ABSTRACT
NMR spectroscopy was applied for studying the products of glucose and sorbitol oxidation by cells of Gluconobacter oxydans. An analysis of 1H NMR spectra showed that the transformation of glucose results in the formation of diketogluconic acid, and sorbitol is oxidized to sorbose. In the 32P NMR spectra, only a signal of inorganic phosphate was detected, which accumulated in the medium as a result of cell lysis.
Subject(s)
Gluconobacter oxydans/growth & development , Glucose/metabolism , Sorbitol/metabolism , Biotransformation , Gluconobacter oxydans/chemistry , Glucose/analysis , Magnetic Resonance Spectroscopy/methods , Sorbitol/analysis , Sorbose/analysis , Sorbose/metabolismABSTRACT
By the high resolution NMR method was made comparative research of carboanhydrase B, alpha-lactalbumin and apomyoglobin in a molten globular state. It is shown that though in an observed spectra the typical signals at 5.7-5 ppm, responding to the signals of alpha-CH protons of the beta-structure, method of spin diffusion proves that after all is taking place in carboanhydrase B and alpha-lactalbumin, that corresponds with the data of CD.