ABSTRACT
1. Strain differences of mice were found in the taste synergism between monosodium L-glutamate (MSG) and disodium 5'-guanylate (GMP). 2. Magnitudes of chorda tympani responses to the mixture of MSG and GMP over the sum of responses to each component were greater in the order of C3H/HeSlc(C3H) greater than C57BL/6CrSlc(C57BL) greater than BALB/cCrSlc(BALB) mice. The greatest synergism was observed in response to the mixture of 0.03 M MSG and 0.1 mM GMP, to which responses were about 2.6, 1.8 and 1.4 times greater than the sum of each component in C3H, C57BL and BALB mice, respectively. 3. Magnitudes of inhibition of MSG and mixture responses by the lingual treatment of proteolytic enzyme, Pronase E, were greater in the same order of C3H greater than C57BL greater than BALB mice as that observed in magnitudes of the synergism. These results suggest that there exists quantitative differences in receptors responsible for taste synergism between MSG and GMP among three mouse strains.
Subject(s)
Chorda Tympani Nerve/physiology , Guanosine Monophosphate/pharmacology , Sodium Glutamate/pharmacology , Taste/physiology , Animals , Drug Synergism , Electrophysiology , Genetic Variation , Male , Mice , Mice, Inbred BALB C , Mice, Inbred C3H , Mice, Inbred C57BL , Taste/geneticsABSTRACT
The induction of mutation by cadmium chloride (CdCl2), platinum tetrachloride (PtCl4), and rhodium trichloride (RhCl3.3H2O) was measured utilizing the hypoxanthine-guanine phosphoribosyl transferase (HGPRT) locus in V-79 cells. Cadmium chloride at a concentration of 0.05 microM induced ford mutation in V-79 cells 4 times that of their control mutation frequency. Platinum tetrachloride at a concentration of 15 microM and 300 microM rhodium trichloride also induced ford mutation in V-79 cells at rates of around 7 and 4 times control rates, respectively. Platinum is often, and rhodium sometimes added to dental metallic alloys to improve their metallurgic characteristics and cadmium has been mingled in such alloys as well. Moreover, metallic dental drugs such as K2PtCl4 are used a dentine desensitizers. Thus, the genetic hazards of metal ions including cadmium, platinum, and rhodium that dissolve from dental materials and drugs must be investigated and caution exercised for their safe use in dental clinics.
Subject(s)
Biocompatible Materials , Cadmium/toxicity , Dental Materials/toxicity , Mutagens , Platinum Compounds , Platinum/toxicity , Rhodium/toxicity , Animals , Cadmium Chloride , Cells, Cultured , Cricetinae , Cricetulus , MutagenesisABSTRACT
The cytotoxic effects of an oxide-anodized titanium alloy were investigated by cell and organic culture studies. Toxicity was estimated based on cell survival and growth rates compared with those of control materials (glass) after cultivation of cells (L 929) and chicken embryonic femurs expose to alloy plates. The oxide-anodized titanium alloy showed no cytotoxicity with respect to survival or growth rates in either cells or chicken embryonic femurs. Furthermore, the oxide-anodized titanium alloy was more acid and alkali resistant than intact titanium alloys. The results suggest that oxide-anodized titanium has greater stability and its metallic ions may be leachedless under various conditions following dental implantations. Therefore, this alloy should be suitable for use as a dental implant material.
Subject(s)
Dental Alloys/toxicity , Titanium/toxicity , Aluminum , Animals , Biocompatible Materials , Cells, Cultured , Chick Embryo , Cobalt/toxicity , Dental Implants , Mice , OxidesABSTRACT
Thyroglossal duct cyst has been reported to be derived from epithelial rests in the remnants of the thyroglossal duct produced by the descendant of the thyroid anlage. A 34-year-old man consulted our clinic with complaints of painless swelling located in the median cervical region. This tumor was excised under general anesthesia, according to the Sistrunk method. Histopathological findings showed that the inner side of the cyst wall was covered with the stratified squamous epithelium and cliniated columnar epithelium, and the outer layer, thickened connective tissue. No complications or recurrence occurred 2 years and half after the operation.
Subject(s)
Thyroglossal Cyst/surgery , Adult , Humans , MaleABSTRACT
8 cases of branchial cleft cysts and 1 case of branchiogenic carcinoma were examined immunohistochemically for detectable keratin, immunoglobulins (IgG, IgA, IgM), carcinoembryonic antigen (CEA), factor VIII related antigen (Factor VIII RGA), and lysozyme. Lectin binding patterns were also determined. Histologically, cystic lining epithelia were classified into stratified squamous epithelium without keratinization, columnar epithelium with or without cilia, or a mixture of both. Almost all of the cases indicated accompanying lymphoid structures with germinal centers. Keratin expression in epithelial cells was slightly positive, and lectin binding affinities in them were similar to those of oral squamous epithelium. CEA was found on the surface border of columnar epithelial cells, but cystic epithelia in most of the cases were devoid of lysozyme. Endothelial cells of capillary vessels showed positive binding by UEA-1 lectin and the presence of factor VIII RAG. In the lymphoid structures, there were scattered strongly positive lysozyme-staining cells as well as a few lymphocytes bearing IgG, IgA, or IgM.
Subject(s)
Branchial Region/pathology , Cysts/pathology , Adult , Antigens/analysis , Carbohydrates/analysis , Carcinoembryonic Antigen/analysis , Factor VIII/analysis , Factor VIII/immunology , Female , Humans , Immunoglobulins/analysis , Immunohistochemistry , Keratins/analysis , Lactoferrin/analysis , Lectins , Male , Middle Aged , Muramidase/analysis , von Willebrand Factor/analysisABSTRACT
In the present study a Hotz type orthopedic plate was used to improve feeding and physiological growth. In 2 patients with cleft lip it was used to improve feeding and swallowing. In 4 patients with cleft lip and palate it was used to normalize functions and aid physiological growth. The effects of the Hotz orthopedic plate were determined by recording body weight growth, milk volume per day, feeding time and comparison of the study model between before and after using Hotz orthopedic plate. Good physiological growth and improved feeding, were obtoincdinalmostall the patients who wore the Hotz type orthopedic plate correctly.
