ABSTRACT
Activation of herpes infection in keratoplasty was studied in 26 patients and found to be caused both by operative intervention and the corresponding corticosteroid therapy. The fluorescent antibody technique proved to be the most effective and rapid test for the detection of herpes infection. It is recommended to carry out laboratory control in transplantation of the cornea as well as topical antiviral therapy in the postoperation period not only in patients with herpetic keratitis but also in the subjects operated on for nonspecific leukoma and other diseases of the cornea.
Subject(s)
Corneal Transplantation , Keratitis, Dendritic/transmission , Surgical Wound Infection/transmission , Adrenal Cortex Hormones/adverse effects , Antibodies, Viral/analysis , Antigens, Viral/analysis , Fluorescent Antibody Technique , Hemagglutination Tests , Humans , Keratitis, Dendritic/etiology , Keratitis, Dendritic/immunology , Simplexvirus/immunology , Surgical Wound Infection/etiology , Surgical Wound Infection/immunologyABSTRACT
The duration and pattern of the course of herpes virus infection of the eye and the method used were shown to influence the effectiveness of the laboratory diagnosis. In the first two weeks of the disease conjunctival scrappings should be examined by immunofluorescence and virus isolation by intracerebral inoculation of mice, at later periods by immunofluorescence of conjunctival scrappings or infected cell cultures. Isolated herpes uveitis should be diagnosed by demonstration of viremia. In surgical treatment of herpetic eye diseases virus should be demasked from the cornea by cocultivation of its fragments with human embryo fibroblast culture.