[Characterization of smooth muscle organs by determination of cellular myosin content]. / Charakterisierung glattmuskulärer Organe mit Hilfe der Bestimmung des Myosingehaltes der Zellen.

The mean relative content of myosin of cells from smooth muscular organs was estimated using immunocytochemical methods after enzymatical tissue disintegration. The cells were classified according to myosin content. Aortas and urinary bladders of newborn rats and chicken amnions were used as models. Cells of a permanent cell strain, being free of smooth muscular myosin served as negative controls. It could be shown, that the content of myosin, estimated by immunocytochemical methods correlates with the state of development of smooth muscular organs.

[Changes in the amount of myosin and the rate of myosin biosynthesis in vascular cells in culture]. / Anderungen des Myosingehaltes und der Myosinbiosyntheserate kultivierter Gefässzellen.

The relative myosin content of aortic cells of newborn rats was estimated cytophotometrically in the course of cultivation using indirect immunofluorescence staining. It should be shown that the average myosin content of the cell population and the number of cells with high myosin content is greatly enhanced when confluency of the cells is reached and proliferation stops. Following the amount of 14C-labelled leucine incorporated in immunoprecipitated myosin during the same period we found that increasing myosin content of the cells results from an enhanced rate of myosin biosynthesis.

[Investigation of in vitro cultivated cells from smooth muscular organs. 5. Immunohistochemical experiments for demonstration of myosin and estimation of changes in the myosin content in the aorta and in isolated respectively cultivated aortic cells of newborn rats (author's transl)]. / Untersuchungen in vitro kultivierter Zellen glattmuskulärer Organe 5. Mitteilung: Immunhistochemische Untersuchungen zum Nachweis von Myosin und zur Erfassung von Anderungen des Myosingehaltes in der Aorta sowie in isolierten oder kultivierten Aortenzellen neonataler Ratten.

Smooth muscle myosin was demonstrated in cryostat sections, isolated and cell cultures from aortas of newborn rats by the method of indirect immunofluorescence. The content of myosin in the cells was compared by measuring the fluorescence intensity of single cells. Well differentiated smooth muscle cells present in suspensions of the whole aorta and of the aortic media as well as in aortic cell cultures, are shown to have a higher myosin content than fibroblasts of adventitia and smooth muscle cells with a lower degree of differentiation.