Isolated hepatocytes stimulation activity on toxic liver failure in experimental study.

Finding of affective treatment methods of acute liver failure (alf), consists one of difficult solving problem in modern hepatology. To day improving liver transplantation methods for the stimulation of regeneration process. Transplantation of liver allogenic isolated hepatocytes in A.L.F.treatment, promotes regeneration morphological capabilities of toxically damaged liver. We used combined enzymatic-mechanical method modified by Korukhov (1983), which let to take up 50-60% of cells from liver mass, 80% from them maintained their morphological structure and functional activity. Experiments were performed on 45 white laboratory rats. Animals were divided into 3 groups. I group of animals (n=20) was the control group, with acute liver failure without treatment, in this group, lethal outcome was 100% in the I group, after CCL4 administration on 5 and 7 days. II group (n=20) included animals who had ALF model and which provides treatment with isolated hepatocytes transplantation. III group of animals were served as donors for isolated hepatocytes uptake. 90% of animals from II group were alive after treatment during 20 days and then their condition was satisfactory. Biochemical and morphological investigation proved that our treatment method induces toxically damaged liver function restoration.

Toxic acute liver failure treatment by isolated hepatocytes allotransplantation in experimental.

Acute liver failure treatment nowadays is a common problem in modern hepatology, despite of well studied pathogenesis and treatment modalities, lethality of the disease is still high, and in 21 century in the world's best clinics it accounts to 70-80%. For uptake of isolated hepatocytes we uses the fermental-mechanical method modified by Korukhov (1983) for the treatment of acute liver failure by hepatocyte allotransplantation and to stimulate liver reparative regeneration processes, which gives a chance to liver for the function restoration by replacement of injured hepatocytes with transplanted liver cells. We provide experiments on 45 white laboratory Vistar line rats (150-200gr) which were kept on standard environment, animals were divided into three equal groups: I group (n=20) was served as a control group, in which we provide acute liver failure modelling, by toxic agent (carbontetrachloride CCL4 ) treatment by naso-gastric tube, After acute liver failure modelling, in the II group (n=20) of animals we were conducting liver cell transplantation, III group (n=5) animals were served as donors for isolated hepatocyte uptake. Cell ultrastructure element proliferation clearly seen by morphological evaluation and animals which have acute liver failure had a chance for survival and it may be concluded that our proposed method can be the curative treatment method of acute liver failure in experiment.