ABSTRACT
Aggregation process of riboflavin molecules in binary mixtures: water - dioxane, water - DMSO, and ethanol - isobutanol, were investigated using spectroscopic methods and quantum-chemical calculation. It was shown that at a constant concentration of riboflavin and different ratios of binary mixtures, a deformation of the electronic absorption spectra with a hypochromic effect is observed. The observed changes are caused by the formation of a hydrogen bond and dipole-dipole interaction between riboflavin molecules, which is accompanied by a shift and resonance splitting of excited electronic levels.
Subject(s)
Riboflavin , Vibration , Electronics , Hydrogen Bonding , Spectrum AnalysisABSTRACT
The influence of concentration, solvent nature on the intermolecular interaction and its spectroscopic manifestations in solutions of styrylcyanine dye Sbt ((E)-2-(4-(dimethylamino)styryl)-3-methylbenzo[d]thiazol-3-ium iodide) and its homodimers, i.e. dyes with two interconnected chromophores, was studied. It was found out that, depending on the concentration, the structure of dye molecules, the nature of the solvent various forms of associated molecules are forms; each of these forms is manifested in the spectra of absorption and fluorescence in its own way. It is shown that the intensity of the main absorption band of the studied dyes in aqueous solutions and in a mixture of water+ethanol decreases in the process of light irradiation and a new band is observed in the region of shorter wavelengths; the intensity of the new band increases with increase of radiation exposure of solutions.
Subject(s)
Benzothiazoles/chemistry , Coloring Agents/chemistry , Styrenes/chemistry , Benzothiazoles/radiation effects , Dimerization , Drug Stability , Light , Luminescence , Models, Biological , Photochemistry , Polymers/chemistry , Solutions/chemistry , Solvents/chemistry , Solvents/pharmacology , Spectrometry, Fluorescence , Styrenes/radiation effectsABSTRACT
The spectral-luminescent characteristics of newly synthesized styrylcyanine dyes on the base of dyes Sbo ((E)-2-(4-(dimethylamino)styryl)-3-methylbenzo[d]oxazol-3-ium iodide) and Sil ((E)-2-(4-(dimethylamino)styryl)-1,3,3-trimethyl-3H-indolium perchlorate) in aqueous solutions without and in the presence of bovine serum albumin (BSA) were studied. It was established that the absorption spectra of dyes Tol-6, Dbo-10 and Dil-10 with increasing amount of BSA appear new bands with λ(max)=505 nm, λ(max)=512 nm and λ(max)=566 nm, respectively, whose intensity increases in proportion to the amount of albumin. The intensity of the glow of the main band of fluorescence in the presence of BSA sharply increases. The binding constant (K) and the number of binding sites (N) of studied dyes with BSA were determined. The dependence of binding constants with BSA on the dipole moment of dye molecules was determined, which indicates that besides electrostatic forces of attraction between molecules styrylcyanine dyes with BSA, hydrophobic interactions are essential.
