[Lysine overproduction mutations in the yeast Saccharomyces cerevisiae and its transfection into industrial Yeast strains ]. / Mutatsii, vyzyvaiushchie sverkhproduktsiiu lizina u drozhzhei Saccharomyces cerevisiae, i ikh vvedenie v proizvodstvennye shtammy.

Yeast mutants resistant to a toxic lysine analog, thialysine were obtained by a method described in the literature. A strain excreting the maximum amount of lysine (0.45 g/l) was selected from these mutants. The intracellular content of lysine was also increased by 30%. The genetic nature of lysine overproduction was studied in this strain. An increase in the amount of excreted lysine was shown to be determined by at least two genes, one of which carries a mutation of thialysine resistance manifesting the pleiotropic effect of lysine overproduction (Th1R) and the other is involved in the regulation of lysine production (PRL). Linkage groups of these genes were determined: the first gene was mapped to the IV chromosome and the second, to the XV chromosome. Both genetic characters were introduced into industrial baker's yeast strains via a series of backcrosses. The stabilization of the genome in the newly derived strains was confirmed by electrokaryotyping.

[A toxicological study of the preparation Phytomix-40]. / Toksikologicheskoe izuchenie preparata Fitomiks-40.

The results of toxicological evaluation of the new plant preparation Phitomix-40 (PM-40) used in the treatment of cancer are presented. To prevent a patient's individual immunological responsiveness to some phytoadaptogens, the preparation comprises 40 plant extracts (Rhodiola r. L.; Juniperus C. L.; Helichrysum a. L; Viburnum o. L., etc.). Animal experiments demonstrated that PM-40 had a low toxicity; LD50 was more than 20 and 15 ml/kg for rats and mice, respectively.

[Specificity of replicating instability in Schizosaccharomyces pombe haploid yeasts]. / O spetsifichnosti replitsiruiushcheisia nestabil'nosti u drozhzhei Schizosaccharomyces pombe.

UV-induced genetic instability in haploid Schizosaccharomyces pombe does not appear to be very locus-specific. This conclusion contradicts the data previously published by other authors. The possible causes for this discrepancy are discussed.