ABSTRACT
L-Cysteine (L-cys) plays very crucial role in biological systems. The study reports the colorimetric detection of L-cys at nanomolar level using chitosan capped Ag decorated NiS nanocomposite (chit-Ag/NiS NCs).The chemical reduction and co-precipitation methods were adopted to prepare chit-Ag/NiS NCs. The fabricated NCs was characterized by X-ray diffraction (XRD), fourier-transform infrared spectroscopy (FT-IR), FT-Raman, scanning electron microscopy (SEM), thermogravimetric analysis (TGA), high-resolution transmission electron microscopy (HR-TEM), energy-dispersive X-ray spectroscopy (EDS) and X-ray photoelectron spectroscopy (XPS). The chit-Ag/NiS NCs particularly detect L-cys even in other amino acids presence. The chit-Ag/NiS NCs showed the surface charge of -26 ± 39.9 mV. The detection of L-cys was indicated by disappearance of yellowish-brown color of Chit-Ag/NiS NCs to colorless. A good linear correlation was found between absorbance vs logarithmic concentration of L-cys (1 µM to 1 nM) with R2 value of 0.99. The chit-Ag/NiS NCs impregnated cotton swabs was prepared for real time detection of L-cys and the prepared probe was found to be highly selective and specific. The effect of pH, temperature and salinity influencing the L-cys detection was studied. Also, the antimicrobial activity of Chit-Ag/NiS NCs was investigated against gram negative (E. coli) and gram positive (B. subtilis) bacteria.
