigated. In mice, hybrids of the first generation (CBA x C57B1) F1, the increase in the output (two-fold at the dose of 2 Gy) of aberrant mitoses in the cells of bone marrow and the decrease in the duration of the mitotic index of the first cellular cycle occurred under the influence of the two week maintenance before the irradiation on light water with ppm 35 obtained by the method of rectification as compared with the irradiated animals that were kept on the distilled water. It has been discovered that 24 h after irradiation the number of leukocytes in the group which consumed light water is lower than that in the animals that were contained on the distilled water. Moreover, the cellularity of the bone marrow in the group which consumed light water was higher than that in the animals that were contained on distilled water. The prolonged application of light water before irradiation (for 14 days) led to an increase in the sensitivity of the chromosomal apparatus of mice to γ-irradiation against the background of an increase in the mitotic activity of cells.
Subject(s)
Cell Cycle/drug effects , Chromosome Aberrations/drug effects , Mitosis/drug effects , Water/administration & dosage , Animals , Bone Marrow Cells/drug effects , Bone Marrow Cells/radiation effects , Cell Cycle/radiation effects , Chromosome Aberrations/radiation effects , Deuterium/administration & dosage , Gamma Rays , Hydrogen/administration & dosage , Hydrogen/chemistry , Leukocytes/drug effects , Leukocytes/radiation effects , Mice , Oxygen Isotopes/administration & dosage , Whole-Body IrradiationABSTRACT
Eighty outbred CD-I male and female mice (initial body mass of 31-34 g) and 40 male Wistar rats (initial body mass of 280-360 g) were randomly distributed into the experimental and control groups by sex and body mass. According to the results of experiments, consumption of rectified light-isotope water over 30 days immediately after irradiation by gamma-quanta 60Co at 5 Gy and 6.5 Gy can increase survivability rate significantly, accelerate hemopoiesis recovery and prevent body mass loss in irradiated animals. Therefore, light-isotope water can be considered as an effective agent for treatment of the bone marrow syndrome.
Subject(s)
Isotopes/pharmacology , Radiation Injuries, Experimental/radiotherapy , Water/pharmacology , Acute Disease , Animals , Dose-Response Relationship, Radiation , Female , Hematopoiesis/drug effects , Hematopoiesis/radiation effects , Male , Mice , Rats , Rats, WistarABSTRACT
Effect of potable water with low content of heavy stable isotopes of oxygen and hydrogen on body mass and hematopoiesis was studied in intact laboratory animals. Outbred CD-I and first generation hybrid (CBA*C57B)F1 mice exhibited a statistical acceleration of body mass gain as a result of drinking rectified light-isotopic water with ppm 35; stimulating effect was noticed with respect to peripheral blood parameters and condition of the hematopoietic organs. The parameters under study did not go beyond boundaries of the physiological norm.
Subject(s)
Drinking Water/administration & dosage , Animals , Blood Platelets/drug effects , Body Mass Index , Bone Marrow Cells/drug effects , Cell Count , Crosses, Genetic , Erythrocytes/drug effects , Hematocrit , Hemoglobins/analysis , Hydrogen/metabolism , Isotopes/metabolism , Leukocytes/drug effects , Mice , Mice, Inbred C57BL , Mice, Inbred CBA , Oxygen Isotopes/metabolism , Weight Gain/drug effectsABSTRACT
Experiments were performed with outbreed mice CD-I and hybrid mice (CBA' C57BI)F1. Seven to 21 days before irradiation, the mice had continuously consumed light-isotope water (35 ppm) produced by rectification w/o secondary treatment. Dose of single gamma-irradiation ranged from 4 to 8.5 Gy. The radiomodifying effects included statistically reliable reductions in survivability and life span, loss of body mass, and decreases in marrow myelokaryocytes and peripheral leukocytes. In 8-9 days after irradiation, spleen mass in the experimental mice was found enlarged as compared with the controls.
