ABSTRACT
Determination of halal food is essential in ensuring the tranquillity of consumers, especially Muslims. Halal products mean they are free from prohibited ingredients according to Islamic law. One ingredient that is prohibited is food products containing pork and its derivatives. An accurate verification method with a fast result is necessary to meet this requirement for halal food. DNA quantification of pork is now believed to be able to make accurate and quick decisions, as DNA acts as a reservoir or biological characterization of all living things, including pigs, according to specific characteristics of molecular and connection settings. Various DNA-based methods developed include PCR, biosensor and CRISPR methods. This review discussed various DNA-based Keywords: biosensor, CRISPR, detection, DNA, pork, PCR methods, including PCR, biosensor and CRISPR, to detect pork content in food. Among these methods, CRISPR is considered the easiest, fastest and most accurate. Therefore, it is important to develop this method further in the future. In this article, we provide a short review on DNA-based methods for detection of pork content in food products.
ABSTRACT
Microalgae-derived oils have potential as a biofuel feedstock. To produce microalgal oils at a large scale, large amounts of nutrients and energy are needed to grow the algae. In this study, we evaluated three types of agricultural fertilizer (AF)-based culture media (AF1, AF2, and AF3) based on a previously published enriched seawater (ES) medium to produce biomass and oils from Thalassiosira sp. Under laboratory conditions, the highest cell productivity of Thalassiosira sp. was obtained with the AF3 medium. Thalassiosira sp. cultured in the AF3 medium produced 10.4 ± 0.9 mg L-1 day-1oils, which is significantly higher than the 5.8 ± 0.7 mg L-1 day-1produced in the ES medium. The higher production was due to the presence of nitrate and trace elements, both of which played roles in enhancing biomass and oil content, respectively. During cell growth, resting spores appeared inside the cells and were a marker to harvest the cells. Because of the abundant availability of sunlight in the tropics during the year, the oil production of Thalassiosira sp. in the AF3 medium was scaled up using outdoor photobioreactors under different weather conditions (rainy and dry seasons). Thalassiosira sp. produced more unsaturated fatty acids during the rainy season and produced more saturated fatty acids during the dry season. This study also demonstrated that it was possible to culture Thalassiosira sp. under outdoor conditions using a low-cost agricultural fertilizer-based culture medium (AF3 medium) to produce biodiesel feedstock with an annual production of 8.1 ± 0.4 t ha-1 during the dry season and of 23.9 ± 6.8 t ha-1 during the rainy season.
