[Changes of parameters of the functional state of the organism and oxidative stress in patients after surgical revascularization at early stage of restorative treatment]. / Izmeneniia pokazatelei funktsional'nogo sostoianiia organizma i oksidativnogo stressa u bol'nykh posle khirurgicheskoi revaskuliarizatsii miokarda na rannei stadii vosstanovitel'nogo lecheniia.

Early complex restorative treatment with individualized aerobic physical exercise in 2-4 weeks after surgical myocardial revascularization significantly increased physical working capacity and cardiorespiratory reserve of the body. Pronounced oxidative stress which had been present after myocardial revascularization decreased under the action of restorative therapy. Most pronounced increases of total physical capacity and cardiorespiratory reserve and lowering of oxidative stress occurred in patients with lowest initial parameters of the functional state of the organism.

[Features of temperature dependence of the Na+,K+-ATPase reaction in normal and tumorous brain tissue]. / Osobennosti temperaturnoi zavisimosti Na+,K+-ATP-aznoi reaktsii v normal'noi i opukholevoi tkani mozga.

It has been shown that in the enzyme preparations (EP) from normal brain tissue (NBT) a typical break on the Arrhenius plot appeared at 20-22 degrees C, nH for Na+ and K+ exceeding 1.7 and 1.4, respectively. In EP from tumoural brain tissue (TBT) no break on the Arrhenius plot at 20-22 degrees C was revealed, but it appeared at 27.5 + 30.5 degrees C. The nH for Na+ with Na+,K+-ATPase from TBT was only 0.9, but the cooperative binding of K+ was preserved (nH = 1.3). Electrophoregrams (EP) from TBT showed additional protein bands. The urea and digitonin treatment of EP from NBT induced a break on the Arrhenius plot at 27.5-30.5 degrees C. It is suggested that the break at 27.5-30.5 degrees C is, probably, accompanied by local changes in the conformation of protein components of the enzyme.

[Characteristics of cooperative binding of Na+ and K+ with Na+,K+-ATPase depending upon its oligomeric structure]. / Osobennosti kooperativnogo sviazyvaniia Na+ i K+ s Na+,K+-ATPazoi mozga v zavisimosti ot ee oligomernoi struktury.

It has been shown that the desensibilization of the enzymic preparations of Na+, K+-ATPase by urea, DS-Na, digitonin and CHAPS reduces differently the amount of alpha beta-protomer in the enzymic preparations and the Hill coefficients of Na+ and K+. The factors (urea, DS-Na) which cause a more pronounced decrease in the amount of beta-protomer reduce the nH of Na+ for Na+, K+-ATPase and nH of K+ for Na+, K+-ATPase and K+-pNPPase to unit. The analysis of the effects of ATP and pNPP indicates that ATP has a protective effect only in the case of urea and DS-Na, but this effect is not exerted by pNPP (nonallosteric substrate). A conclusion is drawn that cooperative interactions of Na+, K+-ATPase from the brain with Na+ require more higher level of the oligomeric structure of enzyme than cooperative interactions with K+. At the same time these cooperative interactions in the both cases need subunits interactions in the protomer and interactions between cation sites with relatively high affinity.