ABSTRACT
Cancer cell heterogeneity (CCH) is crucial in understanding cancer progression and metastasis. The CCH is one of the stumbling blocks in modern medicine's therapeutics and diagnostics . An in-vitro model of co-culture systems of MCF-7, HeLa, HEK-293, with THP-1 cells showed the occurrence of EpCAM positive (EpCAM+) and EpCAM negative (EpCAM-) heterogenetic cancer cell types labeled with the Quantum Dot antibody conjugates (QDAb). This in-vitro model study could provide insights into the role of rare cancer cells manifestation and their heterogeneity in metastatic progression and risk for severe infections in these patients. We successfully report the presence of CCH based on the fluorescence ratios of the co-cultured cancer cells when treated with the QDAb. These short-term mimic co-cultures give a compelling and quite associated model for assessing early treatment responses in various cancers.
Subject(s)
Immunoconjugates , Neoplasms , Quantum Dots , Coculture Techniques , Epithelial Cell Adhesion Molecule/metabolism , HEK293 Cells , HumansABSTRACT
Quantum dots (QD) with chemical composition QD CdSe / ZnS _ Ni 650 were successfully synthesized using a hydrothermal method and chemical precipitation. The nanocrystalline phase of the nanostructures was isolated and characterized using X-ray diffraction (XRD). The mean crystalline size doped core/shell Ni-dopant range was 9.0 ± 2.0 nm. The ferromagnetic data revealed the magnetic behaviour of QD CdSe / ZnS _ Ni 650 . The optical absorption measurements of these QDs were in the UV-visible light range 200-800 nm for a band gap value of 2.11 eV for QD CdSe / ZnS _ Ni 650 . This means that pure QD CdSe 650 and QD CdSe / ZnS _ Ni 650 underwent a redshift when compared with bulk CdSe. For QD CdSe / ZnS _ Ni 650 there was successful uptake by cell lines including HeLa and MCF-7 for bioimaging and sorting applications.
Subject(s)
Cadmium Compounds , Quantum Dots , Selenium Compounds , Cadmium Compounds/chemistry , Humans , Quantum Dots/chemistry , Selenium Compounds/chemistry , Sulfides/chemistry , Zinc Compounds/chemistryABSTRACT
Chitosan (CH) capped inorganic nanomaterials have been considered as significant antibacterial materials in the clinical field. This work shows the synthesis of two new different antibacterial composite films as a result of the incorporation of CH capped copper oxide (CHCuO) and copper (CHCu) nanoparticles (NPs). Here, CHCuO and CHCu NPs were achieved by a facile chemical reduction of Cu2+ ions using sodium hydroxide and ascorbic acid. TEM analysis revealed the morphology as rod-type nanoflakes for CHCuO and a spherical shape for CHCu NPs with ~7⯱â¯2â¯nm size. Antimicrobial activity of the developed materials was studied by the inhibition zone method, against both gram-negative and gram-positive bacteria. The antimicrobial activity revealed that the CHCuO NPs and CHCuO-CH film showed a higher inhibition zone than the other nanomaterials. The results suggested that the synthesized materials can be used in wound dressing applications.
Subject(s)
Chitosan/chemistry , Copper/chemistry , Copper/pharmacology , Drug Resistance, Bacterial/drug effects , Metal Nanoparticles/chemistry , Nanocomposites/chemistry , Anti-Bacterial Agents/chemistry , Anti-Bacterial Agents/pharmacology , Bacillus/drug effects , Capsules , Escherichia coli/drug effects , Surface Properties , TemperatureABSTRACT
Efficiency of white-rot fungi-Stereum ostrea (S. ostrea) as a test culture and Phanerochaete chrysosporium (P. chrysosporium) as a reference culture in colour removal from a textile dye, Remazol brilliant blue-19 (RBB-19) in medium was compared in this study. S. ostrea was more efficient than P. chrysosporium in decoloration process. Different parameters pH, temperature, sources of carbon and nitrogen, stationary and shaking conditions were optimized for bleaching of dye by the fungal cultures. Optimal growth conditions for decoloration of dye by both cultures were pH 5.0, temperature 35 °C, glucose and fructose as best carbon source at 1 % level, peptone and urea as best nitrogen source and shaking conditions (150 rpm). Culture broth free of colour (99 % of decoloration) was achieved with S. ostrea as against 70 % decoloration by P. chrysosporium on 6th day of incubation. Adsorption of dye to fungal biomass as reflected by colour coating on biomass and participation of lignolytic enzymes in colour removal appeared to be mechanisms involved in decoloration process. The ability of both fungal cultures in removal of colour in effluents with dyes collected from silk saree-weaving cottage unit was tested. S. ostrea was also found to be more effective in colour removal from effluent. S. ostrea appears to be a promising culture for application of bioremediation in decoloration of dyes.
