ABSTRACT
The main aim of this research was to establish the nucleotide sequence of the highly variable region of the D loop of the mitochondrial DNA of some Georgian domestic animal species (cattle, goat, sheep) as well as their phylogenetic position among the worldwide set of domestic animals. In this study, a total of 5 haplogroups (T - 5; T3 - 7; T1 - 1; T2 - 2; T5 - 2) in 17 Georgian Mountain cattle (GMC), 4 haplogroups (A - 15; A2a1 - 3; A1a - 1; A6 - 3) in 22 Georgian goats and 3 haplogroups (A - 10; B - 16; C -15) in 41 Georgian sheeps (15 Imeretian and 26 Tushetian) were detected. This study represents the first attempt of Genetic study of native Georgian livestocks. The GMC, Georgian (Megrelian) goat, Georgian (Imeretian and Tushetian) sheep mitogenomes were grouped phylogenetically in the haplogroups indicating the closeness to the Near Eastern animals.
ABSTRACT
There is strong evidence that a restricted part of the chick forebrain, the intermediate medial mesopallium (IMM), stores information acquired through the learning process of visual imprinting. We have previously demonstrated that at 1 h but not 24 h after imprinting training, a learning-specific increase in the amount of membrane Thr286-autophosphorylated α-calcium/calmodulin-dependent protein kinase II (αCaMKII), and in the proportion of total αCaMKII that is phosphorylated, occurs in the IMM but not in a control brain region, the posterior pole of the nidopallium (PPN). αCaMKII directly phosphorylates Ser831 in the GluA1 subunit of the α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor. In the present study we have inquired whether the learning-related increase in αCaMKII autophosphorylation is followed by changes in the Ser831 phosphorylation of GluA1 (P-GluA1) and in the total amount of this subunit (T-GluA1). Trained chicks together with untrained control chicks were killed either 1 or 24 h after training. Tissue was removed from the IMM together with tissue from the PPN as a control. Amounts of P-GluA1 and T-GluA1 were measured. In the left IMM of the 1 h group the P-GluA1/T-GluA1 ratio increased in a learning-specific way. No learning-related changes were observed in other brain regions at 1 h or in any region 24 h after training. The results indicate that a time- and regionally-dependent, learning-specific increase in GluA1 phosphorylation occurs early in recognition memory formation.