Subject(s)
Anopheles/physiology , Anopheles/parasitology , Malaria/transmission , Mosquito Vectors/physiology , Mosquito Vectors/parasitology , Plasmodium falciparum/physiology , Plasmodium vivax/physiology , Animal Distribution , Animals , Asia, Southeastern , Malaria/prevention & control , Mosquito ControlABSTRACT
BACKGROUND & OBJECTIVES: Bio-manipulation technique is of primary importance during the development of transgenic mosquitoes. The study describes the variable factors that influence the viability of medically important mosquito vectors during microinjection. METHODS: Three mosquito vectors belonging to the genus Aedes, Anopheles and Culex were microinjected at different developmental stages of their life cycle viz., egg, larvae, pupae and adult. RESULTS: The improvisations revealed an increased survivability of biomanipulated mosquitoes during the embryonic and adult microinjection. The study of injecting larvae and pupae resulted in poor survivability. INTERPRETATION & CONCLUSIONS: The microinjection protocol was successfully tested on three important mosquito vectors. The critical period after biomanipulation which contributes heavily for the survivability factor was evaluated. The results provide a common protocol for biomanipulation of three mosquito vectors with enhanced survivability.