Development and characterization of active poly (3-hydroxybutyrate) based composites with grapeseed oil and MgO nanoparticles for shelf-life extension of white button mushrooms (Agaricus bisporus).

Poly (3-hydroxybutyrate) (PHB) is undoubtedly a potential substitute for petroleum-based non-biodegradable food packaging materials due to its renewability, high crystallinity, biocompatibility, and biodegradability. Nonetheless, PHB exhibits certain shortcomings, including low flexibility, moderate gas barrier properties, and negligible antimicrobial and antioxidant activities, which limit its direct application in food packaging. Loading essential oils can increase flexibility and induce antimicrobial and antioxidant activities in biopolymers but at the cost of reduced tensile strength. In contrast, nanofiller reinforcement can increase the tensile strength and barrier properties of such biopolymers. Therefore, to harness the synergistic effects of essential oil and nanofiller, PHB-based films incorporated with 5 wt% grapeseed oil (GS) and varying concentrations (0.1-1 wt%) of MgO nanoparticles (MgO NPs) were prepared in this study following simple sonication-assisted solution casting technique. Physicochemical, tensile, microstructural, optical, barrier, antimicrobial, and antioxidant properties were then evaluated for the prepared composite films. FESEM analysis of the PHB-based films with 5 wt% GS and 0.7 wt% MgO NPs (PHB/5GS/0.7MgO) confirmed its compact morphology without any aggregates, pores, or phase separation. In comparison with pristine PHB, the PHB/5GS/0.7MgO films demonstrated higher tensile strength (by 1.4-fold) and flexibility (by 30-fold), along with 79 and 90 % reduction in water vapor and oxygen transmission, respectively. In addition, PHB/5GS/0.7MgO showed good UV-blocking properties, 65.25 ± 0.98 % antioxidant activity, and completely inhibited the growth of Staphylococcus aureus and Escherichia coli. Moreover, PHB/5GS/0.7MgO films proved beneficial effects in terms of extending the shelf-life of white button mushrooms up to 6 days at ambient room conditions.

Facile fabrication and characterization of novel antimicrobial and antioxidant poly (3-hydroxybutyrate)/essential oil composites for potential use in active food packaging applications.

Poly (3-hydroxybutyrate) (PHB) is a bio-based biodegradable biopolymer with excellent potential to substitute petrochemical-based food packaging materials. Nevertheless, low elongation at break is one of the limiting factors for its commercial-scale application in the packaging field. Microbial contamination and lipid oxidation are the two main causes of food spoilage and pose huge challenges to the food industry. In this regard, essential oils are bioactive compounds that, in addition to providing antimicrobial and antioxidant properties, can improve the flexibility of biopolymers. Therefore, to overcome the aforementioned challenges, the current study aimed to fabricate novel PHB composite films loaded with essential oils, viz. grapeseed oil (GS), bergamot oil (BG), and ginger oil (GG), by a simple solution casting technique. To evaluate the potential of prepared PHB/essential oil composites for food packaging applications, extensive characterizations of their mechanical, structural, barrier, optical, and thermal properties were carried out. Interestingly, PHB/essential oil composites demonstrated good UV-blocking properties without affecting its transparency. PHB films loaded with 5 wt% GS showed a 30-fold enhancement in flexibility compared to pristine PHB. The DPPH radical scavenging activities of PHB/5GS, PHB/5BG, and PHB/5GG films are 53.17 ± 4.76, 50.70 ± 3.92 and 86.38 ± 2.73 %, respectively. The antibacterial activities of PHB/5GS, PHB/5BG, and PHB/5GG films against the model bacterium E. coli are 19.72 ± 0.97, 12.62 ± 2.23 and 29.98 ± 2.15 %, respectively, whereas, for S. aureus, the values are 61.56 ± 3.39, 30.28 ± 0.92 and 70.97 ± 0.26 %, respectively. Moreover, the overall migration values of the composite films in simulants representing hydrophilic, acidic, and lipophilic foods did not exceed the prescribed overall migration limit (10 mg/dm2).

Recent advances and future prospects of cellulose, starch, chitosan, polylactic acid and polyhydroxyalkanoates for sustainable food packaging applications.

Cellulose, starch, chitosan, polylactic acid, and polyhydroxyalkanoates are seen as promising alternatives to conventional plastics in food packaging. However, the application of these biopolymers in the food packaging industry on a commercial scale is limited due to their poor performance and processing characteristics and high production cost. This review aims to provide an insight into the recent advances in research that address these limitations. Loading of nanofillers into polymer matrix could improve thermal, mechanical, and barrier properties of biopolymers. Blending of biopolymers also offers the possibility of acquiring newer materials with desired characteristics. However, nanofillers tend to agglomerate when loaded above an optimum level in the polymer matrix. This article throws light on different methods adopted by researchers to achieve uniform dispersion of nanofillers in bionanocomposites. Furthermore, different processing methods available for converting biopolymers into different packaging forms are discussed. In addition, the potential utilization of agricultural, brewery, and industrial wastes as feedstock for the production of biopolymers, and integrated biorefinery concept that not only keep the total production cost of biopolymers low but are also environment-friendly, are discussed. Finally, future research prospects in this field and the possible contribution of biopolymers to sustainable development are presented. This review will certainly be helpful to researchers working on sustainable food packaging, and companies exploring pilot projects to scale up biopolymer production for industrial applications.