1.
Leg Med (Tokyo)
; 11 Suppl 1: S466-7, 2009 Apr.
Article
in English
| MEDLINE
| ID: mdl-19299190
ABSTRACT
Aware that long-term storage bloodstain generally reduces numbers of detectable loci, we performed DNA typing with bloodstains stored at room temperature for 22-30years, then extracted DNA from the bloodstains using the AmpFlSTR Y-filer PCR Amplification Kit. We performed electrophoresis with an ABI 310 Genetic Analyzer and determined alleles using GeneMapper ID v3.2 software. Our results suggest that Y-filer finds certain loci more difficult to anneal than others.