ABSTRACT
Streptomyces genera serve as adaptable cell factories for secondary metabolites with various and distinctive chemical structures that are relevant to the pharmaceutical industry. Streptomyces' complex life cycle necessitated a variety of tactics to enhance metabolite production. Identification of metabolic pathways, secondary metabolite clusters, and their controls have all been accomplished using genomic methods. Besides this, bioprocess parameters were also optimized for the regulation of morphology. Kinase families were identified as key checkpoints in the metabolic manipulation (DivIVA, Scy, FilP, matAB, and AfsK) and morphology engineering of Streptomyces. This review illustrates the role of different physiological variables during fermentation in the bioeconomy coupled with genome-based molecular characterization of biomolecules responsible for secondary metabolite production at different developmental stages of the Streptomyces life cycle.
Subject(s)
Streptomyces , Streptomyces/genetics , Streptomyces/metabolism , Metabolic Networks and Pathways , GenomicsABSTRACT
The biodegradation of cyclophosphamide and etoposide by Trametes versicolor (AH05), Ganoderma lucidum (MTCC-1039), and Phanerochaete chrysosporium (MTCC-787) were tested for 3, 6, 9, 12, and 15 days, respectively. G. lucidum achieved the highest degradation efficiency of cyclophosphamide (71.5%) and etoposide (98.4%) after 6 days of treatment. The degradation efficiency of T. versicolor and P. chrysosporium for etoposide was 79.8% and 76.8%, respectively. However, no degradation of cyclophosphamide was achieved with P. chrysosporium, although it showed the highest sorption efficiency for cyclophosphamide (23.7%). Trametes versicolor achieved only 1.4% degradation of cyclophosphamide, that includes both biodegradation and biosorption. The pseudo first-order degradation kinetics explained the degradation of etoposide and cyclophosphamide with t1/2 values of 1.32 and 4.43 days and 'k' constant of 0.16 and 0.54 day-1, respectively.
