Production, characterization and therapeutic efficacy of egg yolk antibodies specific to Nosema ceranae.

Nosema disease, caused by Nosema ceranae, one of the single-celled fungal microsporidian parasites, is one of the most important and common diseases of adult honey bees. Since fumagillin, which has been used for decades in the control of Nosema disease in honey bees (Apis mellifera), poses a toxic threat and its efficacy against N. ceranae is uncertain, there is an urgent need to develop alternative prophylactic and curative strategies for the treatment of this disease. The main aim of this study was to investigate the therapeutic potential of specific egg yolk immunoglobulins (IgY) on Nosema disease. For this purpose, the presence of N. ceranae was determined by microscopic and PCR methods in honey bees collected from Nosema suspicious colonies by conducting a field survey. Layered Ataks chickens, divided into four groups each containing 20 animals, were vaccinated with live and inactivated vaccines prepared from field isolates of N. ceranae. Eggs were collected weekly for 10 weeks following the last vaccination. IgY extraction was performed using the PEG precipitation method from egg yolks collected from each group, and the purity of the antibodies was determined by SDS-PAGE and Western Blot. The presence of N. ceranae-specific IgYs was investigated by Western Blot and indirect ELISA methods. It was determined that specific IgYs showed high therapeutic efficacy on Nosema disease in naturally infected bee colonies. In addition, honey bees collected from infected colonies were brought to the laboratory and placed in cages with 30 bees each, and the effectiveness of IgYs was investigated under controlled conditions. It was detected that specific IgY reduced the Nosema spore load and the number of infected bees significantly in both the field and experimental study groups treated for seven days. It was concluded that chicken IgYs, an innovative and eco-friendly method, had a significant potential for use as an alternative to antifungal drugs.

Agility Skills, Speed, Balance and CMJ Performance in Soccer: A Comparison of Players with and without a Hearing Impairment.

This study investigates the differences in agility, speed, jump and balance performance and shooting skills between elite hearing-impaired national team soccer players (HISP) and without-hearing-impairment elite soccer players (woHISP). Players were divided into two groups, the HISP group (n = 13; 23.5 ± 3.1 years) and the woHISP group (n = 16; 20.6 ± 1.4 years), and were tested in three sessions, seven apart, for metrics including anthropometrics, speed (10 m, 20 m and 30 m), countermovement jump (CMJ), agility (Illinois, 505, zigzag), T test (agility and shooting skills), and balance. The results showed that 30 m, 20 m and 10 m sprint scores, agility/ skills (sec), shooting skills (goals), zigzag, Illinois, and 505 agility skills, and countermovement jump scores were significantly lower among players with hearing impairments (p < 0.05). There were no significant T test differences between HISP and woHISP (p > 0.05). The HISP showed right posterolateral and posteromedial, and left posterolateral and posteromedial scores that were lower than the woHISP group (p < 0.05). Anterior scores were not significantly different between each leg (p > 0.05). In conclusion, the HISP group showed higher performance scores for speed (10 m, 20 m and 30 m), CMJ, agility (Illinois, 505, zigzag) and T test (sec and goals), but not balance. Hearing-impaired soccer players are determined by their skill, training, and strategy, not their hearing ability.

Seed morphology using SEM techniques for identification of useful grasses in Dera Ghazi Khan, Pakistan.

In recent study, 15 taxa of family Poaceae from Dera Ghazi Khan were investigated for morphological characterization of seeds. Scanning electron microscopy (SEM) was utilized to study morphological features of grass seeds. Moreover, economic importance of studied taxa has been discussed in present research. Grass seeds or caryopsis were collected in their mature state from different areas of Dera Ghazi Khan. Collected caryopses were subjected to SEM to investigate surface sculpture, epidermal cell shape, and cell wall patterns. Caryopsis shape observed was elliptic to oval. Also, the morphology of plant is being investigated. Cell wall pattern observed was straight and wavy. Surface sculpturing varies from smooth to reticulate. Epidermal cell shape was either wavy or irregular. Hilum position is terminal in all species. Epidermal cell structure and cell wall pattern was not observable in some species. Major variations among studied taxa were observed in terms of seed surface pattern. Five types of caryopsis surface pattern were observed namely, striate, smooth, reticulate, papillate, and granulate. Investigated taxa illustrated variations in terms of studied morphological features. Utilization of SEM has been proved very helpful in exploring seed morphological features. These observed features can aid in delimitation and identification of various grass taxa. Recent research recommends the utilization of SEM for caryopsis morphology to solve the identification issue of problematic grass genera.

Reliability and Validity of a New Test of Agility and Skill for Female Amateur Soccer Players.

The aim of this study was to evaluate the Agility and Skill Test, which had been recently developed to assess agility and skill in female athletes. Following a 10 min warm-up, two trials to test the reliability and validity of the test were conducted one week apart. Measurements were collected to compare soccer players' physical performance in a 20 m sprint, a T-Drill test, the Illinois Agility Run Test, change-of-direction and acceleration, as well as agility and skill. All tests were completed following the same order. Thirty-four amateur female soccer players were recruited (age = 20.8 ± 1.9 years; body height = 166 ± 6.9 cm; body mass = 55.5 ± 5.8 kg). To determine the reliability and usefulness of these tests, paired sample t-tests, intra-class correlation coefficients, typical error, coefficient of variation, and differences between the typical error and smallest worthwhile change statistics were computed. Test results showed no significant differences between the two sessions (p > 0.01). There were higher intra-class correlations between the test and retest values (r = 0.94-0.99) for all tests. Typical error values were below the smallest worthwhile change, indicating 'good' usefulness for these tests. A near perfect Pearson correlation between the Agility and Skill Test (r = 0.98) was found, and there were moderate-to-large levels of correlation between the Agility and Skill Test and other measures (r = 0.37 to r = 0.56). The results of this study suggest that the Agility and Skill Test is a reliable and valid test for female soccer players and has significant value for assessing the integrative agility and skill capability of soccer players.

Assessment of hydration status of elite young male soccer players with different methods and new approach method of substitute urine strip.

BACKGROUND: The purpose of the study is to determine and compare the hydration status with different methods and determine fluid intake, dehydration percentages and sweat rate of 26 young male soccer players (15 ± 1.2 years) before an important competition. More specifically, the study aims at validating the urine strip and advising the players to use it as an easy and practical method. METHODS: Measurements of urine analysis were taken from the urine sample of the participants before breakfast and conducted for 3 consecutive days before the competition. Hydration status was assessed through analysis of urine color, urine specific gravity (USG) (laboratory, strip, refractometry), and osmolality. The players' dehydration percentages and sweat ratio were calculated. RESULTS: The average values for all samples were 3 ± 1 for color, and 1.021 ± 4 g/cm(3) for USG (laboratory), and 1.021 ± 3 g/cm(3) for USG (strip), and 1.021 ± 4 for USG (refractometry), and 903 ± 133 mOsm/kg for osmolality. USG (strip) was highly correlated with USG (laboratory), USG (refractometry) (r = 0.8; P < 0.01) and osmolality (r = 0.7; P < 0.01), and moderately correlated with urine color (r = 0.4; P < 0.05). The mean dehydration percentage and sweat rate of the soccer players were observed as 0.5 % and 582.3 ± 232.0 mL/h, respectively. CONCLUSION: We found that youth soccer players are under a slight risk of dehydration under moderate weather conditions. As indicated by the research results, determination of hydration status of athletes must be taken into account more carefully under moderate and hot weather conditions. In addition, hydration methods were compatible with one another as measured in this study.

Comparison of Physical and Physiological Profiles in Elite and Amateur Young Wrestlers.

The aim of this study is to examine the physical and physiological determinants of wrestling success between elite and amateur male wrestlers. The wrestlers (N = 126) were first assigned to 3 groups based on their competitive level (top elite, elite, and amateur) and then to 6 groups according to their body mass (light, middle, and heavy weight) and their competitive level (elite and amateur). Top elite and elite wrestlers had significantly (p ≤ 0.05) more training experiences and maximal oxygen uptake compared with the amateur group. In separating weight classes, light- and middle-weight elite (MWE) wrestlers had significantly (p ≤ 0.05) more training experience (7-20%) compared with the light- and middle-weight amateur (MWA) wrestlers. No significant differences were detected between elite and amateur groups (light-, middle-, and heavy-weight wrestlers) for age, body mass, height, body mass index, and body fat (p > 0.05), with the exception of height for heavy wrestlers. Leg average and peak power values (in watts and watts per kilogram) in MWE were higher than MWA (6.5 and 13%, p ≤ 0.05). Relative leg average power value in heavy-weight elite (HWE) (in watts per kilogram) was higher than heavy-weight amateur (HWA) (9.6%, p ≤ 0.05). It was seen that elite wrestlers in MWE and HWE statistically possessed a higher V̇O2max (12.5 and 11.4%, respectively) than amateur middle- and heavy-weight wrestlers (p ≤ 0.05). The results of this study suggest that training experience, aerobic endurance, and anaerobic power and capacity will give a clear advantage for the wrestlers to take part in the elite group.

Physical Fitness Differences between Freestyle and Greco-Roman Junior Wrestlers.

The aim of the present study was to examine physical fitness differences between Freestyle and Greco-Roman junior wrestlers. One hundred twenty-six junior wrestlers, comprising 70 Freestyle and 56 Greco-Roman wrestlers, participated in this study. The somatic and physical fitness profile included body mass, body height, body mass index, body composition, flexibility, maximal anaerobic power of the legs and arms, aerobic endurance, hand grip strength, leg and back strength, and speed. No significant differences were found in the anthropometric and physical features between Freestyle and Greco-Roman wrestlers. The Greco-Roman wrestlers had a significantly higher level of relative leg power, peak arm power, relative peak arm power, and relative average arm power than Freestyle wrestlers (p < 0.05). Greco-Roman wrestlers were significantly faster, had better agility, and had a greater level of leg strength than Freestyle wrestlers, but Freestyle wrestlers were more flexible than Greco-Roman wrestlers (p < 0.05). Discriminant function analysis indicated that peak arm power, agility, speed, and flexibility were selective factors for the differences between Freestyle and Greco-Roman wrestlers. In conclusion, the present study indicates that the differences between these wrestling styles promote physical fitness differences in elite wrestlers. The results reflect specific features of each wrestling style.

The prevalence of metabolic syndrome in different ethnic groups in Turkey.

OBJECTIVE: To determine the prevalence of metabolic syndrome and its risk factors in various ethnic groups in Istanbul, Turkey. METHODS: Study participants were aged ≥ 20 years. Risk factor components for metabolic syndrome were measured and its presence was determined in study participants. RESULTS: The study included 254 Greeks, 273 West Thracians, 275 East Turkistanis and 304 Armenians. The prevalence of metabolic syndrome was significantly different between groups (Greeks, 19.3%; West Thracians, 24.9%; East Turkistanis, 15.3%; Armenians, 20.4%), and increased with age in all groups. Low levels of high-density lipoprotein cholesterol (HDL-C) were found mainly in Greeks (females, 64.5%; males, 61.6%) and West Thracians (females, 75.8%; males, 73.1%). Among East Turkistanis, HDL-C and triglyceride levels were significantly higher compared with the other ethnic groups. Hypertension was the most frequently encountered component of metabolic syndrome in East Turkistanis. CONCLUSIONS: The prevalence of metabolic syndrome varied between ethnic groups living in the same geographical location. In Turkey, metabolic syndrome is common. It is important to determine differences between ethnic groups, as this will assist in identifying those at higher risk of developing coronary heart disease.

Comparison of a new test for agility and skill in soccer with other agility tests.

The purpose of this study was both to develop a novel test to measure run, shuttle run and directional change agility, and soccer shots on goal with decision making and to compare it with other agility tests. Multiple comparisons and assessments were conducted, including test-retest, Illinois, Zig-Zag, 30 m, Bosco, T-drill agility, and Wingate peak power tests. A total of 113 Turkish amateur and professional soccer players and tertiary-level students participated in the study. Test-retest and inter-tester reliability testing measures were conducted with athletes. The correlation coefficient of the new test was .88, with no significant difference (p> 0.01> 0.01) between the test results obtained in the first and second test sessions. The results of an analysis of variance revealed a significant (p < 0.01) difference between the T-drill agility and power test results for soccer players. The new agility and skill test is an acceptable and reliable test when considering test-retest reliability and inter-rater reliability. The findings in this study suggest that the novel soccer-specific agility and shooting test can be utilized in the testing and identification of soccer players' talents.

Assessment of hydration status by urinary analysis of elite junior taekwon-do athletes in preparing for competition.

The aim of this study was to assess over time the hydration status of taekwon-do athletes during a preparatory camp. Measures of urine osmolality, conductivity, specific gravity and colour were made on the first urine sample passed by the participants (n = 32) in the morning before breakfast. The urinary measurements were made on samples collected at the beginning of the camp, 5 days later and one day before competition. Body mass was also measured at the same instants. Body mass (mean +/- s) was essentially the same on each of the measurement days (62.6 +/- 12.2, 62.7 +/- 12.3 and 62.2 +/- 12.6 kg, respectively). Mean urine osmolality at the beginning of the camp was relatively high (998 +/- 171 mOsmol . kg-1), suggesting that a significant number of the athletes were already hypohydrated. However, no significant differences were detected in urine osmolality at the three time points during the study. There were no significant differences in any of the four methods of urine analysis during the study (P > 0.05). The average values for all samples were 989 +/- 205 mOsmol . kg-1 for osmolality, 25.5 +/- 6.7 mS . cm-1 for conductivity, 1.017 +/- 0.010 g . cm-3 for specific gravity and 4 +/- 1 arbitrary units for colour. Correlation analysis between the different methods suggested moderately good agreement (correlation coefficient = 0.5-0.7) between all four measurement techniques (P < 0.01). The coefficients of variation for these techniques were relatively low (CV = 11.8 - 35.0%). The results of this study suggest that some of the taekwon-do athletes were slightly hypohydrated in the morning on each of the test days, but there was no evidence to suggest that most of the athletes further restricted their fluid intake to make weight. In addition, it appears that each of the four methods used gave essentially the same estimate of hydration status of these athletes.

Moderate exercise combined with dietary vitamins C and E counteracts oxidative stress in the kidney and lens of streptozotocin-induced diabetic-rat.

Oxidative stress has a key role in the pathogenesis of diabetes-induced cataract formation and nephropathy. Daily moderate exercise and vitamins C and E (VCE) supplementation can be beneficial to diabetes due to reducing blood glucose and free radical production. The aim of this study was to analyze the effect of moderate exercise with vitamin VCE on lipid peroxidation (LP) and antioxidative systems in the kidneys and lens of streptozotocin-induced diabetic rats. Forty female Wistar rats were used. They were randomly divided into four groups. The first and second groups were used as control and diabetic groups. The third group was the diabetic-exercise group. VCE-supplemented feed was given to diabetic-exercise rats constituting the fourth group. Animals in the exercised groups were moderately exercised daily on a treadmill for three weeks (five days a week). Diabetes was induced on day zero of exercise. Body weights in the four groups were recorded weekly. Lens and kidney samples were taken from all animals on day 20. Glutathione peroxidase (GSH-Px), reduced glutathione (GSH), vitamin E, and beta-carotene levels in kidney and lens, albumin in plasma, and body weight were significantly lower in the diabetic group than in the control group, whereas there was a significant increase in LP of kidney and lens as well as plasma glucose, urea, and creatinine levels in the diabetic group. The decrease in antioxidant enzymes, vitamins, and albumin and the increase in LP and glucose levels in diabetic rats were significantly improved with exercise and VCE supplementation. In the diabetic animals, the decreased beta-carotene and vitamins A levels in kidney did not improve through exercise only, although their levels were increased by exercise plus VCE supplementation. In conclusion, these data demonstrate that lipid peroxidation increases in the lens and kidney of diabetic animals and this could be due to decreases in antioxidant vitamins and enzymes. However, dietary VCE with moderate exercise may strengthen the antioxidant defense system through the reduction of ROS and blood glucose levels. The VCE supplementations with exercise may play a role in preventing the development of diabetic nephropathy and cataract formation in diabetic animals.

Moderate exercise with a dietary vitamin C and E combination protects against streptozotocin-induced oxidative damage to the blood and improves fetal outcomes in pregnant rats.

A considerable amount of clinical and experimental evidence now exists suggesting that many biochemical pathways strictly associated with diabetes increase the production of reactive oxygen species (ROS). However, daily moderate exercise and vitamins C and E (VCE) supplementation can be beneficial to diabetes due to reducing blood glucose and free radical production. In the present study, we investigated the effect of VCE and moderate exercise on lipid peroxidation (MDA) and scavenging enzyme activity in the blood of STZ-induced diabetic pregnant rats. Fifty female Wistar rats were used and were randomly divided into five groups. The first and second were used as the control and pregnant control groups, respectively. The third group was the pregnant diabetic group. The fourth group was the diabetic-pregnant-exercise group. VCE-supplemented feed was given to pregnant-diabetic-exercise rats constituting the fifth group. Animals in the exercised groups were moderately exercised daily on a treadmill (16.1 m/min, 45 min/d) for three weeks (five days a week). Diabetes was induced on day zero of the study. Body weights in the five groups were recorded weekly. Plasma and red blood cell (RBC) samples were taken from all animals on day 20. Glutathione peroxidase (GSH-Px), catalase and reduced glutathione (GSH) levels in plasma and RBCs, glucose, vitamins A, E and beta-carotene in plasma, the number of fetuses, and body weight were lower in pregnant diabetic rats than in control animals, whereas there was a significant increase in platelet counts and plasma and RBC MDA levels. The decreased antioxidant enzymes did not improve through exercise only. However, the decreased vitamins, and increased MDA, glucose levels and white blood cell (WBC) counts were improved either by exercise or exercise plus VCE supplementation. There were no significant changes in the RBC counts and hemoglobin values in the five groups. In conclusion, these data demonstrate that there is an increase in MDA in the blood of diabetic pregnant animals, whereas there is a decrease in the number of fetuses, antioxidant vitamins and enzymes. However, dietary VCE with moderate exercise may strengthen the antioxidant defense system due to reducing ROS. They may play a role in preventing diabetes-related diseases of pregnant subjects.