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1.
J Biomol Struct Dyn ; 37(18): 4747-4766, 2019 11.
Article in English | MEDLINE | ID: mdl-30580670

ABSTRACT

BCR-ABL fusion protein drives chronic myeloid leukemia (CML) which constitutively activates tyrosine kinase involved in the initiation and maintenance of CML phenotype. Ponatinib, an oral drug, was discovered as an efficient BCR-ABL inhibitor by addressing imatinib drug resistance arising due to the point mutations at its active sites. In this study, 44 BCR-ABL kinase inhibitors, which are derivatives of ponatinib, were used to develop a robust two-dimensional quantitative structure-activity relationship (2D-QSAR) and 3D-Pharmacophore models by dividing dataset into 32 training sets and 12 test set molecules. 2D-QSAR model was developed using Genetic Function Approximation (GFA) algorithm consisting of four types of information-rich molecular descriptors, electrotopological (ES_Count_aasN and ES_Sum_aaaC), electronic (Dipole_X), spatial (PMI_Y) and thermodynamic (LogD), primarily contributing to BCR-ABL kinase inhibitory activity. For the best 2D-QSAR model, the statistics were R2 = 0.8707, R2pred = 0.8142 and N = 32 for the training set molecules. Phase module of Schrödinger suit was employed for 3D-Pharmacophore model development showing five different pharmacophoric features - ADHHPRR with good R2 of 0.9629, F of 175.3, Q2 of 0.645 and root-mean-square error (RMSE) of 0.214 that are essential for an effective BCR-ABL kinase inhibition. These two models were further validated by cross-validation, test set predictions, enrichment factor calculations and predictions based on the external dataset. The molecular mechanism of resistance arising due to gate keeper mutation T315I of ABL kinase in complex with its inhibitors was also studied using molecular docking and molecular dynamics simulations. Our developed models predicted key chemical features for designing potent inhibitors against BCR-ABL kinase activity and its resistance mechanism to CML disease therapy. Communicated by Ramaswamy H. Sarma.


Subject(s)
Antineoplastic Agents/chemistry , Fusion Proteins, bcr-abl/antagonists & inhibitors , Imidazoles/chemistry , Leukemia, Myelogenous, Chronic, BCR-ABL Positive/drug therapy , Protein Kinase Inhibitors/chemistry , Pyridazines/chemistry , Drug Resistance, Neoplasm/genetics , Fusion Proteins, bcr-abl/genetics , Humans , Imidazoles/pharmacology , Leukemia, Myelogenous, Chronic, BCR-ABL Positive/enzymology , Leukemia, Myelogenous, Chronic, BCR-ABL Positive/genetics , Ligands , Molecular Docking Simulation , Molecular Dynamics Simulation , Point Mutation , Pyridazines/pharmacology , Pyrimidines/chemistry , Pyrimidines/pharmacology , Quantitative Structure-Activity Relationship , Thermodynamics
2.
Carbohydr Polym ; 93(2): 661-9, 2013 Apr 02.
Article in English | MEDLINE | ID: mdl-23499109

ABSTRACT

Nanoparticle mediated delivery of antineoplastic agents, functionalized with monoclonal antibodies has achieved extraordinary potential in cancer therapy. The objective of this study was to develop a drug delivery system comprising O-carboxymethyl chitosan (O-CMC) nanoparticles, surface-conjugated with Cetuximab (Cet) for targeted delivery of paclitaxel (PTXL) to Epidermal Growth Factor Receptor (EGFR) over-expressing cancer cells. Nanoparticles around 180±35nm and negatively charged were prepared through simple ionic gelation technique. The alamar blue assay indicated that these targeted nanoparticles displayed a superior anticancer activity compared to non-targeted nanoparticles. The nanoformulation triggered enhanced cell death (confirmed by flow cytometry) due to its higher cellular uptake. The selective uptake of Cet-PTXL-O-CMC nanoparticles by EGFR +VE cancer cells (A549, A431 and SKBR3) compared to EGFR -VE MIAPaCa-2 cells confirms the active targeting and delivery of PTXL via the targeted nanomedicine. Cet-PTXL-O-CMC nanoparticles can be used a promising candidate for the targeted therapy of EGFR over expressing cancers.


Subject(s)
Antibodies, Monoclonal, Humanized/administration & dosage , Chitosan/analogs & derivatives , Drug Delivery Systems/methods , ErbB Receptors/metabolism , Nanoparticles/administration & dosage , Antibodies, Monoclonal, Humanized/chemistry , Antibodies, Monoclonal, Humanized/pharmacology , Antineoplastic Agents/administration & dosage , Biomarkers, Tumor/metabolism , Cell Death , Cell Line, Tumor , Cetuximab , Chemistry, Pharmaceutical/methods , Chitosan/administration & dosage , Drug Screening Assays, Antitumor , Erythrocytes/drug effects , Erythrocytes/ultrastructure , Hemolysis/drug effects , Humans , Microscopy, Electron, Scanning , Nanoparticles/chemistry , Nanotechnology/methods , Oxazines/chemistry , Particle Size , Xanthenes/chemistry
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