A double-blind controlled clinical trial to evaluate the effects of nasal therapy with Vrihatajivakadya oil on different viscosities in patients with migraine.

BACKGROUND: Migraine, characterized by pain of a specific type in one half of the head has a close resemblance with Ardhavabhedaka described in Ayurveda. Nasya karma (nasal therapy) with Vrihatajivakadya oil is indicated in Ardhavabhedaka. Low viscosity oil (LVO) and medium viscosity oil (MVO) prepared by Snehapaka (a specific Ayurveda method for preparation of oil) are advocated in different classical Ayurveda texts for N.karma. THE OBJECTIVES: This study was done to assess the effects of Vrihatajivakadhya oil on different viscosities in N.karma for the better Ayurveda management of migraine. MATERIAL AND METHODS: In this double-blind randomized controlled trial a total of 90 patients were randomly divided into two groups for N.karma with oil of different viscosities. In the group treated with LVO, 44 patients completed their treatment and one patient was lost in follow-up. In another group treated with MVO, 45 patients were enrolled and completed the intervention. N.karma was done with this oil in the dose of 6 drops per nostril for the duration of 14 days for each participant. The follow-up was done on the 15th day and 45th days. The assessment was done by the Migraine Disability Assessment Score (MIDAS) and Migraine Specific Quality of Life Assessment scale. RESULT: During the trial, significant improvement in both the groups on both parameters was observed. No adverse event was noticed during the study. CONCLUSION: N.karma with MVO had better improvement. No adverse event was noticed during the study. N.karma with these oils is effective and safe for migraine.

Reaction of 7α-bromo-6-nitrocholest-5-enes with hydrazine: Formation of steroidal pyrazolines and molecular docking against SARS-CoV-2 omicron protease.

The present work reports simple and effective protocol for preparing 6α-nitro-5α-cholestano[7α,5-cd] pyrazolines (4-7) by the reaction of 7α-bromo-6-nitrocholest-5-enes (1-3) with hydrazine hydrate under reflux [the substrate (2) gave products (5) and (6) and the later on acetylation with AC2O/Py gave (7)]. In the case of reaction of 3ß-hydroxy analogue (3) with hydrazine, however, 6α-nitro-5α-cholestano [3α,5-cd] pyrazoline (8) and 6α-nitro-3ß, 5-oxido-5ß-cholestane (9) were obtained. The probable mechanism of the formation of pyrazolines has also been outlined. In the current pandemic coronavirus disease 2019 scenario, the in-silico study was performed with reactants (1-3), their products (4-9) against SARS-CoV-2 omicron protease (PDB ID:7T9L) for knowing significant interactions between them. Docking results give information that both reactants and products have binding energies ranges from -5.7 to 7.7 kcal/mol and strong interactions with various hydrophilic and hydrophobic amino acids such as ASP, PRO, PHE, SER and LEU which are significant residues playing important role in SARS-CoV-2 Omicron main protease (Mpro).

Eliminating Data Duplication in CQA Platforms Using Deep Neural Model.

Primary research to detect duplicate question pairs within community-based question answering systems is based on datasets made of English questions only. This research put forward a solution to the problem of duplicate question detection by matching semantically identical questions in transliterated bilingual data. Deep learning has been implemented to analyze informal languages like Hinglish which is a bilingual mix of Hindi and English on Community Question Answering (CQA) platforms to identify duplicacy in questions. The proposed model works in two sequential modules. First module is a language transliteration module which converts input questions into a mono-language text. The next module takes the transliterated text where a hybrid deep learning model which is implemented using multiple layers is used to detect duplicate questions in the mono-lingual data. The similarity between the question pairs is done utilizing this hybrid model combining a Siamese neural network with identical capsule network as the subnetworks and a decision tree classifier. Manhattan distance function is used with the Siamese network for computing the similarity between questions. The proposed model has been validated on 150 pairs of questions which were scrapped from various social media platforms, such as Tripadvisor and Quora which achieves accuracy of 87.0885% and AUC-ROC value of 0.86.

Compare the Efficacy of Chlosite Gel as an Adjunctive Therapy after Scaling and Root Planing.

Aim: The present study was carried out to evaluate the clinical efficacy of scaling and root planing (SRP) alone and commercially available chlosite gel adjunctive to SRP in the treatment of chronic periodontitis. Materials and Methods: A total of 416 sites selected from 26 patients presenting with chronic periodontitis (age group ≥30 years) of both sexes, with pocket depth of ≥6 mm were recruited for the study. A split-mouth design was employed with one site receiving SRP alone (Group I) and the other receiving SRP followed by placement of commercially available chlosite gel (Group II) and all the clinical parameters-Plaque index (PI), Gingival index (GI), Probing Pocket depth and Clinical attachment loss (CAL) were recorded at baseline, 1 month, 3 months, and 6 months. Results: Results demonstrated that significant reduction in PI and GI scores were observed in both groups till the end of the study period (6 months). Probing depth and CAL also showed significant improvement in both the groups. However, Group II (sites which received SRP + Chlosite gel) showed greater improvements in all of these parameters compared to Group I (sites which received SRP only). Conclusion: The findings of this study suggest that the locally delivered commercially available chlosite gel along with mechanical debridement, resulted in a clinically meaningful improvement in all the clinical parameters.

Assessment of free radicals and reactive oxygen species milieu in nanoparticles adjuvanted outer membrane proteins vaccine against Salmonella typhi.

In this study, calcium phosphate nanoparticles-based (STCNV) and montanide oil adjuvant vaccine (STOAV) containing outer membrane proteins (Omps) of S. Typhi were evaluated for inducing oxidative stress indicators [reduced glutathione (GSH), lipid peroxidation (LPO), catalase, superoxide dismutase (SOD), and total protein] in the tissues of mice after vaccination. The GSH levels though slightly high in the liver, kidney, and lungs of STCNV group were not significantly different from STOAV and the control group (STC). There was no significant difference in LPO levels in any group for any tissue. The significantly lower activities of catalase were observed in the kidney and lungs of the STCNV group as compared to STOAV and STC group, while in the liver, STCNV group revealed lower catalase activity in comparison to the control group. No significant difference in the SOD activities between the two vaccinated groups was observed. The total protein contents in all the organs showed no significant difference in the vaccinated and the control group. The vaccines may induce long-term inflammatory response and consequently damage vital organs; this study revealed no long-term oxidative stress in all the three vital organs, suggesting that these vaccines may not cause oxidative damages in the vital organs of mice.

Calcium phosphate adjuvanted nanoparticles of outer membrane proteins of Salmonella Typhi as a candidate for vaccine development against Typhoid fever.

Introduction. The conventional adjuvants used in vaccines have limitations like induction of an imbalanced Th1 and Th2 immune response. To overcome this limitation, novel adjuvants and newer forms of existing adjuvants like calcium phosphate nanoparticles are being tested.Hypothesis/Gap Statement. Calcium phosphate adjuvanted outer membrane proteins vaccine may work as an efficient, safe and cost effective vaccine against Salmonella Typhi.Aim. Our goals were to evaluate the potential of calcium phosphate nanoparticles as an adjuvant using outer membrane proteins (Omps) of Salmonella Typhi as antigens for immune response, with montanide (commercially available adjuvant) as control, and its toxicity in rats.Methodology. Calcium phosphate adjuvanted outer membrane proteins nanoparticles were synthesized and characterized. The efficacy of vaccine formulation in mice and toxicity assay were carried out in rats.Results. The calcium phosphate nanoparticles varying in size between 20-50 nm had entrapment efficiency of 41.5% and loading capacity of 54%. The calcium phosphate nanoparticle-Omps vaccine formulation (nanoparticle-Omps) induced a strong humoral immune response, which was significantly higher than the control group for the entire period of study. In the montanide-Omps group the initial very high immune response declined steeply and then remained steady. The immune response induced by nanoparticle-Omps did not change appreciably. The cell mediated immune response as measured by lymphocyte proliferation assay and delayed type hypersensitivity test showed a higher response (P<0.01) for the nanoparticles-Omps group as compared to montanide-Omps group. The bacterial clearance assay also showed higher clearance in the nanoparticles-Omps group as compared to montanide-Omps group (approx 1.4%). The toxicity analysis in rats showed no difference in the values of toxicity biomarkers and blood chemistry parameters, revealing vaccine formulation was non-toxic in rats.Conclusion. Calcium phosphate nanoparticles as adjuvant in vaccines is safe, have good encapsulation and loading capacity and induce a strong cell mediated, humoral and protective immune response.

Comparative study of biogenically synthesized silver and gold nanoparticles of Acacia auriculiformis leaves and their efficacy against Alzheimer's and Parkinson's disease.

The present article reports the biogenic synthesis of silver nanoparticles (AgNPs) and gold nanoparticles (AuNPs) from the extract of Acacia auriculiformis (AA) leaves using biogenic approach. Several spectral and morphological studies namely UV-vis, Fourier transform infrared (FT-IR), tunneling electron microscopy along with selected area electron diffraction (TEM/SAED), scanning electron microscopy along with energy dispersive X-ray (SEM-EDX) and X-ray diffraction (XRD) were carried out which ascertains the successful formation of silver nanoparticles (AgNPs) and gold nanoparticles (AuNPs) starting from Silver nitrate and Chloroauric acid respectively. On the basis of TEM/SAED and SEM-EDX, AgNPs were found to be more regular with smaller particle size and hence they were selected for biological studies. Thermal techniques like thermo gravimetric analysis (TGA) and differential thermal analysis (DTA) were also performed to study the comparative thermal stability of AgNPs and AuNPs where AgNPs were found to be thermally more stable. Several biophysical techniques including Thioflavin T assay, ANS assay, Rayleigh scattering method and turbidity assay were also performed. These assays confirm that AgNPs possess better inhibitory property. Moreover, antioxidant activity of AgNPs was also carried out using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and AgNPs were found to be good antioxidant.

Periosteum as a lateral pedicle graft for the treatment of single tooth root recession: A novel approach.

The treatment of gingival recession defects is indicated for esthetic and functional reasons to reduce root sensitivity, to remove muscle pull, to create or augment keratinized tissue, and to prevent disease progression. The presence of sufficient amount of periosteum adjacent to gingival recession defects makes it a suitable graft. The adult human periosteum is a highly vascular connective tissue with immense regenerative potential. It contains fibroblasts, osteogenic progenitor cells, and stem cells as a result of which it has the ability to differentiate into fibroblast, osteoblast, chondrocytes, adipocytes, and skeletal myocytes. The tissue provided by these cells includes cementum with periodontal ligament fibers and bone. Therefore, in the present case, periosteum has been used as a lateral pedicle graft for the coverage of the single tooth gingival recession (mandibular central incisor). The procedure is justified by the evidence that periosteum is capable of proliferation and osteogenesis after injury.

Comparative study of nonabsorbable and absorbable barrier membranes in periodontal osseous defects by guided tissue regeneration.

BACKGROUND: Periodontal invasion of furcation area in multirooted teeth represents one of the most demanding therapeutic challenges in periodontics. Furcation therapy includes various treatment modalities like either maintenance or elimination of furcation or increased access to furcation area. Recent treatment modalities include regenerative procedures like placement of different type of bone grafts with nonabsorbable or absorbable barrier membranes, through guided tissue regeneration. This study compared the clinical efficacy of nonabsorbable barrier membrane with absorbable membrane when used with hydroxyapatite bone graft (G-Graft) in grade II buccal furcation defects in mandibular 1st molars. MATERIALS AND METHODS: Fourteen subjects with bilateral grade II buccal furcation defects in lower 1st molars were selected and treated in a split-mouth design. After phase I therapy, molars were divided randomly into two groups for the treatment with either resorbable or nonresorbable membrane in conjunction with G-Graft in both groups. RESULTS: All the clinical parameters recorded showed statistically significant improvement in both the groups but no significant difference between two groups was observed. CONCLUSION: Both nonabsorbable and absorbable barrier membranes were equally effective in treating grade II buccal furcation defects in lower molars when used in conjunction with G-Graft except with respect to horizontal bone fill in which absorbable barrier membrane showed better results.

Harmonious functional and aesthetic correction of severe localised pregnancy-induced gingival enlargement associated with capillary haemangioma.

Gingival enlargement is a clinical condition that has been widely studied and is directly associated with specific local or systemic conditions. Pregnancy has been presented to increase susceptibility to gingival inflammation. Sex hormones are believed to be a risk factor for periodontitis because of their ability to proliferate specific periodontal microorganisms and affect host immunological response, but it is unclear whether pregnancy gingivitis exposes or proceeds to periodontitis. In this case report, the patient reported with severe localised enlarged gingival mass which initiated when she was pregnant. After parturition, gingival enlargement was persisting and causing functional and aesthetic problem. Enlargement did not resolve even after non-surgical therapy; therefore, surgical excision of the entire enlarged gingival mass was preformed. Histopathological examination revealed capillary haemangioma. No evidence of malignancy was seen. No recurrence was seen even after 2 years of follow-up.

Gastric antisecretory and antiulcer activities of Cedrus deodara (Roxb.) Loud. in Wistar rats.

ETHNOPHARMACOLOGICAL RELEVANCE: Cedrus deodara (Roxb.) Loud. is used in Ayurvedic medicine to treat peptic ulcer. AIM OF THE STUDY: To evaluate the gastric antisecretory and antiulcer activity of Cedrus deodara. MATERIALS AND METHODS: The volatile oil extracted by steam distillation of Cedrus deodara wood was examined for its gastric antisecretory and antiulcer effect in the pylorus-ligated rat model and ethanol induced gastric lesions in rats. RESULTS: The volatile oil showed significant antisecretory activity as evidenced by decreased gastric fluid volume, total acidity, free acidity and increase in the pH of the gastric fluid in pylorus-ligated rats. Our studies also revealed that pretreatment with Cedrus deodara significantly reduced the number of ulcer, ulcer score and ulcer index in pylorus-ligated and ethanol treated rats. The antiulcer activity of Cedrus deodara is further supported by histopathological study which showed protection of mucosal layer from ulceration and inflammation. CONCLUSION: The present findings conclude that volatile oil of Cedrus deodara wood has potent antisecretory and antiulcer effects and justify the traditional usage of this herb to treat peptic ulcers.