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Biosci Biotechnol Biochem ; 78(1): 60-6, 2014.
Article in English | MEDLINE | ID: mdl-25036484

ABSTRACT

Ascorbate (AsA) is an important antioxidant and an enzyme cofactor involved in various metabolic pathways. In this study, we investigated the effects of estrogen (ES)-inducible transient expression of genes encoding enzymes involved in the d-mannose/l-galactose (d-Man/l-Gal) pathway for plant AsA biosynthesis on AsA levels under light and dark conditions. No significant difference was observed in AsA levels between Arabidopsis plants transiently expressing phosphomannose isomerase (PMI1), GDP-d-Man pyrophosphorylase (GMP/VTC1), GDP-Man-3',5'-epimerase (GME), and l-Gal 1-phosphate phosphatase (GPP/VTC4), but AsA levels in the plants transiently expressing GDP-l-Gal phosphorylase (GGP/VTC2) were 2.5-fold higher than those in control plants 7 d after ES treatment. The increase in AsA levels under continuous light conditions and the decrease in AsA levels under dark conditions were enhanced and suppressed, respectively, in the ES-treated plants. These results suggest that GGP/VTC2 acts as a rate-limiting step regulating AsA biosynthesis in response to light and dark conditions.


Subject(s)
Arabidopsis Proteins/genetics , Arabidopsis/genetics , Arabidopsis/metabolism , Ascorbic Acid/biosynthesis , Darkness , Gene Expression Regulation, Plant , Phosphoric Monoester Hydrolases/genetics , Arabidopsis/enzymology , Arabidopsis/radiation effects , Arabidopsis Proteins/metabolism , Ascorbic Acid/metabolism , Biocatalysis , Estrogens/pharmacology , Gene Expression Regulation, Plant/drug effects , Gene Expression Regulation, Plant/radiation effects , Intracellular Space/drug effects , Intracellular Space/metabolism , Intracellular Space/radiation effects , Phosphoric Monoester Hydrolases/metabolism
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