Advancements in laundry wastewater treatment for reuse: a review.

Laundry related activities produce huge quantity of wastewater that is very rich in lint, dyes, surfactants, and detergents. The large amount of laundry wastewater (LWW) is generated by extensive human activities. LWW needs to be treated in order to tackle the challenging problem of water pollution and to accomplish water sustainability. To achieve this success, LWW can be reused for several purposes such as irrigation, construction activities, vehicle washing etc. However, there are several challenges in the reclamation of LWW like effective handling of the wastewater and meeting the regulatory criteria. Based on the literature review it seems that a single treatment process is not sufficient to treat LWW up to acceptable reuse standards. To that end, different treatment chains have been proposed: i) hybrid processes combining membrane filtration with adsorption; ii) advanced oxidation process using ultraviolet (UV) and hydrogen peroxide (H2O2); iii) coagulation-flocculation combined with sand filtration; iv) combination of ozonation process, adsorption, and ultrafiltration (UF). This review paper discusses the selection of suitable treatment technology depends on several factors: i) a well-designed equipment, ii) cost-effectiveness of treatment method and iii) desired characteristics of the treated water. The review paper also presents solutions for treatment and reclamation of LWW.

Identifying economical route for crude glycerol valorization: Biodiesel versus polyhydroxy-butyrate (PHB).

Crude glycerol, a by-product of biodiesel industry, has been used for production of biodiesel and polyhydroxy-alkanoates. But question is: which product is economically favorable using crude glycerol as substrate? In this study, energy balance and economic assessment has been carried out for crude glycerol valorization for B10 biodiesel and polyhydroxy-butyrate (PHB) production. For same quantity of crude glycerol utilized, energy ratio for B10 production was higher than PHB production while unit production cost for B10 was lower than that of PHB. For 50 million L plant capacity of biodiesel, unit production cost was 0.77 $/L B10 while for 2 million kg plant capacity of PHB, unit production cost was 4.88 $/kg PHB. Thus, in present scenario production of biodiesel seems economically better than production of PHA with crude glycerol as raw material. This study is useful for researchers, environmental scientists and industries in identifying effective route for crude glycerol valorization.

Techno-economic analysis for extracellular-polymeric substances (EPS) production using activated sludge fortified with crude glycerol as substrate and its application in leachate treatment.

Economic assessment of bio-flocculant production process has been carried out by SuperPro Designer where extracellular-polymeric substances (EPS) were produced using activated sludge fortified with crude glycerol in fermenter followed by centrifugation. Considering EPS concentration of 60 g/L in production fermenter at 96 h, the unit production cost for slime EPS was estimated to be $ 0.95/L. The unit price of S-EPS was sensitive to inoculum size and EPS productivity (EPS concentration and fermentation time) in the fermented broth. Economic analysis was also conducted for EPS aided leachate treatment. The unit leachate treatment cost was 7.78 $/m3 and was sensitive to S-EPS unit production cost. To get same leachate treatment cost as current industrial practice (4 $/m3), S-EPS unit production cost should lower down to $ 0.5/L. The process has several advantages: 1) sludge and crude glycerol valorization for bio-flocculant production 2) Leachate treatment using environment friendly bio-flocculant.

Biopesticide and formulation processes based on starch industrial wastewater fortified with soybean medium.

The aim of this study was to produce Bacillus thuringiensis-based biopesticide using starch-producing industry wastewater (SIW) fortified with soybean medium and optimize the formulated product using different adjuvants. This study was necessary as low endotoxin concentration is obtained in formulated biopesticide when SIW alone is used as fermentation medium. The fermentation runs were conducted using SIW alone and SIW fortified with 25% soybean (w/v) medium in 2000 L and 150 L bioreactor, respectively. SIW supplemented with soybean medium showed an increase in cell count (from 1.95 × 108 to 1.65 × 109 CFU mL-1), spore synthesis (from 1.5 × 108 to 1.35 × 109 CFU mL-1) and endotoxin concentration (from 436 to 1170 µg mL-1) when compared to SIW medium alone. The fermented broth was concentrated using continuous centrifugation and adjuvants were added for biopesticide formulation in order to enhance its resistance against UV rays and rainfastness. Entomotoxicity of the formulation produced using fermented broth of SIW fortified with soybean (38,000 IU µL-1) was higher than that obtained by SIW medium alone (21,000 IU µL-1), commercial biopesticide Foray 76B (20,000 IU µL-1) and Btk sander's (12,500 IU µL-1).

Cost, energy and GHG emission assessment for microbial biodiesel production through valorization of municipal sludge and crude glycerol.

In this study, cost simulations were made based on 20 million L blended biodiesel B-10 production per year using INRS and conventional process. In case of INRS process, microbial lipid was produced by T. oleaginosus using washed municipal secondary sludge fortified with crude glycerol while lipid was extracted from wet biomass using biodegradable surfactant and petroleum-diesel (PD). The conventional process uses commercial substrates for lipid production and organic solvents for lipid extraction from dry biomass. The unit B-10 production cost of INRS process was estimated to be $ 0.72/L for an annual capacity of 20 million L, which is 9.5 times more economical than conventional biodiesel production process. For INRS process, the unit B-10 biodiesel production cost was sensitive to plant capacity and lipid productivity during the fermentation. INRS process exhibited positive net energy gain and positive GHG capture, which proves to be energetically and environmentally viable.

Bioconversion of waste (water)/residues to bioplastics- A circular bioeconomy approach.

Research insight into the technical challenges of bioplastics production has revealed their confoundedness in their niche markets and struggles to enter the mainstream. There is an increasing problem of waste disposal and high cost of pure substrates in polyhydroxyalkanoates (PHA) production. This has led to the future need of upgrading the waste streams from different industries into the role of feedstocks for production of PHA. The review covers the latest developments in using wastes and surplus materials for PHA production. In addition to inexpensive carbon sources, efficient upstream and downstream processes and recycling of waste streams within the process are required to maintain the circularity in the entire process. A view on the link between circular bioeconomy and PHA production process covering the techno-economic, life cycle assessment and environmental aspects has also been provided. Furthermore, the future perspectives related to the topic have also been discussed.

Production of Bacillus thuringiensis based biopesticide formulation using starch industry wastewater (SIW) as substrate: A techno-economic evaluation.

In this study, cost simulation was made to produce Bacillus thuriengiensis based biopesticide formulation using starch industry wastewater (SIW) as substrate. The results obtained at pilot plant (2000L capacity fermenter) were used for cost simulation of the process. The unit production cost for annual production of 5 million L of formulated biopesticide (20.2 Billion International Units (BIU)/L) was estimated to be $ 2.54/L, which is competitive to chemical pesticides. The techno-economic evaluation revealed that the profitability of the biopesticide manufacturing process was sensitive to the plant capacity and selling price of the biopesticide. The manufacturer should target 5 million L annual plant capacity and selling price of $ 15/L for payback period to be less than 5 years. The process serves many advantages (1) alternate disposal or bio-valorisation of industry wastewater and (2) use of industry wastewater as inexpensive carbon source reducing cost of raw materials for fermentation.

A review on variation in crude glycerol composition, bio-valorization of crude and purified glycerol as carbon source for lipid production.

Crude glycerol (CG) is a by-product formed during the trans-esterification reaction for biodiesel production. Although crude glycerol is considered a waste stream of the biodiesel industry, it can replace expensive carbon substrates required for lipid production by oleaginous micro-organisms. However, crude glycerol has several impurities, such as methanol, soap, triglycerides, fatty acids, salts and metals, which are created during the trans-esterification process and may affect the cellular metabolism involved in lipid synthesis. This review aims to critically present a variation in crude glycerol composition depending on trans-esterification process and impact of impurities present in the crude glycerol on the cell growth and lipid accumulation by oleaginous microbes. This study also draws comparison between purified and crude glycerol for lipid production. Several techniques for crude glycerol purification (chemical treatment, thermal treatment, membrane technology, ion-exchange chromatography and adsorption) have been presented and discussed with reference to cost and environmental effects.

Energy balance for biodiesel production processes using microbial oil and scum.

Biodiesel production using microbial oil is a promising technology. The main aim of this study is to check practical feasibility (in terms of energy balance) of different biodiesel production processes. Mass and energy balance of biodiesel production have been performed for 3 separate processes: (1) microbial lipid production from T. oleaginosus using waste substrates followed by INRS downstream process (2) microbial lipid production from pure substrate using R. toruloides followed by traditional and INRS downstream process and 3) oil extraction from scum and conversion to biodiesel. It was found that employing waste substrates like crude glycerol and municipal sludge in fermentation reduced the energy input by 50%. While employing biodegradable surfactants and petroleum-diesel as solvent (PD) for lipid extraction and recovery significantly reduced the energy input at cell wall disruption step. Biodiesel production from scum is a two-step process which is fast and energetically favorable.

Recent developments of downstream processing for microbial lipids and conversion to biodiesel.

With increasing global population and depleting resources, there is an apparent demand for radical unprecedented innovation to satisfy the basal needs of lives. Hence, non-conventional renewable energy resources like biodiesel have been worked out in past few decades. Biofuel (e.g. Biodiesel) serves to be the most sustainable answer to solve "food vs. fuel crisis". In biorefinery process, lipid extraction from oleaginous microbial lipids is an integral part as it facilitates the release of fatty acids. Direct lipid extraction from wet cell-biomass is favorable in comparison to dry-cell biomass because it eliminates the application of expensive dehydration. However, this process is not commercialized yet, instead, it requires intensive research and development in order to establish robust approaches for lipid extraction that can be practically applied on an industrial scale. This review aims for the critical presentation on cell disruption, lipid recovery and purification to support extraction from wet cell-biomass for an efficient transesterification.