[Detection of some markers of the early phase of the inflammatory and reparative process in mechanical skin injuries]. / Vyyavlenie nekotorykh markerov rannei fazy vospalitel'no-reparativnogo protsessa pri mekhanicheskikh povrezhdeniyakh kozhi.

OBJECTIVE: To examine the applicability of IHC staining method: with TGF-ß1 antibodies (serial examination, statistically processed results) and with mast cell tryptase antibodies for injuries vitality determination. MATERIAL AND METHODS: 261 skin autopsy samples with mechanical injuries from 29 persons were divided to 3 groups (87 in each group): vital injuries, postmortal injuries, control non-injured samples. A routine histological examination using standard H&E stain and IHC both with TGF-ß1 and mast cells tryptase antibodies was performed. RESULTS AND CONCLUSION: The positive TGF-ß1 staining (score 2-3) was found in keratinocytes in vitally injured skin and the negative or weak one (score 0-1) was found in control postmortally injured and non-injured samples. Additionally, dermal TGF-ß1 expression was found in some vitally injured skin samples. The difference between vitally injured skin and control samples was statistically significant (p<0.05). No significant difference of dermal mast cells density in groups 1, 2, 3 was found.

[TGFß1 antibodies application for IHC determination of injuries vitality. The experimental results]. / Rezul'taty eksperimental'nogo primeneniya antitel k TGFß1 dlya immunogistokhimicheskoi otsenki prizhiznennosti povrezhdenii.

THE AIM OF THE STUDY: Is to examine the applicability of IHC staining method with TGFß1 antibodies for injuries vitality determination. 72 skin autopsy samples with mechanical injuries from 12 persons were divided to 3 groups (n=24 in each group): vital injuries, postmortal injuries, control non-injured samples. A routine histological examination using standard H&E stain and IHC with TGFß1 antibodies was performed. We concluded the positive TGFß1 staining was in keratinocytes in vitally injured skin and the negative one in postmortally injured and control non-injured samples. The role of TGFß1 in the injuries healing process and the further examinations of the TGFß1 staining method application for determination of injuries vitality are discussed.

[Forensic pathological diagnostics of rare T-cell leukemia in re-examination]. / Sudebno-gistologicheskaya diagnostika redkogo T-kletochnogo leikoza pri proizvodstve povtornoi ekspertizy.

A forensic pathological case of rare T-cell leukemia is reported. Autopsy samples (paraffine blocks and slides) from 32 years old female with long-term existing anemia and leukopenia, number of chronic inflammatory diseases, died from fulminant pneumonia, were presented for forensic pathological re-examination. A routine histological examination was done using standard H&E stain. Additionally, we used IHC stain with antibodies to CD3 and CD20 when examining bone marrow and liver samples. The T-cell infiltration in bone marrow (interstitial and intrasinusoidal type) were found. The next step was IHC bone marrow examination with CD4, CD5, CD8, CD56 antibodies. We concluded that the patient had unrecognized T-cell leukemia, based on anamnestical, clinical data and results of our histological and IHC examination. In this case, the T-cell leukemia was not found neither vitally nor at primary forensic pathological examination after autopsy. The necessity of minimal IHC stain sets usage in primary forensic pathology units and IHC's decisive role in bone marrow pathological examination are discussed.

[Role of Kraevsky cells in forensic histological diagnostics]. / Znachenie kletok Kraevskogo v sudebno-gistologicheskoi diagnostike.

The study objective is to establish the role of light hepatocytes (Kraevsky cells) for forensic histological diagnosis of glycogen depletion in the liver due to various conditions, including shock. A standard histological examination of liver samples of persons who died from various causes was performed; routine staining with hematoxylin and eosin and additional staining by the PAS method was used. Histotopographic comparison of cross sections was performed to clarify the localization of light hepatocytes with different PAS staining results. It was shown that light hepatocytes have different morphological features, localization, and results of PAS staining, regardless of the cause of death, and are not related to glycogen depletion in them. Light hepatocytes (Kraevski cells) cannot be used as a sign of glycogen depletion and/or shock.

[Reliability of the immunohistochemical estimation of vitality and injury age: analysis and perspectives for study]. / Dostovernost' immunogistokhimicheskoi otsenki prizhiznennosti i davnosti povrezhdenii: analiz i perspektivy issledovanii.

This review summarizes the literature data on the applicability of various immunohistochemical markers for the diagnosis of vitality and injury age. Current state of the problem of the reliability of the estimation of injury vitality, and the most promising immunohistochemical markers for further study are also considered.