ABSTRACT
Interactions between different types of immune cells and organically-modified silica nanoparticles were studied. The silica particles functionalized with amine groups were prepared by sol-gel technique. Sheep immunoglobulin labeled with fluoresceine isothiocyanate was immobilized by adsorption onto the nanoparticles. The presence of the functional groups was confirmed by infrared absorption measurements. The level of immunocompetent cells interacting with the silica nanoparticles was estimated as the amount of fluorescence-bright cells by flow cytometry method. A low level of interaction of the peripheral blood lymphocytes with the silica nanoparticles was found. On the contrary, the macrophages are actively involved in interaction with the silica nanoparticles. The influence of different size of the silica nanoparticles and incubation time on viability and functional activity of peripheral blood lymphocytes and peritoneal macrophages were investigated.
Subject(s)
Lymphocytes/metabolism , Macrophages, Peritoneal/metabolism , Nanoparticles/chemistry , Silicon Dioxide/chemistry , Adsorption , Animals , Cells, Cultured , Female , Flow Cytometry/methods , Fluorescein-5-isothiocyanate/chemistry , Fluorescent Dyes/chemistry , Humans , Immunoglobulin G/chemistry , Immunoglobulin G/immunology , Lymphocytes/cytology , Macrophages, Peritoneal/cytology , Materials Testing , Molecular Structure , Particle Size , Sheep , Surface PropertiesABSTRACT
Interaction between aspartic acid and D-glucose, D-galactose, and D-fructose has been studied by isothermal titration calorimetry, calorimetry of dissolution, and densimetry. It has been found that D-glucose and D-fructose form thermodynamically stable associates with aspartic acid, in contrast to D-galactose. The selectivity in the interaction of aspartic acid with monosaccharides is affected by their stereochemical structures.
