ABSTRACT
Aim: The aim of the current research was to evaluate the antimicrobial effectiveness of chitosan-based tetracycline gel on periodontal pathogenic microorganisms. Materials and Methods: Preparation of chitosan-based tetracycline gel of 0.7% and 1% was done. In addition, 0.2 g of chitosan, 1% citric acid, and then 0.35 g of tetracycline in that order was added. Furthermore, 0.5 g of the tetracycline, which had been subjected to crushing and then stirring in a glass beaker to obtain a homogeneous gel was subjected to insertion into the syringe sterile. Such material prepared at the instance will attempt to investigate the sensitivity of bacteria in Surabaya to preserve the steadiness of the material. Evaluation of antimicrobial outcomes of chitosan-based tetracycline gel was performed against Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis. Results: The highest region of inhibition was noted at 28.26 ± 1.08 mm, 23.98 ± 2.02 mm against A. actinomycetemcomitans and P. gingivalis at 1% chitosan-based tetracycline gel, in pursuit by the 0.7% chitosan-based tetracycline gel at 15.12 ± 0.16 mm and 14.48 ± 1.22 mm region of inhibition, followed by a control group that exhibited 4.16 ± 1.29 mm and 4.82 ± 1.20 mm regions of inhibition against either of the pathogens. Conclusion: To conclude, the chitosan-based tetracycline gel is efficient in restraining the growth of A. actinomycetemcomitans and P. gingivalis bacteria. Tetracycline 1% gel-based chitosan exhibited maximum antibacterial action since it depicted the maximum inhibition region diameter versus tetracycline gel 0.7%.
ABSTRACT
Aim: The aim of this research was to identify the antimicrobial effectiveness of three different mouthwashes on periodontal pathogenic microorganisms. Materials and Methods: 2 periodontal disease-causing microorganisms, i.e., Aggregatibacter actinomycetemcomitans, and Porphyromonas gingivalis, were chosen for this investigation. Prior to commencing this research, a variety of branded and commercially obtainable mouthwashes were procured. Three oral rinses, namely HiOra, Hexidine, and Amflor, were chosen for the current research. The subculture of A. actinomycetemcomitans as well as P. gingivalis was performed by subjecting them to incubation for 48 to 72 hours at 35-37°C. The disk diffusion method was employed to evaluate the antibacterial efficiency of the extract in opposition to the pathogens tested. The zone of inhibition was calculated in millimeters. The mean value of every sample was documented. Results: Hexidine oral rinse in pursuit by Amflor as well as HiOra oral rinse exhibited the highest zone of inhibition in opposition to A. Actinomycetemcomitans and P. gingivalis. The differences amid the groups were statistically significant with a P value < 0.001. Conclusion: The current research concluded that amid the three different oral rinses employed in the current research, Hexidine oral rinse exhibited greatest antimicrobial effectiveness versus Amflor and HiOra mouthrinse.
