[Study of the process of diffusion of gentin tablets through the artificial lipid barrier].

Currently during medical treatment of the patients suffering with gastrointestinal system diseases, particular attention is being devoted to the medical remedies of plant origin, which allow its protracted use during these diseases without expressed negative influence on important functions and structures of organism. Study of the dependence of the diffusion rate of active substances presented here as a multi-component plant origin dosage form, from the dissolution profile of solid dosage form in gastrointestinal tract, is an actual biopharmaceutical problem, which gives an information about the completeness of release of the active substances from the dosage form and its bioavailability. Gentin tablets developed at the department of pharmaceutical technology of Tbilisi State Medical University, presents the phytopreparation recommended for the prevention and treatment of the diseases of gastro-intestinal system. In-vitro study of the diffusion kinetics of active compounds from gentin tablets were performed using membrane model "SARTORIUS" Sm 16750 (FRG), which allows study of the diffusion rate of medical substances in the conditions closely imitating gastro-intestinal tract. Obtained experimental data showed that gamma-pyronic compounds are better absorbed in intestines than in stomach, as the value of diffusion rate constant k(d) in intestinal juice (pH=6,8) is 9, 17 x 10(-3) cm/min, while k(d) in gastric juice (pH=1,2) is 1, 63 x 10(-3) cm/min, thus the diffusion process is much more intensive in intestinal media with pH=6,8.

[Using of chromatographical methods to study stability, pharmacokinetics and metabolism of propofol (review)].

Propofol (somnopol, diprivan) 2,6-diisoprophilphenol is a preparation of short-term, intravenous narcosis used for local and general anaesthesia through single injection or permanent infusion with the speed of 4-12 mg/kg in an hour. In this study we tried to summarize the experience of using of chromatographical methods (gas chromatography-GCH0, high performance liquid chromatography (HPLC), thin layer chromatography (TLCH) and combination of mass-spectrometry with GCH (GCH-MC) and HPLC (HPL-MC) to study stability, pharmacokinetics and metabolism of propofol. A labour-intensive and important work have been carried out to obtain information about propofol.

[Investigation of conjugation and metabolism of propofol with the method of high performance liquid chromatography].

Propophol (somnopol, diprivan) is widely used in medicine for local and general narcosis through single injection or permanent infusion. Study of conjugation and metabolism of medications is considered as question of great importance, especially for substances used at anaesthesia. To study the above-mentioned questions the total complex of methods of chromatographical analysis, especially, high performance liquid chromatolgraphy (HPLC), are widely used. Objects of investigation were: blood plasma and urine of surgical patients. Method of investigation - HPLC with Ultraviolet-Detector. For investigation of structures of propophol conjugates and its metabolites selective enzymatic and non-selective acid hydrolysis were used. Two metabolites: 2,6-diisoprophil-1,4-bensoxinon and 2,6-diisopropilhydroxinon were determined.

[New methods of chemical and toxicological study of clonidine (Clophelinum)].

Clophelinum (clonidine) has hypotensive and soothing effect. The literature describes cases of a poisoning with clonidine, the majority of which are of the criminal character. Symptoms of clonidine intoxication differ from poisoning with other substances. Clinical picture of poisoning with clonidine is complex enough. Pathological and histological changes in internal organs in case of poisoning with clonidine are non specific. The methods of sampling, isolation, and qualitative determination need improvement. The aim of the research was investigation of chemical and toxicological properties of Clophelinum to develop new techniques of isolation and identification of clonidine. The technique of isolation is characterized by high degree of isolation (96 %), and technique of identification by high sensitivity (0,1 mkg/ml), which proves their efficiecy.

[Ecological immunodeficiency: immunogenetic aspects of its etiology and correction]. / Ekologicheskii immunodefitsit: immunogeneticheskie aspekty ego razvitiia i korektsii.

The examinations of auto-transport Kazakh drivers have indicated that there is a significant reduction in some immunological parameters HLA-B5 and HLA-DR5-proliferative responses of lymphocytes to mitogens, production of interleukin-1 and interleukin-2, activity of NK and LAK-cells. It is suggested that these impairments occur with their long-term exposure to automobile transport effluents (ATE), since the same changes in immunological parameters were found previously in the experiments with animals exposed to ATE for a long time. Some of the detected immunoresponsive disorders are associated with the availability of definite HLA antigens, such as HLA-B5 and HLA-P5. The new immunomodulating agents thymohexin (TH) and phyto-extraction drugs C4 and C6 used in vitro substantially restored the lower functional activity of immunocompetent cells and production of cytokines (thymohexin was particularly effective). The most marked recovery was observed in the drivers with the phenotype HLA-B6+ and HLA-P5+, i.e. in persons with maximally ATE-reduced immunological parameters.