ABSTRACT
Human papillomavirus (HPV) infection is common among sexually active individuals and in some cases this infection could coincide with pregnancy in women. In this study, we present our results from investigation for HPV presence in the samples of 50 women with spontaneous abortions. Detection and typing of HPV were carried out by polymerase chain reaction (PCR), using the primers designed to amplify E6/E7 gene sequences of HPV-16, 18 and L1 gene region of HPV-6, 11. HPV DNA was found in 1.5% (3/50) of the clinical samples tested (HPV-16 in one patient, HPV-18--in another one and HPV-16 and HPV-18--in the third patient. Our results support the hypothesis that HPV might be associated with some cases of spontaneous abortions. However, a bigger number of cases are needed for further studies to assess the actual risk of this virus in pregnancy.
Subject(s)
Abortion, Spontaneous/virology , Human papillomavirus 16/isolation & purification , Human papillomavirus 18/isolation & purification , Papillomavirus Infections/complications , Abortion, Spontaneous/etiology , Female , Genes, Viral , Humans , Papillomavirus Infections/virology , Placenta/virology , Reverse Transcriptase Polymerase Chain ReactionABSTRACT
The review presents the main molecular virology techniques for detection, identification and analysis of human papillomaviruses (HPVs). Theoretical and practical bases of hybridization techniques (Southern blot, Dot blot, In situ hybridization, Filter in situ hybridization FISH, Hybrid Capture Assay, HCA), amplification techniques (polymerase chain reaction, PCR and variants--multiplex PCR, reverse transcription PCR, real-time PCR) and sequencing techniques(sequencing analysis, single-strand conformation polymorphism--SSCP) are discussed.
