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Indian J Med Microbiol ; 32(3): 294-300, 2014.
Article in English | MEDLINE | ID: mdl-25008824

ABSTRACT

PURPOSE: To evaluate the diagnostic utility of ante-mortem tracheal aspirates for diagnosis of ventilator-associated pneumonia (VAP). Trauma victims represent an otherwise healthy population, who are on multiple invasive life-support devices, which predispose them to severe infections like VAP. The diagnosis of VAP is challenging, due to the difficulty in obtaining a representative sample from lungs. We studied the diagnostic utility of tracheal aspirates by comparing its results with the post-mortem lung cultures. MATERIALS AND METHODS: A total of 106 fatal trauma patients were included in the study. Lung samples and cardiac blood were taken for culture at the time of autopsy. The results of ante-mortem and post-mortem cultures were compared. RESULTS: Septicemia was the cause of death in 51 (48%) of the fatal cases and VAP was identified in 36 (34%) cases. A total of 96 (90.5%) cases had pathogens isolated from lung samples. In 62 (58%) cases, the same organism was isolated from ante-mortem and post-mortem respiratory samples. CONCLUSIONS: Culture results of a properly collected tracheal aspirate should be taken into consideration along with Centre for Disease Control and Prevention (CDC's) diagnostic criteria to maximise the diagnosis of VAP.


Subject(s)
Bacteria/classification , Bacteria/isolation & purification , Pneumonia, Bacterial/microbiology , Pneumonia, Ventilator-Associated/diagnosis , Pneumonia, Ventilator-Associated/microbiology , Wounds and Injuries/complications , Wounds and Injuries/therapy , Adolescent , Adult , Aged , Aged, 80 and over , Autopsy , Blood/microbiology , Child , Child, Preschool , Female , Humans , Infant , Lung/microbiology , Male , Middle Aged , Trachea/microbiology , Young Adult
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