ABSTRACT
The effect of ribonuclease and cytochrome c on electrophoretic mobility of liposomes composed of phosphatidylcholine and diphosphatidylglycerol has been studied. zeta-Potential of lipid vesicles was found to decrease in the presence of proteins. Parameters of proteins binding to phospholipids were evaluated from the changes of surface charge density of model membranes. The constants of protein association with phospholipids were calculated to be about 4 x 10(4) M-1 for ribonuclease and about 5.4 x 10(4) M-1 for cytochrome c.
Subject(s)
Cytochrome c Group/chemistry , Liposomes/chemistry , Ribonucleases/chemistry , Animals , Cattle , Electrophoresis, Polyacrylamide GelABSTRACT
The effect of triton X-100 and glutaraldehyde on the functioning of microsomal oxidoreductases has been investigated. Triton X-100 was found to increase the enzymes activity. The decrease of oxidoreductases activity was observed in the presence of glutaraldehyde.
Subject(s)
Glutaral/pharmacology , Intracellular Membranes/drug effects , Microsomes, Liver/drug effects , Octoxynol/pharmacology , Oxidoreductases/drug effects , Animals , Detergents , Microsomes, Liver/enzymology , Microsomes, Liver/ultrastructure , RabbitsABSTRACT
The effect of electrons with energy 5 MeV on the functional state of rabbit liver microsome oxidoreductases has been investigated. Decrease in enzyme activity upon irradiation with a dose of 10 kGy was found. The possible mechanisms of the effects observed are discussed.
Subject(s)
Microsomes, Liver/enzymology , Oxidoreductases/antagonists & inhibitors , Animals , Dose-Response Relationship, Radiation , In Vitro Techniques , Lipid Peroxidation/radiation effects , Malondialdehyde/metabolism , Microsomes, Liver/metabolism , Microsomes, Liver/radiation effects , Rabbits , Radiation ToleranceABSTRACT
Electrons with energy 5 MeV have been studied for their effect on the electrokinetic properties of erythrocytes. The electrophoretic mobility of the cells was shown to decrease under the effect of radiation. It is supposed that the effect observed is determined by the structural changes of the membrane proteins.
