Electrified liquid - liquid interface strategy for sensing lactic acid in buttermilk extract.

Lactic acid (LA) serves as a freshness marker in certain foods. In the present work, electrified interfaces of different nature (i.e., liquid-liquid and liquid-organogel) have been developed for the quantification of LA. Electrochemical sensing of LA at the liquid-organogel interface revealed that adsorptive stripping voltammetry, with a preconcentration time of 500 s offered better sensitivity. Electroanalytical ability of LA under optimized conditions displayed a detection limit of 0.97 µM and 0.71 µM with sensitivity of 2.84 nA µM-1 and 3.59 nA µM-1 for liquid-liquid and liquid-organogel interfaces respectively. Quantification of LA using the developed methodology has been demonstrated in buttermilk as the real matrix. Analysis demonstrate that electrified liquid-liquid and liquid-organogel interfaces are promising approach for sensing LAin buttermilk extract.

Data on host specificity and symbiotic association between indigenous Rhizobium BD1 strain and Vigna radiata (green gram).

The main objective of this study is to use bio-inoculants in relative to specific legume plant diversity for, enhanced nodulation and plant growth. Method involves organically based selection of 36 rhizobial strains, of which 6 strains were isolated to assess the efficiency of relative host-specific inoculation on nodulation and development in legumes viz. Vigna radiata. All promising combinations of the preferred rhizobial strain inoculants were tested under sterile conditions for improving nodulation and to screen the best isolate to be evaluated for its enhanced characteristics through inoculation by field trial in various soils. It was observed that the strains from Bhadrachalam forest BD1 are highly host specific for Vigna radiata plants and when inoculated, improved nodulation and enhanced plant growth. Because of the novel characters in BD1, further studies were carried out and was identified as Rhizobium sp. BD1 (NCBI Accession no. MT577595). The percentage of nitrogen content in Vigna radiata ranged between 1.2% to 2.9%. This Rhizobium sp. BD1 was tested for the unraveling and amelioration of crop production in barren, polluted and agricultural soils which showed enhanced characteristics in Vigna radiata plants. This method may be employed across the globe of same climatic conditions for the retrieval of plants in soils that carry agriculture unsuccessfully.