ABSTRACT
The effect of peptide Semax on remodeling of cardiac sympathetic innervation was examined in rats with experimental myocardial infarction. In 28 days after ischemia/reperfusion injury, Semax diminished the growth of sympathetic innervation of ventricular septum, although it produced no effect on the density of ß1 and ß2 adrenoceptors.
Subject(s)
Myocardial Infarction/metabolism , Prostatitis/metabolism , Adrenocorticotropic Hormone/analogs & derivatives , Adrenocorticotropic Hormone/therapeutic use , Animals , Male , Myocardial Infarction/drug therapy , Peptide Fragments/therapeutic use , Prostate/drug effects , Prostate/metabolism , Prostate/pathology , Prostatitis/drug therapy , Quercetin/analogs & derivatives , Quercetin/therapeutic use , Rats , Rats, Wistar , Sympathetic Nervous System/drug effects , Sympathetic Nervous System/metabolism , Sympathetic Nervous System/pathologyABSTRACT
Tripeptide glycyl-prolyl-proline (PGP), a regulatory peptide of the glyproline family, possesses a pronounced anti-inflammatory effect primarily due to its ability to prevent secretion of the proinflammatory mediator histamine by rat peritoneal mast cells. Activation of mast cell with synacthen (ACTH1-24) and substance 48/80 leads to an increase in intracellular calcium concentration. Pretreatment of mast cells with PGP prevented calcium entry into the cytoplasm from both intercellular space and intracellular stores. Acetylated peptide (N-AcPGP) produced a similar effect on histamine release and intracellular calcium content in mast cells activated with synacthen. These findings indicate that both forms of the peptide can stabilize mast cells and prevent intracellular calcium increase.
Subject(s)
Calcium/metabolism , Cytoplasm/drug effects , Mast Cells/drug effects , Oligopeptides/pharmacology , Proline/analogs & derivatives , Animals , Cells, Cultured , Cytoplasm/metabolism , Male , Mast Cells/metabolism , Oligopeptides/chemistry , Peritoneal Cavity/cytology , Proline/chemistry , Proline/pharmacology , RatsABSTRACT
This study is dedicated to the research of the regulatory peptide PGP and its acetylated form (N-AcPGP) effect on the paw edema formation and vascular permeability in rat skin. Edema was induced by subcutaneous administration of histamine. Vascular permeability was determined by intradermal injection of mast cells activators corticotropin-releasing hormone (CRH), lipopoly-saccharide (LPS), Synacthen (corticotropin analogue), histamine and compound 48/80. We established that PGP reduced the size of the paw edema, but N-AcPGP had an opposite effect increasing paw edema. Skin vascular permeability didn't increase in rats under the administration of PGP or N-AcPGP with additional injections of CRH, LPS and Synacthen, but with additional injections of histamine and substance 48/80. We demonstrated in vitro that pretreatment with both PGP and N-AcPGP reduced histamine secretion by rat's peritoneal mast cells under activation by Synacthen. These results provide evidence that the effect of the peptides on vascular permeability is mainly mediated by the influence on the secretory activity of mast cells.
