Subject(s)
Intestinal Absorption/drug effects , Intestine, Large/drug effects , Peptides/pharmacology , Animals , Epithelium/drug effects , Epithelium/physiology , In Vitro Techniques , Intestinal Absorption/physiology , Intestine, Large/physiology , Membrane Potentials/drug effects , Membrane Potentials/physiology , Rana temporaria , Time FactorsABSTRACT
I.V. Infusion of bombesine after eating raw meat inhibited for 30-60 min the secretion of gastric juice and hydrochloric acid in dogs. Within 90-120 min of simultaneous infusion of pentagastrin and bombesine, the amount of secreted juice and its acidity decreased and then the secretion of gastric parietal cells increased. Simultaneous infusion of histamine and bombesine increased the response of gastric parietal cells during the whole experiment as compared with the histamine effect alone. Microapplication of bombesine into cerebral structures also decreased the secretory response of the parietal cells.
Subject(s)
Bombesin/analogs & derivatives , Bombesin/pharmacology , Gastric Acid/metabolism , Gastric Juice/drug effects , Pepsin A/drug effects , Animals , Dogs , Dose-Response Relationship, Drug , Gastric Acidity Determination , Gastric Juice/chemistry , Gastric Juice/metabolism , Histamine/pharmacology , Meat , Parietal Cells, Gastric/drug effects , Parietal Cells, Gastric/metabolism , Pentagastrin/pharmacology , Pepsin A/analysis , Pepsin A/metabolism , Stimulation, Chemical , Time FactorsABSTRACT
A convenient route of synthesis of amphibian bombesin and bombesin-like peptide alytesin was found. These tetradecapeptides were obtained by assembling the 1-5 and 6-14 fragments by means of DCC-HONB or mixed anhydrides methods. Structure of the tetradecapeptides was confirmed by high resolution NMR spectroscopy data. The bombesin and alytesin synthesized potently decrease body temperature and stimulate pancreatic juice secretion.
Subject(s)
Bombesin/chemical synthesis , Peptides/chemical synthesis , Amino Acid Sequence , Animals , Anura , Chromatography, Ion Exchange , Magnetic Resonance Spectroscopy , Molecular Sequence DataSubject(s)
Bombesin/physiology , Brain/physiology , Receptors, Neurotransmitter/physiology , Animals , Blood-Brain Barrier/drug effects , Body Temperature Regulation/drug effects , Bombesin/pharmacology , Brain/drug effects , Heart Rate/drug effects , Humans , Receptors, Bombesin , Receptors, Neurotransmitter/drug effectsSubject(s)
Body Temperature Regulation/drug effects , Bombesin/pharmacology , Animals , Male , RabbitsABSTRACT
Central or peripheral administration of bombesin--tetradecapeptide obtained from amphibian skin, acted upon the nervous system, behaviour, thermoregulation and gastro-intestinal tract. New experimental data on synthesis, immunohistochemistry and physiological effects of bombesin and related peptides are presented.
Subject(s)
Bombesin/metabolism , Nerve Tissue Proteins/physiology , Peptides/metabolism , Animals , Appetite/physiology , Body Temperature Regulation , Bombesin/analysis , Central Nervous System/physiology , Digestion , Digestive System Physiological Phenomena , Dogs , Gastric Juice/metabolism , Insulin/metabolism , Insulin Secretion , Islets of Langerhans/physiology , Mice , Motor Neurons/physiology , Muscle, Smooth, Vascular/physiology , Pancreas/physiology , Pituitary Gland/physiology , Pituitary Hormones/metabolism , Rats , Renin/metabolismSubject(s)
Bicarbonates/metabolism , Bombesin/pharmacology , Pancreas/metabolism , Pancreatic Juice/metabolism , Peptide Fragments/pharmacology , Peptides/pharmacology , Animals , Bombesin/administration & dosage , Dogs , Injections, Intramuscular , Pancreas/drug effects , Peptide Fragments/administration & dosageABSTRACT
Out of synthetic tetradecapeptide bombesin, its fragments C-terminal nonapeptide and N-terminal pentapeptide and their analogues ( Val7 , D- Ala11 ), ( Ala7 , D- Ala11 ), (Leu7, D- Ala11 ) and ( Pro6 , Gly7 , D- Ala11 ), the first two, being injected into the lateral ventricle of the brain in rabbits, affected the body temperature in cold environment (+ 10 degrees C). The analogues exerted a lesser effect on the body temperature. N-terminal pentapeptide was ineffective both in cold (+ 10 degrees C) and warm (+ 30 degrees C) environment.
