ABSTRACT
A new peptide construct Palm135-158-GGA-170-188(Acm) has been synthesized and investigated in a number of in vitro and in vivo test systems. The construct contains a virus specific T-helper epitope within the 170-188 sequence of VP1, in addition to the main antigenic 135-158 region of the foot-and-mouth disease viral VP1 protein (strain A22). The construct has higher protective, antigenic, immunogenic and T-cell proliferative activity then the previously described shorter peptide Palm(2)135-159. The 170-188 part of the construct serves as a virus specific T-epitope, responsible for the enhanced immunogenic and protective activity of the construct.
Subject(s)
Aphthovirus/immunology , Epitopes, B-Lymphocyte , Epitopes, T-Lymphocyte , Peptide Fragments/immunology , Vaccines, Synthetic/immunology , Viral Vaccines/immunology , Amino Acid Sequence , Animals , Antibodies, Viral/blood , Capsid/immunology , Capsid Proteins , Cattle , Female , Guinea Pigs , Lymphocyte Activation , Mice , Mice, Inbred Strains , Molecular Sequence Data , Rabbits , Sheep , T-Lymphocytes/immunologyABSTRACT
The peptide Palm2 135-159, a dipalmitoyl derivative of the 135-159 fragment of VP1 protein of the foot-and-mouth disease virus strain A22 was synthesized. In the experiments on mice, guinea pigs and sheep Palm2 135-159 possesses greater immunogenic and protective activity than the nonacylated 135-159 peptide. The synthetic vaccine against foot-and-mouth disease for use in sheep was developed on the basis of the lipopeptide. Synthetic polymethylsiloxane oil was found to be a suitable adjuvant for this vaccine. The dependencies of protective and immunogenic effects from the dose of peptide were studied. The vaccine was found to be stable to storage for 1 year at 18 degrees C. It was shown that the synthetic vaccine provides 1 year protection of sheep against foot-and-mouth disease after a single administration. The vaccine is allowed for veterinary use in Russia.
